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Molecular markers and methods for identifying a06 and c05 chromosome segregation in Brassica vegetable interspecific hybrids and progeny materials

A chromosome and Brassica technology, applied in the field of genetic breeding, can solve time-consuming and labor-intensive problems, and achieve simple and fast cost, low technical requirements, and the effect of expanding genetic resources

Active Publication Date: 2022-05-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training

Method used

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  • Molecular markers and methods for identifying a06 and c05 chromosome segregation in Brassica vegetable interspecific hybrids and progeny materials
  • Molecular markers and methods for identifying a06 and c05 chromosome segregation in Brassica vegetable interspecific hybrids and progeny materials
  • Molecular markers and methods for identifying a06 and c05 chromosome segregation in Brassica vegetable interspecific hybrids and progeny materials

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1.3 PCR amplification. to be detected F

[0053] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis 1.5

[0054] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 1.

[0057] The F

Embodiment 2

[0064] 1.3 PCR amplification. to be detected F

[0065] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis 1.5

[0066] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 3.

Embodiment 3

[0072] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0076] 1.3 PCR amplification. Taking the plants to be detected and their parental DNAs as templates, PCR amplification reactions were carried out with the above primers. opposite

[0077] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis for 1 hour

[0078] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 5.

[0081] Whole genome illumina sequencing was carried out to the No. 9 plant after the identification of the molecular markers, and the sequencing depth was 10 ×;

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Abstract

The invention discloses a molecular marker and a method for identifying the segregation of A06 and C05 chromosomes of brassica vegetable interspecific hybrids and offspring materials, belonging to the field of plant genetics and breeding. The molecular marker can quickly and accurately identify the interspecific hybrids of Brassica A genome (such as Chinese cabbage, red cabbage, etc.) . The method can identify distant hybrids of Brassica A genome and C genome plants and their backcross offspring, which can expand the genetic resources of Brassica vegetables, transfer new traits, enrich vegetable types, and enrich people's daily dietary nutrition.

Description

Identification of chromosome segregation in Brassica vegetable interspecific hybrids and progeny materials A06 and C05 Molecular markers and methods for technical field [0001] The present invention relates to the field of genetics and breeding, in particular, to a method for identifying and breeding distant hybrid plants. Background technique [0002] Distant hybridization is an important means to create new plant germplasm and expand breeding resources. Due to reproductive isolation of species, distant Hybridization often requires techniques such as artificial pollination and embryo rescue, which are time-consuming and labor-intensive, and require certain scientific training. in the process of outbreeding , due to incomplete emasculation, the development of female gametes into plants, and other reasons may result in the production of pseudohybrid plants. thus, Plants obtained by distant hybridization need to be tested for true hybrids by molecular and cytological m...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 张晓辉李锡香宋江萍王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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