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63 results about "Immune recognition" patented technology

Immune Recognition is a modified compilation of an experimental leukocyte culture conference about various aspects of macrophage and lymphocyte biology in relation to the eponymous central theme. The book is divided into nine sections. Section I covers non-antigenic signals and receptors for lymphocyte activation;

Determining Antigen Recognition through Barcoding of MHC Multimers

The present invention describes the use of nucleic acid barcodes as specific labels for MHC multimers to determine the antigen responsiveness in biological samples. After cellular selection the barcode sequence will be revealed by sequencing. This technology allows for detection of multiple (potentially >1000) different antigen-specific cells in a single sample. The technology can be used for T-cell epitope mapping, immune-recognition discovery, diagnostics tests and measuring immune reactivity after vaccination or immune-related therapies.
Owner:IMMUDEX APS +1

Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof

InactiveCN102507929AReduced Quantitative DetectionHigh sensitivityMaterial analysisImmune recognitionImmunity
The invention discloses a signal-enhancement type immunochromatographic gold-labeled test strip and a preparation method thereof. The preparation method of the gold-labeled test strip comprises the following steps of: (1) combining colloidal gold nanometer particles with different particle diameters; (2) preparing a colloidal gold immunity detection probe; (3) preparing a colloidal gold signal enhancement probe; (4) mounting signal-enhancement type test paper; and (5) establishing a detection method of the signal-enhancement type test paper. The signal-enhancement type immunochromatographic gold-labeled test strip, disclosed by the invention, can be applied to field fast ultra-sensitive detection without needing other auxiliary instruments, and has the following advantages that: a detection operation can be finished within 10-15 minutes and a detection result can be obtained; the sensitivity of the traditional test paper detection method can be remarkably improved, and the ultra-sensitive detection of a low-concentration appointed target object which is not realized by the traditional test paper is realized; and as long as an antibody of the immunity identification probe of the test paper is correspondingly replaced, the detection of other target objects can be realized; the specificity is high, the stability is good, the application range is wide, the cost is low and the like; in addition, the signal-enhancement type immunochromatographic gold-labeled test strip and the preparation method thereof, disclosed by the invention, are easy to promote and apply.
Owner:HEFEI UNIV OF TECH

Preparation and application of adjacent hybridization dual-mode immunosensor based on MXene nanosheet photo-thermal amplification

ActiveCN110716040AIncrease analytical capacityGood biocompatibilityBiological material analysisBiological testingDeoxyribonucleic acid probeImmune recognition
The invention discloses preparation and application of an adjacent hybridization dual-mode immunosensor based on MXene nanosheet photo-thermal amplification. Polyethyleneimine (PEI) modified TiO2 disc-shaped nanoparticles are used as a substrate, and a large amount of capture substrate deoxyribonucleic acids are fixed on a sensing interface through electrodeposition of gold. Two deoxyribonucleic acid probes are both labeled with a human epididymis protein 4 (HE4) antibody of a target object, and after the deoxyribonucleic acid probes as a recognition part of HE4 are captured by DNA3, the HE4 induces adjacent hybridization between primary antibody-labeled DNA1 and secondary antibody-labeled DNA2 by immunorecognition. An MXene nanosheet is loaded with a large amount of thionine, so that theMXene nanosheet can be effectively intercalated into a double-stranded DNA groove formed by hybridization, can be used as a signal probe for electrochemical-temperature dual modes, and realizes amplification of electrochemical signals due to temperature rise generated by the photo-thermal effect. Under the irradiation of an 808 nm infrared laser device, high-sensitivity detection of the HE4 is realized.
Owner:FUJIAN NORMAL UNIV

Difunctional composite nanosphere and method for rapidly detecting food-borne pathogenic bacteria

