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Molecular marker for identifying A10 and C07 chromosome segregation conditions of interspecific hybrids of Chinese cabbages and brassica carinata, and progeny materials of interspecific hybrids

A molecular marker and chromosome technology, applied in the field of genetics and breeding, can solve problems such as time-consuming and labor-intensive, and achieve the effect of simple and fast cost, low technical requirements, and expansion of genetic resources.

Active Publication Date: 2019-11-19
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training

Method used

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  • Molecular marker for identifying A10 and C07 chromosome segregation conditions of interspecific hybrids of Chinese cabbages and brassica carinata, and progeny materials of interspecific hybrids
  • Molecular marker for identifying A10 and C07 chromosome segregation conditions of interspecific hybrids of Chinese cabbages and brassica carinata, and progeny materials of interspecific hybrids
  • Molecular marker for identifying A10 and C07 chromosome segregation conditions of interspecific hybrids of Chinese cabbages and brassica carinata, and progeny materials of interspecific hybrids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 This example identifies the hybrid F between Chinese cabbage and Ethiopian mustard 1 plant

[0041] 1.1 Extract the F to be detected 1 Genomic DNA of the plant and its parents.

[0042] 1.2 Synthetic primers:

[0043] C07A2-F: 5'-TGCCCTCCAAAATCCAATTA-3' (SEQ ID No.1);

[0044] C07A2-R: 5'-CAGAAGCTCGGGAAGACATC-3' (SEQ ID No. 2).

[0045] 1.3PCR amplification. To be detected F 1 Plants and their parental DNA are used as templates, and the above primers are used for PCR amplification reaction. The reaction system is 10 μL, including: 1×PCR Buffer (containing Mg + ), 1ng template DNA, 0.2mM dNTPs, 0.5μM primer C07A2-F, 0.5μM primer C07A2-R, 1U Taq enzyme. PCR reaction conditions: 94°C for 3min; 94°C for 30s, 56.1°C for 30S, 72°C for 30S, 35 cycles; 72°C for 5min.

[0046] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, run electrophoresis at 180 volts for 1-2 hours, and stop electrophoresis ...

Embodiment 2

[0053] Example 2 This example identifies the inter-seed hybrid between Chinese cabbage and Ethiopian mustard and the backcross progeny of Ethiopian mustard (BC 1 )Material

[0054] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0055] 1.2 Synthetic primers:

[0056] C07A2-F: 5'-TGCCCTCCAAAATCCAATTA-3' (SEQ ID No.1);

[0057] C07A2-R: 5'-CAGAAGCTCGGGAAGACATC-3' (SEQ ID No. 2).

[0058] 1.3PCR amplification. Taking the plant to be detected and its parental DNA as a template, the PCR amplification reaction is carried out with the above primers. The reaction system is 15 μL, including: 1×PCR Buffer (containing Mg + ), 0.5ng template DNA, 0.2mM dNTPs, 0.5μM primer C07A2-F, 0.5μM primer C07A2-R, 1U Taq enzyme. PCR reaction conditions: 95°C for 3min; 95°C for 30s, 56.1°C for 30S, 72°C for 30S, 35 cycles; 72°C for 10min.

[0059] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, electrop...

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Abstract

The present invention discloses a molecular marker and a method for identifying interspecific hybrids of Chinese cabbages and brassica carinata and tracking A10 and C07 chromosome segregation conditions of progeny materials of the interspecific hybrids, and belongs to the field of plant genetic breeding. The marker is a pair of co-dominant molecular markers, used for identifying authenticity of the interspecific hybrids of the Chinese cabbages and brassica carinata, and also can be used for identifying and tracking the A10 and C07 chromosome segregation conditions of self-bred progeny, backcross progeny, chromosome additional lines of distant hybrids , recombinant segregation populations and plants.

Description

technical field [0001] The invention relates to the field of genetic breeding, in particular to a method for identification and selection of distant hybrid plants. Background technique [0002] Distant hybridization is an important means to create new plant germplasm and expand breeding resources. Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training. In the process of distant hybridization, false hybrid plants may be produced due to incomplete castration, female gametes developing into plants, and other reasons. Therefore, the plants obtained by distant hybridization need to be tested whether they are true hybrids by molecular, cytological and other methods. The offspring of self-crossing and backcrossing of distant hybrids need to track chromosomes or chromosome fragments by molecular or cytological meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 李锡香张晓辉宋江萍王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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