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Anal fistula patch or plug, as well as preparation method and application thereof

A technique for anal fistula and allogeneic body is applied in the field of tissue engineering of biomedical materials to achieve the effects of prolonging degradation and digestion, improving flexibility, good elasticity and toughness

Active Publication Date: 2017-05-31
BEIJING JAYYALIFE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation method of this patent does not propose corresponding treatment for the above technical problems

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  • Anal fistula patch or plug, as well as preparation method and application thereof
  • Anal fistula patch or plug, as well as preparation method and application thereof
  • Anal fistula patch or plug, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation method of acellular allodermal matrix

[0062] The present invention provides a method for preparing an acellular allogeneic dermal matrix. Specifically, a preferred preparation embodiment includes the following steps in order:

[0063] Put the raw material into 0.2% trypsin-dispase enzyme solution (w / v) for soaking and shaking, wherein the mass ratio of trypsin to dispase enzyme is 2:1, adjust the pH value to 7.4 with PBS buffer solution, and the shaking speed is 100rpm, Soak for 4 hours to get the semi-finished product A.

[0064] The semi-finished product A is put into a container containing surfactant and hypertonic saline solution, soaked in ultrasonic to obtain the semi-finished product B. Ultrasonic immersion conditions are: 40KHz, 220W, ultrasonic for 6 minutes, soak for 3 hours, repeat 3 times; the formula containing surfactant and hypertonic saline solution contains 0.2g / L polyethylene glycol and SDS. Among them, polyethylene glycol The...

Embodiment 2

[0072] Example 2 Preparation method of acellular allogeneic dermal matrix

[0073] The present invention provides a method for preparing an acellular allogeneic dermal matrix. Specifically, a preferred preparation embodiment includes the following steps in order:

[0074] Put the raw material into 0.3% trypsin-papain solution (w / v), soak and shake, wherein, the mass ratio of trypsin and papain is 2.5:1, adjust the pH value to 7.2 with PBS buffer solution, and the shaking speed is 100rpm , Soak for 4 hours to get the semi-finished product A.

[0075] The semi-finished product A is put into a container containing surfactant and hypertonic saline solution, soaked in ultrasonic to obtain the semi-finished product B. The ultrasonic soaking conditions are: 50KHz, 220W, ultrasonic for 5 minutes, soak for 2 hours, repeat 4 times; the formula containing surfactant and hypertonic saline solution contains 0.2g / L polyethylene glycol and Triton X-100 among them The selected molecular wei...

Embodiment 3

[0083] Example 3 Preparation method of acellular allogeneic dermal matrix

[0084] The present invention provides a method for preparing an acellular allogeneic dermal matrix. Specifically, a preferred preparation embodiment includes the following steps in order:

[0085] Put the raw material into 0.2% trypsin and bromelain solution (w / v) for soaking and shaking, wherein the mass ratio of trypsin and bromelain is 1:1, adjust the pH value to 7.4 with PBS buffer solution, and the shaking speed is 100rpm, Soak for 4 hours to get the semi-finished product A.

[0086] The semi-finished product A is put into a container containing surfactant and hypertonic saline solution, soaked in ultrasonic to obtain the semi-finished product B. The conditions of ultrasonic immersion are: 60KHz, 220W, ultrasonic for 8 minutes, soak for 3 hours, repeat twice; the formula containing surfactant and hypertonic saline solution contains 0.2g / L polyethylene glycol and SDS. Among them, polyethylene glyc...

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Abstract

The invention provides an anal fistula patch or plug, as well as a preparation method and application thereof. The anal fistula patch or the anal fistula plug is prepared from an acellular dermal matrix. The acellular dermal matrix is obtained by performing protein enzyme solution digestive treatment, surfactant-containing hypertonic saline acellular treatment, crosslinking treatment, DNA degradation treatment and amino acid nutritional treatment on allogenic skin. The invention further provides application of the anal fistula patch or the anal fistula plug to preparation of a biological material for treating anal fistula. The acellular dermal matrix derives from human skin tissue, special physicochemical and biochemical treatment is conducted, all the components which may cause immunologic rejection are removed, the fiber three-dimensional stent structure of the original tissue is retained integrally, new vessels and fibroblasts grow quickly after implanting, and a good clinical effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to an anal fistula patch or suppository, a preparation method and an application thereof. Background technique [0002] Anal fistula is a chronic infective conduit connecting the perianal skin and the rectum / anal canal, and consists of three parts: the internal opening, the fistula, and the external opening. Most anal fistulas are primary anal fistulas, which arise from a nonspecific infection of the anal glands that develop after the formation of a perianal abscess. Anal fistula is clinically manifested as recurrent perianal infection, ulceration and pus, and perianal cancer may occur in patients who do not heal for a long time. Anal fistula is one of the common anorectal canal diseases. It can occur at any age, and it is more common in men aged 30-50, with a male:female ratio of 2:1. Sainio reports an annual incidence of 8.6 / 100,000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/362A61L27/3687A61L27/50
Inventor 孙继煌石清东吴文华
Owner BEIJING JAYYALIFE BIOTECH CO LTD
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