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Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof

A technology of gold-labeled test paper and immunochromatography, which is applied in the field of signal-enhanced ultra-sensitive colloidal gold-labeled immunochromatography test strips and its preparation, and can solve the problems that large-scale instruments cannot be popularized and used, the detection cost is high, and large-scale instruments are expensive. problem, to achieve high specificity, low cost and good stability

Inactive Publication Date: 2012-06-20
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of large-scale instruments, the operators must have relevant professional knowledge, and the high cost of detection determines that the detection methods relying on large-scale instruments cannot be widely used in various grassroots and on-site detections.

Method used

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  • Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof
  • Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof
  • Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A method for preparing a signal-enhanced bisphenol A immunochromatographic gold standard test strip. The specific steps are as follows:

[0034] (1) Preparation of rabbit anti-bisphenol A gold-labeled immunoprobe

[0035] A colloidal gold solution with a particle size of 40nm was selected, and potassium carbonate (K 2 CO 3 ) solution to adjust the pH value of the colloidal gold solution to 8.5 to obtain the adjusted colloidal gold solution A;

[0036] Mix the rabbit anti-bisphenol A antibody solution with a concentration of 1 mg / mL and the bovine serum albumin (BSA) solution with a concentration of 1 mg / mL at a molar ratio of 1:100 to obtain a mixed solution;

[0037] Take 10 μL of the mixed solution and add it to 1 mL of the adjusted colloidal gold solution A, and shake it at room temperature for 15 minutes;

[0038] Centrifuge at 10,000 rpm for 20 min at 4°C to remove unbound antibodies and bovine serum albumin (BSA) in the supernatant;

[0039] The precipitate ob...

Embodiment 2

[0051] A method for preparing a signal-enhanced phthalate immunochromatographic gold standard test strip. The specific steps are as follows:

[0052] (1) Preparation of rabbit anti-phthalate gold-labeled immunoprobe

[0053] Select a colloidal gold solution with a particle size of 30nm, adjust the pH value of the colloidal gold solution to 9.5 with a potassium carbonate (K2CO3) solution with a concentration of 0.1 M, and obtain the adjusted colloidal gold solution A;

[0054] Mix the rabbit anti-phthalate antibody solution with a concentration of 2 mg / mL and the bovine serum albumin (BSA) solution with a concentration of 2 mg / mL at a molar ratio of 1:100 to obtain a mixed solution;

[0055] Take 10 μL of the mixed solution and add it to 1 mL of the adjusted colloidal gold solution A, and shake it at room temperature for 15 minutes;

[0056] Centrifuge at 10,000 rpm for 20 min at 4°C to remove unbound antibodies and bovine serum albumin (BSA) in the supernatant;

[0057] The ...

Embodiment 3

[0069] A preparation method of a signal-enhanced rabbit anti-lycodopamine immunochromatographic gold standard test strip The specific operation steps are as follows:

[0070] (1) Preparation of rabbit anti-lycodopamine gold-labeled immune probe

[0071] A colloidal gold solution with a particle size of 30nm was selected, and potassium carbonate (K 2 CO 3 ) solution to adjust the pH value of the colloidal gold solution to 9.5 to obtain the adjusted colloidal gold solution A;

[0072] Mix the rabbit anti-lycodopamine antibody solution with a concentration of 2 mg / mL and the bovine serum albumin (BSA) solution with a concentration of 2 mg / mL at a molar ratio of 1:100 to obtain a mixed solution;

[0073] Take 10 μL of the mixed solution and add it to 1 mL of the adjusted colloidal gold solution A, and shake it at room temperature for 15 minutes;

[0074] Centrifuge at 10,000 rpm for 20 min at 4°C to remove unbound antibodies and bovine serum albumin (BSA) in the supernatant;

...

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Abstract

The invention discloses a signal-enhancement type immunochromatographic gold-labeled test strip and a preparation method thereof. The preparation method of the gold-labeled test strip comprises the following steps of: (1) combining colloidal gold nanometer particles with different particle diameters; (2) preparing a colloidal gold immunity detection probe; (3) preparing a colloidal gold signal enhancement probe; (4) mounting signal-enhancement type test paper; and (5) establishing a detection method of the signal-enhancement type test paper. The signal-enhancement type immunochromatographic gold-labeled test strip, disclosed by the invention, can be applied to field fast ultra-sensitive detection without needing other auxiliary instruments, and has the following advantages that: a detection operation can be finished within 10-15 minutes and a detection result can be obtained; the sensitivity of the traditional test paper detection method can be remarkably improved, and the ultra-sensitive detection of a low-concentration appointed target object which is not realized by the traditional test paper is realized; and as long as an antibody of the immunity identification probe of the test paper is correspondingly replaced, the detection of other target objects can be realized; the specificity is high, the stability is good, the application range is wide, the cost is low and the like; in addition, the signal-enhancement type immunochromatographic gold-labeled test strip and the preparation method thereof, disclosed by the invention, are easy to promote and apply.

Description

technical field [0001] The invention belongs to the technical field of gold-labeled test strips for food safety, inspection and quarantine, environmental detection and early disease diagnosis, and specifically relates to a signal-enhanced ultra-sensitive colloidal gold-labeled immunochromatographic test strip and a preparation method thereof. Background technique [0002] With the development of society and the increase in the volume of international import and export trade, inspection and quarantine and food safety testing are facing more and more testing tasks, and the large amount of testing samples puts forward new requirements for inspection and quarantine and food safety testing. The traditional detection method relying on instruments has the advantages of high detection sensitivity and good stability of detection results. However, due to the high price of large-scale instruments, the operators must have relevant professional knowledge, and the high cost of detection d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531
Inventor 陈伟胥传来郑磊刘永胜余晓峰陈寒清梅占龙吴晶晶
Owner HEFEI UNIV OF TECH
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