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Method for obtaining decidua basalis mesenchymal stem cells and kit

A technology of stem cells and decidua, applied in the field of bioengineering, can solve problems to be improved, etc.

Inactive Publication Date: 2017-03-22
SOUTH CHINA INSTITUDE OF BIOMEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, current methods for obtaining decidua basal mesenchymal stem cells still need to be improved

Method used

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  • Method for obtaining decidua basalis mesenchymal stem cells and kit
  • Method for obtaining decidua basalis mesenchymal stem cells and kit
  • Method for obtaining decidua basalis mesenchymal stem cells and kit

Examples

Experimental program
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Effect test

Embodiment

[0032] In this embodiment, decidua basal mesenchymal stem cells were obtained according to the following method:

[0033] (1) Use a scalpel to cut off a layer of decidua basalis along the placental leaflet, put it in a petri dish filled with ice saline, wash off the blood as much as possible, put it in a vial and cut it to a size of 2cm× 2cm, then transfer 5g of cut decidua basalis to a 50ml centrifuge tube;

[0034] (2) Add 5ml of mixed enzyme preparation to each tube, which contains 0.25% trypsin, 2μg / mL collagenase IV and 0.1 mass% collagenase II, shake at 37°C and 150rpm for 30min, then add FBS to stop Digestion with trypsin, and adding physiological saline to the obtained digested product, diluting 5 times to obtain a dilution;

[0035] (3) Filter the diluted solution through a 100-mesh sieve, collect the filtrate, centrifuge at 2000 rpm for 5 minutes, discard the supernatant, add 2ml of normal saline to resuspend, and then dilute to a total volume of 5ml to obtain a cel...

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Abstract

The invention discloses a method for obtaining decidua basalis mesenchymal stem cells and a kit. The method for obtaining the decidua basalis mesenchymal stem cells comprises the following steps: carrying out digestion treatment on decidua basalis by utilizing a mixed enzyme preparation, so as to obtain digested products; and separating the decidua basalis mesenchymal stem cells from the digested products, wherein the mixed enzyme preparation comprises pancreatic enzymes, collagenase IV and collagenase II. By adopting the method for obtaining the decidua basalis mesenchymal stem cells, large quantity of decidua basalis mesenchymal stem cells with higher purity and higher activity can be obtained.

Description

technical field [0001] The invention relates to the field of bioengineering. Specifically, the present invention relates to a method and a kit for obtaining decidua basal mesenchymal stem cells. Background technique [0002] Decidua basalis is the decidua that is in contact with the blastocyst trophoblast, and constitutes the maternal part of the placenta together with trophoblast cells. As an important source of maternal mesenchymal stem cells, decidua basalis mesenchymal stem cells (DBMSC) can be safely used For the treatment of immune system diseases in the mother. At the same time, because of its multi-directional differentiation ability, decidual mesenchymal stem cells can also be used for autologous or allogenic tissue regeneration. [0003] However, the current methods for obtaining decidua basal mesenchymal stem cells still need to be improved. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems existing ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00
Inventor 裴雪涛岳文贾雅丽苏如玉庞晓敏曹宁
Owner SOUTH CHINA INSTITUDE OF BIOMEDICINE
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