The invention provides a difunctional composite nanosphere and a method for rapidly detecting food-borne pathogenic bacteria. The difunctional composite nanosphere is characterized in that silicon dioxide is adopted and quantum dots and magnetic nanoparticles are embedded simultaneously to construct the composite nanosphere having the optical property and the superparamagetism. The corresponding quantum dot and the nanosphere are connected with a monoclonal antibody capable of specifically recognizing food-borne pathogenic bacteria, and an immune quantum dot probe capable of carrying out antigen-antibody reaction with antigens on the surface of the bacteria and an immune composite nanosphere probe are acquired. The composite nanosphere with the composite structure can be used as a carrier for immunologically recognizing and separating the pathogenic bacteria and also can be used as a signal enhancer element of the immune quantum dot probe, secondary amplification of a detection signal is realized, and target microorganisms of a sample to be detected are acquired by adopting an optical detection method. According to the method, the detection time less than or equal to 2h can be greatly shortened, the sensitivity (102cfu/mL) is improved, and the method is suitable for on-site rapid detection of foods and environment samples and can be popularized and applied in grass roots.
Owner:SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI

Preparation method of photoelectrochemical immunosensor for detecting procalcitonin

The invention relates to a preparation method of a photoelectrochemical immunosensor for detecting procalcitonin. Through a split-type photoelectrochemical sensor construction mode, immune recognitionanalysis and photoelectric response test analysis of an inorganic semiconductor material are separated to achieve a purpose that the photoelectric test can be realized without damaging the immune recognition process of biomolecules; a bismuth stannate nano-material modified a cadmium sulfide nano-material is used as a substrate material to provide basic photoelectric response, and band gap structures of the two are matched, so that visible light utilization efficiency can be well improved; in the antigen and antibody specific immune recognition process in a 96 microwell plate, acetylcholin esterase is firmly bound to a procalcitonin secondary antibody through an aminated aldehyde silicon dioxide material, and the compound marks the procalcitonin secondary antibody as a marker; after acetylcholine iodide is dropped into the 96-microwell plate, the acetylcholine esterase has catalytic reaction with the acetylcholine iodide to obtain a product thiocholine, which serves as an electron donor to capture holes in the material caused by light excitation, so that light current is improved to different degrees, and sensitive detection of the procalcitonin is realized; and the detection limit of the procalcitonin is 0.20 pg / mL.
Owner:UNIV OF JINAN

Immune memory learning control based wet coagulation bath temperature control process for carbon fiber precursor

The invention provides an immune memory learning control based wet coagulation bath temperature control process for a carbon fiber precursor. The process route is as follows: polyacrylonitrile original liquid is extruded into a coagulation bath tank through a spinneret plate, the temperature of coagulating liquid in the coagulation bath tank is subjected to real-time detection by a temperature detecting element and is fed back to a controller, the controller carries out current control quantity calculation according to the current and historical data of set value input, control quantity and feedback output and outputs a current control quantity to a controlled object, a coagulation bath is heated, and finally, the actual temperature of the coagulation bath reaches a set temperature. The controller is an intelligent temperature controller based on immune memory learning and is used for simulating an immune recognition, response and memory mechanism of a human body immune system, the traditional iterative learning control algorithms are improved, interference is taken as an antigen and is subjected to recognition, elimination and feature memory, and a control system can quickly respond and accurately control interference when the same interference occurs again, so that the stability and interference immunity of the control system are further improved.
Owner:DONGHUA UNIV

Construction of live attenuated Shigella vaccine strains that express CFA/I antigens (CfaB and CfaE) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic E. coli

With the goal of creating a combination vaccine against Shigella and other diarrheal pathogens we have constructed a prototype vaccine strain of Shigella flexneri 2a (SC608) that can serve as a vector for the expression and delivery of heterologous antigens to the mucosal immune system. SC608 is an asd derivative of SC602, a well-characterized vaccine strain, which has recently undergone several phase 1 and 2 trials for safety and immunogenicity. Using non-antibiotic asd-based plasmids, we have created novel constructs for the expression of antigens from enterotoxigenic E. coli (ETEC), including CFA / I (CfaB and CfaE) and the B-subunit from heat-labile enterotoxin (LTB) in Shigella vaccine strain SC608. Heterologous protein expression levels and cellular localization are critical to immune recognition and have been verified by immunoblot analysis. Following intranasal immunization (SC608(CFAI) and SC608(CFAI / LTB) of guinea pigs, serum IgG and IgA immune responses to both the Shigella LPS and ETEC antigens can be detected by ELISA. In addition, ELISPOT analysis for ASCs from cervical lymph nodes and spleen showed similar responses. All vaccine strains conferred high levels of protection against challenge with wild-type S. flexneri 2a using the Sereny test. Furthermore, serum from guinea pigs immunized with SC608 expressing CfaB and LTB contained antibodies capable of neutralizing the cytological affects of heat-labile toxin (HLT) on Chinese Hamster Ovary (CHO) cells. These initial experiments demonstrate the validity of a multivalent invasive Shigella strain that can serve as a vector for the delivery of pathogen-derived antigens.
Owner:UNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY

