The invention discloses four
cell types which have wide heteroplastic
transplantation application prospect and can be effectively induced into induced pluripotent stem (iPS) cells. The origins of three
cell types are
placental tissue, namely
amnion mesenchymal
cell, chorion mesenchymal cell and
umbilical cord mesenchymal cell; and the origin of the other one is
amniotic fluid cell. Besides, the invention also discloses an induction
reprogramming method for producing cells capable of producing induced pluripotent stem (iPS) cells by efficient induction, including the following steps: cDNA containing pluripotent
stem cell factor is respectively introduced into the four
primary culture cells; the four primary cells in which cDNA is introduced are respectively cultured on appropriate
culture mediums, primary iPS is obtained and then quality of iPS is optimized on appropriate
culture mediums, and
cloning of pluripotent
stem cell can be primarily evaluated. In the invention, three mesenchymal cells of
placenta origin and
amniotic fluid cell all can be induced into iPS, wherein the chorion mesenchymal cell is compared with
fibroblast, efficiency of induction
reprogramming is improved by over 100 times, efficiency is about 2.3%, and the efficiency is slightly higher than that of horny cell; and a means which is more effective and more pertinent is provided for building
disease model, screening
drug and controlling oriented differentiation.