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Human liquid state derma preparation for injection and its preparation method

A technology for injection and dermis, applied in the field of medical bioengineering, can solve the problems of slow time, usually 4-6 months, low collagen content, inconvenient active ingredients, etc., to achieve remarkable cosmetic curative effect, quick effect, and effective effect lasting effect

Inactive Publication Date: 2008-11-19
赵紫电
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another disadvantage of this method is that Ringer's solution is inconvenient to maintain the activity of the active ingredient for a long time, and it needs to be used soon after being formulated into an injection, which is inconvenient in actual use
The disadvantage of this technology is that the collagen content in the injection that can immediately perform the filling and repairing functions of human soft tissues is low, resulting in slow results after injection into scars and wrinkles, which generally takes 4-6 months; Regulators may also have doubts about the safety of using animal serum as a component of culture fluid

Method used

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  • Human liquid state derma preparation for injection and its preparation method
  • Human liquid state derma preparation for injection and its preparation method
  • Human liquid state derma preparation for injection and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Preparation of Human Soft Tissue Filler----For Wrinkle Removal Treatment (20 cases)

[0050] 1. Extraction of autologous healthy skin samples

[0051] Disinfect the back of the ear with 70% alcohol, and then perform local topical anesthesia with 10% lidocaine and 1 / 100,000 epinephrine. Use a skin picker or a scalpel and surgical scissors to remove the 4 square millimeters of epidermis and dermis tissue behind the ear of the recipient, put them in a tissue preservation solution (DMEM cell preservation solution, Hyclone, USA), and make a needle at the wound site Stitched, Band-Aid covered.

[0052] 2. Preparation of cell suspension (tissue digestion culture method)

[0053] 1. Tissue digestion (Forma, or Sanyo CO 2 Incubator): place the skin tissue block in a 35mm petri dish, add 1ml of 0.05% trypsin / EDTA solution, 37°C, 10 minutes; DMEM+10%FBS solution to stop.

[0054] 2. Cell separation: Cut up the digested tissue pieces and break them up with a straw. ...

Embodiment 2

[0085] Example 2: Preparation of Human Soft Tissue Filler - Removal of Depressed Scars (20 cases)

[0086] 1. Extraction of autologous healthy skin samples

[0087] Disinfect the back of the ear with 70% alcohol, and then perform local topical anesthesia with 10% lidocaine and 1 / 100,000 epinephrine. Use a skin picker or a scalpel and surgical scissors to remove the 4 square millimeters of epidermis and dermis tissue behind the ear of the recipient, put them in a tissue preservation solution (DMEM cell preservation solution, Hyclone, USA), and make a needle at the wound site Stitched, Band-Aid covered.

[0088] 2. Preparation of cell suspension

[0089] 1. Tissue block culture method: chop the tissue block into 1×1mm 2 , tiled in a 35mm culture dish, add 2ml of the above-mentioned dermal cell culture fluid (Sciencell company, trade mark is 2301, wherein the main component is the basic culture fluid, the concentration of growth factor EGF is 1ng / ml, the concentration of nutri...

Embodiment 3

[0115] Example 3: Preparation of Human Soft Tissue Filler——Improvement and Cosmetology for Skin Matrix (20 cases)

[0116] 1. Extraction of autologous healthy skin samples

[0117] Disinfect the back of the ear with 70% alcohol, and then perform local topical anesthesia with 10% lidocaine and 1 / 100,000 epinephrine. Use a skin picker or a scalpel and surgical scissors to remove the 4 square millimeters of epidermis and dermis tissue behind the ear of the recipient, put them in a tissue preservation solution (DMEM cell preservation solution, Hyclone, USA), and make a needle at the wound site Stitched, Band-Aid covered.

[0118] 2. Preparation of cell suspension

[0119] 1. Tissue block culture method: chop the tissue block into 1×1mm 2 , tiled in a 35mm culture dish, add 2ml of the above-mentioned dermal cell culture solution (Sciencell company, the brand is 2301, wherein the main component is the basic culture solution, the concentration of the growth factor EGF is 1ng / ml, t...

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Abstract

The invention relates to a human body liquid state corium layer preparation for injection and a preparation method thereof, and belongs to the field of medical bio engineering. The preparation method of the human body liquid state corium layer preparation for injection comprises the following steps: digesting and separating the skin from a curer himself; obtaining corium fibroblast stem cell, a fibroblast fore body cell and a fibroblast cell; performing the extraneous omnirange blood serum-free cultivation and expansion of the cells; preparing effective component and normal saline injection or other injection collected as well as other suitable components selected arbitrarily. The effective component is composed of at least one of the corium f fibroblast stem cell, the fibroblast fore body cell and the fibroblast cell collected after being cultivated and collagen. In each-liter filling agent for injection, the total quantity of the effective cell component is 10,000,000 to 80,000,000, and the collagen contains 10-100 mg / ml filling agent for injection. The human body liquid state corium layer preparation has the advantages that the used material is derived from the curer himself, no rejection reaction and side effect occur, the effect is quick after remedy, the effect is obvious, the expression is natural, the effect action is durable and can be kept for a plurality of years.

Description

technical field [0001] The invention relates to a human body liquid dermis preparation for injection and a preparation method thereof, more specifically a cellular tissue filler, which is used for filling and repairing human soft tissues, including removing wrinkles and repairing depressed scars. Its tissue filler contains autologous fibroblast stem cells, fibroblast precursor cells, fibroblasts and collagen after in vitro culture and expansion. Its preparation process uses serum-free culture materials, and finally prepares an injection suspension of tissue filler, which is injected into wrinkles, depressed scars, traumatic skin dermis, and lips to achieve the purpose of filling and repairing. field of bioengineering. Background technique [0002] The methods usually applied to human soft tissue filling can be divided into dermal filling and subcutaneous filling. These fillings are not autologous tissue living materials in the past, but biosynthetic materials or heterosexua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38
Inventor 赵紫电徐亚青
Owner 赵紫电
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