Method for improving cattle cloning efficiency by micro ribonucleic acid
A technology of micro ribonucleic acid and high efficiency, which is applied in the direction of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as bovine cloning that has not been seen, and achieve the purpose of improving cloning efficiency, improving development rate and quality, and avoiding The effect of secondary damage
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[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.
[0026] miR-125b is a microRNA enriched in early embryos, which can inhibit the histone methyltransferase Suv39h1, thereby effectively removing the 3-methylation (H3K9me3) of histone H3 lysine K9 (H3K9me3) epigenetic modification. Transfection of miR-125b can effectively reduce the level of histone H3K9me3 epigenetic modification in cloned embryos during the zygotic activation period, so as to promote the normal development of somatic cell cloned embryos. Accordingly, the present invention adds bta-miR-125b mimic to the electrofusion solution of bovine somatic cell nuclear transfer, and can effectively introduce bta-miR-125b mimic into bovine somatic cell cloned embryos, and realize the transfection of miR- 125b. Thereby reducing the level of histone H3K9me3 epigenetic modificati...
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