36 results about "Chromosome segregation" patented technology

Aryl- and heteroaryl-substituted urea compounds useful in the treatment of diseases and conditions related to DNA damage or lesions in DNA replication are disclosed. Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division also are disclosed.

Substituted urea compounds useful in the treatment of diseases and conditions related to DNA damage or lesions in DNA replication are disclosed. Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division, also are disclosed.

Aryl- and heteroaryl-substituted urea compounds useful in the treatment of diseases and conditions related to DNA damage or lesions in DNA replication are disclosed. Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division also are disclosed.

The invention provides methods of determining the aggressiveness, prognosis and response to therapy for particular cancers, which include comparing the expression levels of one or a plurality of differentially expressed genes from one or more 5 functional metagenes, including a carbohydrate / lipid metabolism metagene, a cell signalling metagene, a cellular development metagene, a cellular growth metagene, a chromosome segregation metagene, a DNA replication / recombination metagene, an immune system metagene, a metabolic disease metagene, a nucleic acid metabolism metagene, a post-translational modification metagene, a protein 10 synthesis / modification metagene and a multiple networks metagene. The method disclosed herein may be particularly suitable as a companion diagnostic for cancer therapies.

Substituted urea compounds useful in the treatment of diseases and C1-3alkyleneOR3 conditions related to DNA damage or lesions in DNA replication are disclosed formula (I), wherein X1 is null, —O—, —S—, —CH2—, or —N(R1)—; X2 is —O—, . -£>. -, or —N(R1)—,—. . Y xs 0 or S; or =y represents two hydrogen atoms attached to a common carbon atom, —W is selected from the group consisting of heteroaryl, aryl, heterocycloalkyl, cycloalkyl, and C1-6alkyl substituted with a heteroaryl. or aryl group; R6 is —C≡C—R7 or heteroaryl; R8, R9, and R10, independently, are selected from the group consisting of halo, optionally substituted C1-6alkyl, C2-6alkenyl, C2-6alkynyl, OCP3, CF3, NO2, CN, NC, N(R3)2, OR3, CO2R3, C(O)N (R3)2, C(O)R3, N(R1)COR3, N(R1)C(O)OR3, N(R8)C(O)OR3, N(R1)C(O)C1-3alkyleneC(O)R3, N(R1)C(O)C1 -3alkyleneC(O)OR3, N(R1)C(O)C1-3alkyleneOR3, N(R1)C(O)C1-3alkyleneNHC(O)OR3, N(R1)C(O)C1-3alkyleneSO2.NR3, C1-3alkyleneOR3, and SR3; Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division also are disclosed.

Substituted urea compounds useful in the treatment of diseases and conditions related to DNA damage or lesions in DNA replication are disclosed. Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division, also are disclosed.

The identification of MreB as essential for bacterial chromosome segregation provides a new target for antibiotic action. The MreB function is useful in the development of screening assays for new antibiotics, which may use, for example, genetic mutants in MreB, tests of MreB mediated chromosome segregation, and the like. In one embodiment of the invention, the antibiotic is an isothiourea compound, which may comprise a polyhalogenated benzyl group, e.g. at the 4 position, the 2,4 position, etc. A pharmaceutical composition comprising an MreB targeted antibiotic as an active agent is administered to a patient suffering from a microbial infection, particularly bacterial infections.

The invention discloses a method for separating chromosomes in plant cells by using fluorescence in-situ hybridization. The method for separating the chromosomes in the plant cells comprises the following steps of: the fluorescence in-situ hybridization is carried out on the plant cells, the single chromosome of which is picked; by comparing with the corresponding fluorescence in-situ hybridization map of the normal plant cells, the picked single chromosome is defined to be which chromosome, thus separating a purpose chromosome. The method has the advantages of being capable of separating the chromosomes with similar types and specific bands, widening the separating range of the chromosomes, avoiding the unnecessary chemical processing in the manufacture process of the chromosome specimens, preventing the DNA of the chromosomes from being damaged, guaranteeing the completeness of the separated DNA. A microclone library of the whole chromosomes of the wheat can be obtained by applying the invention.

The identification of MreB as essential for bacterial chromosome segregation provides a new target for antibiotic action. The MreB function is useful in the development of screening assays for new antibiotics, which may use, for example, genetic mutants in MreB, tests of MreB mediated chromosome segregation, and the like. In one embodiment of the invention, the antibiotic is an isothiourea compound, which may comprise a polyhalogenated benzyl group, e.g. at the 4 position, the 2,4 position, etc. A pharmaceutical composition comprising an MreB targeted antibiotic as an active agent is administered to a patient suffering from a microbial infection, particularly bacterial infections.

