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Molecular markers for identification of interspecific hybrids of kale and red cabbage and tracking chromosome segregation of a05 and c04 in their progeny

A technology of molecular markers and chromosomes, applied in the field of genetic breeding, can solve time-consuming and labor-intensive problems, and achieve the effect of simple and fast cost, low technical requirements, and expansion of genetic resources

Active Publication Date: 2022-04-22
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training

Method used

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  • Molecular markers for identification of interspecific hybrids of kale and red cabbage and tracking chromosome segregation of a05 and c04 in their progeny
  • Molecular markers for identification of interspecific hybrids of kale and red cabbage and tracking chromosome segregation of a05 and c04 in their progeny
  • Molecular markers for identification of interspecific hybrids of kale and red cabbage and tracking chromosome segregation of a05 and c04 in their progeny

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 This example identifies Chinese kale and red cabbage moss interspecies hybrid F 1 plant

[0043] 1.1 Extract the F to be detected 1 Genomic DNA of the plant and its parents.

[0044] 1.2 Synthetic primers:

[0045] C04a-F: 5'-TCCGGAACTCATGAATGCTT-3' (SEQ ID No.1);

[0046] C04a-R: 5'-TTTTGGGTGCCTCGTGTAAT-3' (SEQ ID No. 2).

[0047] 1.3PCR amplification. To be detected F 1 Plants and their parental DNA are used as templates, and the above primers are used for PCR amplification reaction. The reaction system is 10 μL, including: 1×PCR Buffer (containing Mg 2+ ), 0.5ng template DNA, 0.2mM dNTPs, 0.5μM primer C04a-F, 0.5μM primer C04a-R, 1U Taq enzyme. PCR reaction conditions: 94°C for 3min; 94°C for 30s, 55.8°C for 30S, 72°C for 30S, 35 cycles; 72°C for 10min.

[0048] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, run electrophoresis at 180 volts for 1.5 hours, and end the electrophoresis ...

Embodiment 2

[0055] Example 2 This example identifies the interspecies hybrid backcross progeny of kale and beetroot (BC 1 )Material

[0056] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0057] Synthetic primers:

[0058] C04a-F: 5'-TCCGGAACTCATGAATGCTT-3' (SEQ ID No.1);

[0059] C04a-R: 5'-TTTTGGGTGCCTCGTGTAAT-3' (SEQ ID No. 2).

[0060] 1.3PCR amplification. Taking the plant to be detected and its parental DNA as a template, the PCR amplification reaction is carried out with the above primers. The reaction system is 20 μL, including: 1×PCR Buffer (containing Mg 2+ ), 1ng template DNA, 0.2mM dNTPs, 0.5μM primer C04a-F, 0.5μM primer C04a-R, 1U Taq enzyme. PCR reaction conditions: 95°C for 3min; 95°C for 30s, 55.8°C for 30S, 72°C for 30S, 35 cycles; 72°C for 5min.

[0061] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, electrophoresis at 180 volts for 2 hours, and end the electrophoresis ...

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Abstract

The invention provides a molecular marker and an identification method for identifying the chromosome segregation status of A05 and C04 of kale and beetroot interspecific hybrids and their progeny materials, belonging to the field of plant genetics and breeding. The invention provides a molecular marker and an identification method for identifying the chromosome segregation of A05 and C04 of kale and beetroot interspecific hybrids and their progeny materials. The marker is a pair of co-dominant molecular markers, and the marker is used to identify kale and red The true hybrids of vegetable moss interspecific hybrids can also be used to identify the self-progeny, backcross progeny, chromosome appendage lines, and recombination segregated populations and plants of distant hybrids.

Description

technical field [0001] The invention relates to the field of genetic breeding, in particular to a method for identification and selection of distant hybrid plants. Background technique [0002] Distant hybridization is an important means to create new plant germplasm and expand breeding resources. Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training. In the process of distant hybridization, false hybrid plants may be produced due to incomplete castration, female gametes developing into plants, and other reasons. Therefore, the plants obtained by distant hybridization need to be tested whether they are true hybrids by molecular, cytological and other methods. The offspring of self-crossing and backcrossing of distant hybrids need to track chromosomes or chromosome fragments by molecular or cytological meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 李锡香张晓辉宋江萍王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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