Method for separating, culturing and purifying endometrial cells
A technology of endometrium and purification method, which is applied in the direction of cell dissociation method, cell culture active agent, biochemical equipment and method, etc., which can solve the problems of small number of adherent cells, long replacement cycle, decreased cell activity, etc., and achieve The effect of improving the efficiency of isolation and culture
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[0030] A method for separating, culturing and purifying endometrial cells, comprising the following steps:
[0031] 1. Take the exfoliated endometrial tissue, add cleaning solution to clean it, add red blood cell lysate to the cleaning solution to remove blood, cut it into pieces with straight scissors, and set aside;
[0032] Wherein, the cleaning solution is prepared by physiological saline containing 50% erythrocyte lysate.
[0033] 2. Take the shredded endometrial tissue in step 1, add digestion solution I, digest at 37°C for 1.5 hours, stop digestion with endometrial cell culture medium after digestion, then filter through a 400-mesh filter, separate and retain the filtrate and filter residue, and centrifuge the filtrate: the centrifugal force is 300g, the time is 5min, and the precipitate obtained after centrifugation is resuspended with endometrial cell culture medium;
[0034] Wherein, the digestive solution I was prepared by adding 3 mg / mL type I collagenase and 0.3 ...
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