The invention provides a single chromosome segregation method, a single chromosome high-throughput sequencing library construction method and application. According to the technical scheme, optimal dilution parameters are obtained through computer simulation experiments, chromosomes in single cell are segregated by a dilution method to obtain single chromosomes, and high-throughput sequencing and bioinformatics analysis are conducted so as to judge out that mutant genes derive from the father or the mother in homologous chromosomes. Compared with that the homologous chromosomes serve as a mixed sample for sequencing, the single chromosome segregation method has the advantages that the single chromosomes are segregated by the dilution method, and the single chromosome segregation method can be used for haplotype sequencing and has important application value in scientific research and clinical application.

Aryl- and heteroaryl-substituted urea compounds useful in the treatment of diseases and conditions related to DNA damage or lesions in DNA replication are disclosed. Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division also are disclosed. Formula (I).

The present invention is to provide meiosis-specific novel kinetochore protein Sgo1 (shugoshin) derived from fission yeast Schizosaccharomyces pombe, and a homologue or paralogue thereof having a regulatory activity of chromosome segregation; and DNAs encoding them; as a factor ensuring the retention of unidirection and cohesion in sister centromere at meiosis I in cooperation with cohesin. To elucidate the proteins protecting Rec8 during anaphase, the present inventor screened in fission yeast genes for a gene that inhibits mitotic growth and prevents sister chromatid from the separation at anaphase, when co-expressed with Rec8. In this approach, meiosis-specific protein Sgo1 that protects (Shugo) centromeric Rec8 from the degradation at anaphase I was indentified. Further, a budding yeast Sgo1 homologue and a fission yeast mitotic paralogue Sgo2 were identified.

The invention relates to a non-toxic extract composition GPR.1 capable of efficiently extracting plant genome DNA and an extraction method based on the extract. The method specifically comprises the following steps: lysing plant cells with an SDS extraction buffer containing PEG8000, PVPP (Polyvinylpolypyrrolidone) and protease K, performing chromosome segregation and protein denaturation, and releasing nucleic acids; adding potassium acetate to remove most of the proteins and polysaccharides, and continuously precipitating the DNA twice by adopting a PEG8000 / high-salt solution and an ethanol / high-salt solution so as to remove the polysaccharides, polyphenol, RNA, NTP and other impurities, thereby finally obtaining lots of high-purity DNA. According to the method disclosed by the invention, lots of high-purity genome DNA can be extracted from leaves (young leaves or mature leaves) of multiple plants, and the method is non-toxic to the operator, small in environmental pollution, low incost and short in time.

The present invention is generally directed to Matrimony (Mtrm) nucleotide sequences, polypeptides expressed from the nucleotide sequences and methods employing the Mtrm nucleotide and polypeptide sequences. The Mtrm nucleotide sequences of the invention express a dosage dependent polypeptide that mediates achiasmate chromosome disjunction. In particular, reduction in the amount of the Mtrm polypeptide will cause nondisjunction in the achiasmate chromosomes.

The invention provides a molecular marker and an identification method for identifying the chromosome segregation status of A05 and C04 of kale and beetroot interspecific hybrids and their progeny materials, belonging to the field of plant genetics and breeding. The invention provides a molecular marker and an identification method for identifying the chromosome segregation of A05 and C04 of kale and beetroot interspecific hybrids and their progeny materials. The marker is a pair of co-dominant molecular markers, and the marker is used to identify kale and red The true hybrids of vegetable moss interspecific hybrids can also be used to identify the self-progeny, backcross progeny, chromosome appendage lines, and recombination segregated populations and plants of distant hybrids.

Aryl- and heteroaryl-substituted urea compounds useful in the treatment of diseases and conditions related to DNA damage or lesions in DNA replication are disclosed. Methods of making the compounds, and their use as therapeutic agents, for example, in treating cancer and other diseases characterized by defects in DNA replication, chromosome segregation, or cell division also are disclosed.

The invention discloses a method for separating chromosomes in plant cells by using fluorescence in-situ hybridization. The method for separating the chromosomes in the plant cells comprises the following steps of: the fluorescence in-situ hybridization is carried out on the plant cells, the single chromosome of which is picked; by comparing with the corresponding fluorescence in-situ hybridization map of the normal plant cells, the picked single chromosome is defined to be which chromosome, thus separating a purpose chromosome. The method has the advantages of being capable of separating thechromosomes with similar types and specific bands, widening the separating range of the chromosomes, avoiding the unnecessary chemical processing in the manufacture process of the chromosome specimens, preventing the DNA of the chromosomes from being damaged, guaranteeing the completeness of the separated DNA. A microclone library of the whole chromosomes of the wheat can be obtained by applying the invention.

