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Application of cyanidin-3-O-glucoside in preparation of medicine for treating and/or preventing endometrial hyperplasia disease

A technology for endometrial hyperplasia and endometrial thickening, applied in sexually transmitted diseases, drug combinations, and resistance to vector-borne diseases, etc. Improve the effect of increased thickness

Active Publication Date: 2020-11-24
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the improvement and therapeutic effect of C3G on Cd-induced endometrial thickening, and its protective mechanism is still unclear.

Method used

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  • Application of cyanidin-3-O-glucoside in preparation of medicine for treating and/or preventing endometrial hyperplasia disease
  • Application of cyanidin-3-O-glucoside in preparation of medicine for treating and/or preventing endometrial hyperplasia disease
  • Application of cyanidin-3-O-glucoside in preparation of medicine for treating and/or preventing endometrial hyperplasia disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 The protective effect of C3G on endometrium

[0032] 1. Grouping of animals

[0033] After adaptive feeding with ordinary AIN93-G feed for 10 days, the mice were randomly divided into four groups according to the weight stratification method: Control group, Cd group, C3G intervention group (Cd+60mg / kg C3G group, Cd+120mg / kg C3G group) , 10 in each group. The Control group was fed with ordinary AIN93-G feed and intragastrically administered normal saline every day; the Cd group was given ordinary AIN93-G feed while intragastrically administered 5.0mg / kg.bw CdCl every day 2 solution; Cd+60mg / kg C3G group and Cd+120mg / kg C3G group were given ordinary AIN93-G feed, and 60mg / kg C3G and 120mg / kg C3G were given intragastrically every day, and 5.0mg / kg.bw CdCl 2 solution. The mice in each group were given free access to water and food, and were treated for 58 consecutive days. The body weight was weighed every three days to adjust the gavage dose, and the feed was ...

Embodiment 2

[0054] Example 2 Effects of Cd and C3G on Uterine mRNA Transcriptome

[0055] 1. Experimental method

[0056] In this study, the II lumina sequencing platform was used to perform RNA-seq sequencing on uterine tissue, and the transcriptome was detected at the single base level; in addition, the differential genes between the Control group and the Cd group, the Cd group and the Cd+120mg / kg C3G group were analyzed.

[0057] Volcano plots are a way to visualize differentially expressed genes. The screening threshold of differential genes was set as: |log2(FoldChange)|>1 and p value<0.01.

[0058] The disorder of estrogen effect is an important way for Cd exposure to cause female reproductive damage. At the same time, the disorder of estrogen and progesterone action genes will lead to the occurrence of uterine related diseases such as endometriosis, so the changes of estrogen and progesterone action genes should be paid attention to.

[0059] 2. Experimental results

[0060] The...

Embodiment 3

[0065] Example 3 C3G relieves endometrial proliferation

[0066] 1. Experimental method

[0067] In the previous study, uterine HE-stained sections and RNA-seq sequencing showed that Cd exposure led to uterine proliferation and the improvement after C3G intervention was closely related to the cell proliferation pathway, so the uterine proliferation was first studied.

[0068] Proliferating Cell Nuclear Antigen (PCNA) is one of the markers of cell proliferation, and the expression level of PCNA was analyzed by WB and IHC.

[0069] 2. Experimental results

[0070] The expression analysis results of PCNA were as follows: Figure 5 (A) shows the immunohistochemical staining of uterine PCNA, indicating that PCNA is localized in uterine cells, (B) and (C) are the results of electrophoresis and analysis of uterine PCNA, indicating that C3G is important for Cd exposure The induced uterine proliferation is an obvious improvement; (D) and (E) are the electrophoresis and analysis resu...

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Abstract

The invention discloses application of cyanidin-3-O-glucoside in preparation of a medicine for treating and / or preventing an endometrial hyperplasia disease. The cyanidin-3-O-glucoside is found to becapable of improving endometrial hyperplasia and endometrial hyperproliferation caused by Cd exposure, and the cyanidin-3-O-glucoside intervenes in the process of protecting growth and development ofendometrial cells by maintaining the estrogen effect level, so that the uterus can normally grow, embryo implantation is ensured, and meanwhile, the cyanidin-3-O-glucoside has a protection effect on the endometrium. Therefore, the cyanidin-3-O-glucoside has a good application prospect in preparation of the medicine for treating and / or preventing the endometrial hyperplasia disease, preparation ofthe medicine for treating and / or preventing Cd-induced abnormal proliferation of the endometrial cells and preparation of the medicine for protecting the endometrium.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of cyanidin-3-O-glucoside in the preparation of medicines for treating and / or preventing endometrial hyperplasia diseases. Background technique [0002] Epidemiological studies have shown that the female infertility rate is as high as 15%, and the exposure of environmental pollutants is one of the important reasons for female infertility. Cadmium (Cadmium, Cd) is a heavy metal environmental pollutant that accumulates in the soil and can enter the human body through food, water, air and other channels. Embryo receptivity poses a potential threat. Therefore, there is an urgent need to provide a method capable of improving and treating Cd-induced endometrial hyperplasia. [0003] Endometrial hyperplasia has a certain tendency to become cancerous, so it is listed as a precancerous lesion. Clinically, the drugs for the treatment of endometrial h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P15/00
CPCA61K31/7048A61P15/00Y02A50/30
Inventor 白卫滨杨达城蒋鑫炜田灵敏孙建霞李旭升陈嘉莉
Owner JINAN UNIVERSITY
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