Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for processing bovine somatic cell cloned embryos constructed on basis of somatic cell nuclear transplantation

A technology of somatic cell nuclear transfer and processing method, which is applied in the field of animal somatic cell cloning, can solve the problems of reprogramming failure, incompleteness, low cloning efficiency and reprogramming, and achieve the effect of improving development rate and quality, and efficient production

Inactive Publication Date: 2013-04-24
NORTHWEST A & F UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it is believed that the main reason for the low cloning efficiency is that the nucleus of the donor somatic cell has not been completely reprogrammed by the cytoplasm of the recipient ooplasm, that is, the reprogramming failure or incomplete
No changes in gene sequence are involved in this reprogramming process, mainly changes in epigenetic modifications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for processing bovine somatic cell cloned embryos constructed on basis of somatic cell nuclear transplantation
  • Method for processing bovine somatic cell cloned embryos constructed on basis of somatic cell nuclear transplantation
  • Method for processing bovine somatic cell cloned embryos constructed on basis of somatic cell nuclear transplantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] In the method for processing bovine somatic cell cloned embryos constructed based on somatic cell nuclear transfer provided by the present invention, after the reconstruction body is constructed, the reconstruction body is treated with Oxamflatin to inhibit dehistone acetylase, which can significantly improve bovine somatic cell Developmental rate and quality of cloned blastocysts for efficient in vitro production of bovine somatic cell cloned embryos. The present invention will be further described in detail in conjunction with the specific operation process and comparative analysis results below, which is an explanation of the present invention rather than a limitation.

[0028] First give the source or preparation of the following reagents and culture / treatment solutions:

[0029] Oxamflatin, DMSO, trypsin, EDTA, penicillin, streptomycin, inorganic salts, and paraffin oil are products of Sigma; DMEM / F12 liquid medium and special-grade fetal bovine serum (FBS) are pro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for processing bovine somatic cell cloned embryos constructed on the basis of somatic cell nuclear transplantation, which comprises the following steps of: injecting bovine somatic cells to be transplanted into denucleated oocyte to carry out electrofusion; and an hour later after the electrofusion is completed, selecting the successfully fused bovine somatic cellcloned embryos and processing the bovine somatic cell cloned embryos for 12 hours by 1muM of Oxamflatin. By the method, the developmental rate and the quality of the bovine somatic cell cloned embryos can be obviously improved and the bovine somatic cell cloned embryos can be efficiently produced in vitro.

Description

technical field [0001] The invention belongs to the technical field of animal somatic cell cloning, and relates to a method for processing bovine somatic cell cloned embryos constructed based on somatic cell nuclear transfer. Background technique [0002] Somatic cell cloning is a technology with great research and commercial value, which can be applied to therapeutic cloning, disease models, human organ transplantation, animal transgenic research, protection of endangered animal breeds, expansion of excellent livestock breeds, etc. But so far the efficiency of somatic cell cloning is still not high, which significantly restricts the wide application of this technology. [0003] At present, it is believed that the main reason for the low cloning efficiency is that the nucleus of the donor somatic cell is not completely reprogrammed by the cytoplasm of the recipient ooplasm, that is, the reprogramming fails or is incomplete. No changes in gene sequence are involved in this r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/877
Inventor 苏建民张涌王勇胜
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com