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Preparation method of artificial endometrium

An endometrium and artificial technology, which is applied in the field of preparation of artificial endometrium, can solve the problems of embryonic and maternal component growth, development and differentiation process changes, and cannot fully reproduce the shape, structure and function, etc.

Inactive Publication Date: 2005-05-18
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
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Problems solved by technology

In many embryo implantation models, most of them can only achieve the adhesion of embryonic components and maternal components and the epitaxial growth of trophoblasts. Few embryos or trophoblasts can actually invade maternal components. The main reason is that in vitro culture conditions and in vivo The situation is not exactly the same, so that the growth, development and differentiation process of the cultured embryos and maternal components are changed, and the shape, structure and function of the body cannot be completely reproduced

Method used

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  • Preparation method of artificial endometrium
  • Preparation method of artificial endometrium

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Experimental program
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Effect test

Embodiment

[0022] 1. Separation and purification of endometrial cells:

[0023] BALB / c mice, clean-grade experimental animals, aged 8-12 weeks, weighing 18g-20g, female: male = 1:1 cage mating, checked the vaginal plug in the next morning, and found that the vaginal plug was the first day of pregnancy

[0024] Isolation of endometrial cells: application of enzymatic digestion. 10 pregnant d4 mice were killed by decervicalization, the bilateral uterine horns were clipped, cut longitudinally and put into a petri dish filled with PBS, added 2.5% Pacreatin and 0.5% Trypsin, after 1.5h at 4°C, placed at 20°C for 0.5 h. Rotate with a vortex rotator for 10 sec, let it stand for 5 minutes, discard the upper 1 / 3 suspension, absorb the lower 2 / 3 cell suspension into a centrifuge tube containing fetal bovine serum, rinse the uterus twice with 5ml PBS, and absorb the supernatant, 1000rpm Centrifuge for 5min, discard the supernatant, add 10ml PBS to rinse the cells twice, and centrifuge at 1000rpm×5...

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Abstract

The present invention belongs to the tissue engineering field and is especially a preparation process of artificial endometrium. New type biodegradable material is used in making stereo rack, endometrium cell of experiment animal is used as seed cell, and artificial endometrium is in vitro constituted via special culture process. The artificial endometrium is a 3D structure, has the similar shapeand function to the in vivo endometrium, can secrete extracellular matrix component and can emulate the configuration and function of in vivo endometrium. The present invention provides the research of embryo nidatin mechanism with ideal platform and provides treating way for endometrium damage caused by induced abortion, tuberculosis, etc.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a method for preparing artificial endometrium. Background technique [0002] The mechanism and process of embryo implantation and embryo growth and development in the mother's body are still unclear. It is regulated by hormones, cytokines, proteins, enzymes and other information substances. It is a complex and precise process, and it is extremely difficult to study directly in vivo . At present, there have been studies on the establishment of in vitro models. In many embryo implantation models, most of them can only achieve the adhesion of embryonic components and maternal components and the epitaxial growth of trophoblasts. Few embryos or trophoblasts can actually invade maternal components. The main reason is that in vitro culture conditions and in vivo The situation is not exactly the same, so that the growth, development and differentiation process of the cultu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/00C12N5/071
Inventor 周剑萍刘银坤张炜王丽
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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