The invention discloses a molecular marker and method for identifying interspecific hybrid between Chinese cabbage and Ethiopian mustard and for tracking the chromosome separation of progeny materialsA03 and C03, and belongs to the field of plant heredity and breeding. The marker is a pair of co-dominant molecular markers which can be used for identifying the true and false of the interspecific hybrid between the Chinese cabbage and the Ethiopian mustard, and can also be used for identifying and tracking self cross progeny, backcross progeny, and chromosome addition lines of distant hybrid, and the A03 and C03 chromosomal segregation of recombinant isolated population and plants.

The invention discloses a molecular marker and a method for identifying the segregation of A06 and C05 chromosomes of brassica vegetable interspecific hybrids and offspring materials, belonging to the field of plant genetics and breeding. The molecular marker can quickly and accurately identify the interspecific hybrids of Brassica A genome (such as Chinese cabbage, red cabbage, etc.) . The method can identify distant hybrids of Brassica A genome and C genome plants and their backcross offspring, which can expand the genetic resources of Brassica vegetables, transfer new traits, enrich vegetable types, and enrich people's daily dietary nutrition.

The invention discloses a molecular marker and method for identifying interspecific hybrids between Chinese cabbages and Brassica carinata and tracking segregation of A02 chromosomes and C02 chromosomes in offspring materials of the interspecific hybrids, and belongs to the field of plant genetic breeding. The marker comprises a pair of codominant molecular marker bodies, and can be used for identifying the authenticity of the interspecific hybrids between Chinese cabbages and Brassica carinata, and can also be used for identifying and tracking selfing offsprings, backcross offsprings, chromosome addition lines and recombined segregation populations of distant hybrids and segregation of A02 chromosomes and C02 chromosomes in plants.

The invention provides a method for improving the in-vitro utilization rate of sheep oocyte, which comprises the following steps of: washing poor quality BCB-oocyte obtained by chromosome segregation for 2 to 3 times by using mature culture solution which contains 50 to 100mu Mol / L PDE3 inhibitor milrinone and culturing for 6 to 9 hours, and washing the BCB-oocyte for 2 to 3 times by using matureculture solution which does not contain the milrinone and culturing for 24 hours; removing granular cells around the oocyte, transferring the oocyte to cleaning solution, picking the oocyte and removing a first polar body, and counting the removal rate of the polar body, namely maturation rate; and activating the oocyte from which the first polar body is removed for 4 to 5 minutes by using 5mu Mol / L ionomycin and washing the oocyte for 1 to 3 times by using the cleaning solution, activating the oocyte for 2 to 3 hours by using 2mMol / L 6-methyl aminopurine which is balanced in a CO2 box 2 to 3 hours ago, washing the oocyte for 3 or 4 times by using the culture solution which is balanced in the CO2 box 2 hours ago, culturing the oocyte in the culture solution for 48 hours, counting cleavage rate, and counting blastocyst rate after 168 hours. In addition, the BCB-oocyte obtained by the chromosome segregation is washed by the mature culture solution for 2 to 3 times and cultured for 24 hours to be directly utilized. The method has great practical value for the in-vitro utilization of the sheep oocyte.

The invention is directed to isolated genomic polynucleotide fragments that encode human SNARE YKT6, human glucokinase, human adipocyte enhancer binding protein (AEBP1) and DNA directed 50 kD regulatory subunit (POLD2), vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain SNARE YKT6, human glucokinase, AEBP1 protein and POLD2 and to diagnose, treat, prevent and / or ameliorate a pathological disorder.

The present invention discloses a molecular marker and a method for identifying interspecific hybrids of Chinese cabbages and brassica carinata and tracking A10 and C07 chromosome segregation conditions of progeny materials of the interspecific hybrids, and belongs to the field of plant genetic breeding. The marker is a pair of co-dominant molecular markers, used for identifying authenticity of the interspecific hybrids of the Chinese cabbages and brassica carinata, and also can be used for identifying and tracking the A10 and C07 chromosome segregation conditions of self-bred progeny, backcross progeny, chromosome additional lines of distant hybrids , recombinant segregation populations and plants.