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Preparation method of photoelectrochemical immunosensor for detecting procalcitonin

An immunosensor, procalcitonin technology, applied in the direction of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of long detection time and limitations, achieve the effect of short response time, low detection limit, and promote rapid transfer

Active Publication Date: 2019-11-26
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, radioimmunoassay detection and chemiluminescence detection are time-consuming, and the use of radioimmunoassays is limited by the contamination of radioactive elements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of photoelectrochemical sensor

[0046] Dissolve 0.8 g tin tetrachloride pentahydrate in 10 mL ultrapure water, adjust the pH of the above solution to 4 with 3 M aqueous sodium hydroxide solution, stir for 10 minutes, and centrifuge to obtain a white precipitate; dissolve the obtained precipitate In 30 mL of ultrapure water containing 1.8 g bismuth nitrate pentahydrate, adjust the pH to 11 with 3 M aqueous sodium hydroxide solution, continue stirring for 10 minutes, then transfer the solution to an autoclave, and react at 160 °C for 12 hours , naturally cool to room temperature after the reaction is over, then the product is washed 3 times with absolute ethanol and ultrapure water, and the final product is dried overnight at 40 ° C to obtain a bismuth stannate material;

[0047] (2) Preparation of cadmium sulfide material

[0048] Dissolve 0.4 g of sodium sulfide in 20 mL of water, stir for 5 minutes, and use this solution as solution A; dissol...

Embodiment 2

[0067] Embodiment 2 Framework of photoelectrochemical sensor

[0068] (1) Preparation of bismuth stannate material

[0069] Dissolve 0.9 g of tin tetrachloride pentahydrate in 20 mL of ultrapure water, adjust the pH of the solution to 4 with 4 M aqueous sodium hydroxide solution, stir for ten minutes, and centrifuge to obtain a white precipitate; dissolve the obtained precipitate in Contain 1.94 g of bismuth nitrate pentahydrate in 40 mL of ultrapure water, then adjust the pH of this solution to 12 with 4 M. sodium hydroxide aqueous solution, continue to stir for 15 minutes, then transfer this solution to a high-pressure reactor, at 180 ° C Reacted for 24 hours, naturally cooled to room temperature after the reaction was completed, then the product was washed 3 times with absolute ethanol and ultrapure water, and the final product was dried overnight at 60 ° C to obtain a bismuth stannate material;

[0070] (2) Preparation of cadmium sulfide material

[0071] Dissolve 0.48 g...

Embodiment 3

[0090] Example 3 Construction of Photoelectrochemical Immunosensor

[0091] (1) Preparation of bismuth stannate material

[0092] Dissolve 1.0 g of tin tetrachloride pentahydrate in 30 mL of ultrapure water, adjust the pH of the solution to 6 with 5 M sodium hydroxide solution, stir for 20 minutes and then centrifuge to obtain a white precipitate; dissolve the obtained precipitate in Contain 2.0 g bismuth nitrate pentahydrate in 50 mL ultrapure water, then adjust the pH of this solution to 13 with 5 M sodium hydroxide aqueous solution, continue stirring for 20 minutes, then transfer this solution to an autoclave, and react at 200 °C After 24 hours, the reaction was naturally cooled to room temperature, and the product was washed 3 times with absolute ethanol and ultrapure water, and the final product was dried overnight at 60 ° C to obtain a bismuth stannate material;

[0093] (2) Preparation of cadmium sulfide material

[0094] Dissolve 0.50 g of sodium sulfide in 30 mL of ...

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Abstract

The invention relates to a preparation method of a photoelectrochemical immunosensor for detecting procalcitonin. Through a split-type photoelectrochemical sensor construction mode, immune recognitionanalysis and photoelectric response test analysis of an inorganic semiconductor material are separated to achieve a purpose that the photoelectric test can be realized without damaging the immune recognition process of biomolecules; a bismuth stannate nano-material modified a cadmium sulfide nano-material is used as a substrate material to provide basic photoelectric response, and band gap structures of the two are matched, so that visible light utilization efficiency can be well improved; in the antigen and antibody specific immune recognition process in a 96 microwell plate, acetylcholin esterase is firmly bound to a procalcitonin secondary antibody through an aminated aldehyde silicon dioxide material, and the compound marks the procalcitonin secondary antibody as a marker; after acetylcholine iodide is dropped into the 96-microwell plate, the acetylcholine esterase has catalytic reaction with the acetylcholine iodide to obtain a product thiocholine, which serves as an electron donor to capture holes in the material caused by light excitation, so that light current is improved to different degrees, and sensitive detection of the procalcitonin is realized; and the detection limit of the procalcitonin is 0.20 pg / mL.

Description

technical field [0001] The invention relates to a preparation method of a photoelectrochemical immunosensor for detecting procalcitonin, specifically, bismuth stannate sensitized by cadmium sulfide is used as a base material, and acetylcholinesterase linked to silicon dioxide is used as a marker to mark procalcitonin. The invention relates to a procalcitonin secondary antibody, which is a combined photoelectrochemical sensor for detecting procalcitonin, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Procalcitonin (PCT, proclacitonin), a sepsis-inducible protein, is a hormonally inactive calcitonin propeptide substance that can be used as a marker of infectious inflammation to distinguish bacterial infection from viral Sexual infection, and to judge the severity and prognosis of bacterial infection. Compared with the indicators routinely used in the diagnosis of inflammation, PCT has been recognized as...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3275
Inventor 魏琴冷东全范大伟吴丹曹伟马洪敏张勇
Owner UNIV OF JINAN
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