Intelligent power distribution network fault recovery method based on organism immune mechanism

The invention relates to an intelligent power distribution network fault recovery method based on an organism immune mechanism, and the method specifically comprises the following steps: S1, an immune recognition module obtains information of a power distribution network through monitoring, judges the fault state, and extracts a characteristic quantity; S2, an immune matching module matches the characteristic quantity with a characteristic quantity in a fault state recorded in a power distribution network fault characteristic library in an immune memory module, if matching succeeds, the step S3 is executed, or otherwise, the step S4 is executed; S3, an immune response module performs secondary power grid fault response and executes a corresponding fault recovery scheme; S4, the immune response module performs primary power grid fault response, judges whether a fault downstream load has an interconnection switch for transfer, if so, performs load transfer and checks power flow, or otherwise, performs island division on a fault downstream area; and S5, a fault recovery scheme is generated according to the fault processing flow in the step S4, and the fault recovery scheme is recorded to a grid fault feature library. Compared with the prior art, the method has the advantages of improving the response speed of power grid fault recovery and the like.
Owner:SHANGHAI UNIVERSITY OF ELECTRIC POWER

Method for constructing spiral immunization assembly sensor for determining neomycin

The invention discloses a method for constructing a spiral immunization assembly sensor for determining neomycin, belonging to the technical field of material chemistry. The method comprises the following steps: preparing a water-soluble spiral polymer, modifying the spiral polymer with semi-antigen, coupling gold nano particles with antibody, and constructing a neomycin sensor. The invention provides a water-soluble spiral polymer, that is, poly(4-isocyano-benzoic acid). According to the invention, by immunization identification (neomycin semiantigen modified spiral polymer) and gold nanoparticle (coupled with antibody) assembly, the circular dichroism spectrum (CD) signals generated by the assembly detect the neomycin.
Owner:MEIZHENG BIO TECH CO LTD

Zwitterionic polypeptide, derivatives thereof and nano-drug based on zwitterionic polypeptide and derivatives thereof

ActiveCN111560049AExcellent and stable ability to entrap hydrophobic drugsFacilitated releaseOrganic active ingredientsPeptide preparation methodsTumor targetTumor targeting
The invention discloses a zwitterionic polypeptide, a derivative of the zwitterionic polypeptide and a nano-drug based on the zwitterionic polypeptide. Nano-drugs can be prepared on the basis of the zwitterionic polypeptide or derivatives thereof, the secondary structure of the zwitterionic polypeptide has excellent conversion capability before and after drug release, and release of the drugs in cells can be accelerated. The obtained nano-drug can be used for tumor targeted therapy; unexpected tumor targeting performance is obtained; and the nano-drug has excellent blood compatibility, has thecapability of avoiding immune recognition, tumor targeting, intracellular transformation and entering into cell nuclei, can reduce the distribution in liver, kidney, spleen, lung, heart and other organs rich in reticuloendothelial tissues, and finally realize the capability of efficiently inhibiting tumor growth and the in-vivo low-toxicity targeting tumor treatment effect.
Owner:ZHEJIANG UNIV

Glycoprotein dynamic light scattering immunization method based on phenylboronic acid cross-linking agent

