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Application of leonurine in preparation of medicine for improving infertility pregnancy outcome

A technology of leonurine and infertility, applied in the field of application of leonurine in the preparation of medicines for improving the pregnancy outcome of infertility

Pending Publication Date: 2020-07-07
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leonurine is the specific active ingredient of traditional Chinese medicine Leonurine. It has been reported that Leonurine can relieve the pain sensitivity of adenomyosis model mice. Reports of Repeated Implantation Failures in IVF-ET

Method used

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  • Application of leonurine in preparation of medicine for improving infertility pregnancy outcome
  • Application of leonurine in preparation of medicine for improving infertility pregnancy outcome
  • Application of leonurine in preparation of medicine for improving infertility pregnancy outcome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] Example 1 Experiment of Leonurine Inhibiting Mouse Endometriosis Focus

[0121] Fifty-four 6-week-old C57 / B6 female mice (donors) were intraperitoneally injected with 0.2 mg / kg / day of estradiol (E2). Three days after the administration, the mice were sacrificed, and the uteri were isolated. The uterine tissue was shredded mechanically, and the uterine fragments were intraperitoneally injected into another 54 8-week-old C57 / B6 female mice (recipients); one week later, the 54 recipient mice were randomly divided into 3 groups, and low doses of motherwort were injected intraperitoneally (7.5mg / kg / d) or high dose of motherwort (15mg / kg / d), mice in the control group were intraperitoneally injected with a corresponding volume of placebo saline. One week after the administration, the mice were killed, and the endometriosis foci in the abdominal cavity were separated, measured and weighed; the results were as follows: figure 1 As shown, the size and weight of abdominal endome...

Embodiment 2

[0122] Example 2 Experiment of Leonurine Inhibiting Fibrosis of Mouse Endometriosis Focus and Reducing Cystic Wall Thickness

[0123] like figure 2 As shown in -A, the mouse endometriosis lesions are stained by Masson, which can present blue collagen; after a week of intraperitoneal injection, in the endometriosis model with daily intraperitoneal injection of 7.5mg / kg motherwort In mice, the degree of fibrosis of the endometriosis lesion interstitium was significantly reduced compared with the placebo-administered endometriosis model mice (one-way analysis of variance: P figure 2 As shown in -B, the mouse endometriosis lesion was stained by hematoxylin-eosin, and the cystic wall structure of the ectopic focus can be shown clearly; In the endometriosis model mice, the wall thickness of endometriosis lesions was significantly reduced compared with the endometriosis model mice given to the control group (one-way analysis of variance: P<0.05); the experimental results proved that...

Embodiment 3

[0124] Example 3 Leonurine induces apoptosis of stromal cells in ectopic lesions of mouse endometriosis model and inhibits proliferation experiment

[0125] The paraffin sections of mouse endometriosis lesions were stained by immunohistochemistry for Ki67 and P53 respectively, and the positive cells were brown; as image 3 As shown in -A, after a week of intraperitoneal injection, in the endometriosis model mice with daily intraperitoneal injection of 7.5mg / kg and 15mg / kg leonurine, the expression of the proliferation-related molecule Ki67 in the stromal cells of the ectopic focus Expression was significantly lower than that of endometriosis model mice given placebo (one-way analysis of variance: P image 3 As shown in -B, after a week of intraperitoneal injection, in the endometriosis model mice with daily intraperitoneal injection of 7.5mg / kg and 15mg / kg of motherwort, the apoptosis-related molecule P53 was expressed in the stromal cells of ectopic lesions The expression in e...

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Abstract

The invention belongs to the field of modern pharmacy of traditional Chinese medicines, and relates to application of a specific monomer leonurine in preparation of a medicine for improving infertility pregnancy outcome. The leonurine is injected into the abdominal cavity during an EMS (acute renal syndrome) modeling period, so that the volume and weight of an ectopic focus can be remarkably reduced, local fibrosis, invasion and transfer are inhibited, and the pregnancy rate of an EMS model mouse can be remarkably increased. The leonurine inhibits proliferation and invasion of endometrium stromal cells by inducing apoptosis of the endometrium stromal cells, adjusts expression balance of a local estrogen-progestin receptor of the ectopic focus, activates EMS model pelvic and abdominal immune cells, enhances phagocytic and killing functions of the cells, reverses poor ecdysis of the EMS model mouse, has similar effects on growth and invasion of ectopic focus ESC of a human EMS patient and expression of the estrogen-progestin receptor, does not affect growth and activity of the endometrium ESC of a normal child-bearing female, hormone receptor expression, ecdysis process and proliferation, apoptosis and cell activity of human early-pregnancy embryo trophoblast, and can be used for preparing an effective prevention and treatment medicine for EMS combined infertility patients.

Description

technical field [0001] The invention belongs to the field of modern Chinese medicine pharmacy, and relates to the application of a Chinese herbal medicine motherwort extract, leonurine, in the preparation of medicines, in particular to the use of motherurine in the preparation of medicines for improving the outcome of infertility and pregnancy, and in particular to the specific monoclonal compound in the Chinese herbal medicine motherwort. The application of Leonurine (Leo) in the preparation of medicines for preventing and treating endometriosis and improving the pregnancy outcome of infertility patients caused by endometriosis, said Leonurine is especially suitable for preparing endometriosis Preparing medication for patients with hypochondriac during pregnancy or before IVF-ET (test-tube baby) transplantation. Background technique [0002] The prior art discloses that endometriosis (Endometriosis, EMS) refers to a common female gynecological disease formed by the implanta...

Claims

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Application Information

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IPC IPC(8): A61K31/235A61P15/00A61P15/08
CPCA61K31/235A61P15/00A61P15/08
Inventor 杜美蓉朱依谆林懿空李芸芸刘新华
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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