The invention relates to a glycoprotein dynamic light scattering immunization method based on a phenylboronic acid cross-linking agent. The phenylboronic acid cross-linking agent and the magnetic carrier marked by the immune recognition element are used as a dynamic light scattering signal enhancement probe to form a multilayer sandwich structure by taking target glycoprotein as a bridge, and the average hydration kinetic particle size change of the solution before and after the multilayer sandwich structure is used as a dynamic light scattering signal to be output; and the content of glycoprotein in the to-be-detected sample is determined by utilizing the diameter change of hydration kinetics. According to the glycoprotein dynamic light scattering immunization method based on the phenylboronic acid cross-linking agent, the operation steps are simple, high-sensitivity detection of glycoprotein in a complex sample can be achieved in a short time, the prepared immunomagnetic beads can separate and enrich target protein from a complex sample matrix, and interference of the sample matrix on follow-up detection is effectively eliminated. The problem that the paired antibody is difficult to prepare can be solved, the glycoprotein can be simply, conveniently, rapidly and timely detected, and the method is worthy of being further popularized and used.
Owner:NANCHANG UNIV

An Isomorphism Recognition Method of Mechanism Kinematic Chain Based on Immune Genetic Hybrid Algorithm

InactiveCN103324983BOvercome the disadvantage of easy convergence to local optimumDiversity guaranteedGenetic modelsSpecial data processing applicationsAlgorithmImmune recognition
The inventiondiscloses a mechanism kinematic link isomorphism identification method based on the immunity genetic hybrid algorithm. The mechanism kinematic link isomorphism identification method includes the steps of (1) forming a topological graph corresponding to a mechanism kinematic link according to the structural mode of the mechanism kinematic link, (2) setting a genetic code of an adjacent matrix according to the topological graph, (3) taking adjacent matrixes, to be determined, of the two topological graphs, determining an objective function, (4) generating the initial antibody population at random, calculating adaptability of each individual, sequencing the individuals according to the adaptability, (5) taking the optimal saving strategy on the initial antibody population, (6) calculating the concentration of each individual, enabling the each individual concentration to be equal to a value obtained by dividing the total individual number in a population by the adjacent individual sum, (7) taking the optimal interaction strategy on the antibody population obtained in the step (5), (8) conducting the mutation operation, adopting the inverse operator to achieve the mutation operation, (9) combining the local search operator and the genetic algorithm, (10) forming the immunity genetic hybrid algorithm, and conducting the isomorphism identification operation on the mechanism kinematic link. The mechanism kinematic link isomorphism identification method overcomes the shortcoming that the immunity genetic hybrid algorithm can be easily restrained to local optimum.
Owner:JIANGSU UNIV

Method to augment immune system in response to disease or injury

In one example, the present invention comprises a deliberate insult or the recognition that an insult has occurred in a patient, which insult induces an immune recognition event. The T cell repertoire is catalogued from samples of the patient's lymphocytes from before and after the insult in order to detect and sequence the TCR alpha and beta loci of highly expanded T cell clonotypes. In some examples, this information is used in turn to generate autologous T cells or to create autologous genetically-engineered T cells, either of which include TCR sequences that target the individual's disease or injury, resulting in cure or amelioration of symptoms.
Owner:BARKEN ISRAEL +1

Grass carp bacteria small peptide recognition receptor as well as preparation method and application thereof

The invention discloses a grass carp bacteria small peptide recognition receptor and a preparation method and application thereof, the grass carp bacteria small peptide recognition receptor is grass carp NOD2-LRR protein, the amino acid sequence is as shown in SEQ ID NO.1, and the cDNA sequence of the coding gene is as shown in SEQ ID NO.2. The preparation method of the grass carp bacterium small peptide recognition receptor comprises the following steps: cloning a grass carp NOD2 gene, expressing, purifying and renaturating. In the invention, an immune recognition model of grass carp NOD2 on bacterial MDP is established by using a molecular docking method for the first time, NOD2-LRR protein is successfully obtained by using a prokaryotic expression system, and the result shows that the NOD2-LRR protein has binding activity on bacterial MDP, can specifically recognize bacterial MDP, and plays an important role in immune recognition of bacterial MDP; the method has potential application value in the aspects of developing novel antibacterial agents, immunopotentiators, feed additives and the like.
Owner:CHANGSHA UNIVERSITY
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