Method for preparing Rhodotorula benthica fermentation feed
A technology for marine red yeast and fermented feed, which is applied in animal feed, animal feed, application and other directions, can solve problems that have not yet been seen, and achieve the effects of low fermentation cost, improved nutritional value, and improved digestion and absorption rate.
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Embodiment 1
[0009] After crushing soybean meal, peanut meal and bran, mix the three evenly according to the weight ratio of 17:2:1 to make 1000g of soybean meal solid fermentation base material; in addition, 5g of yeast extract, KH 2 PO 4 0.2g, NaCl 1g, (NH 4 ) 2 SO 4 3.5g, 40g of sucrose and water are mixed to make 1000g nutritional supplement, and the pH value is adjusted to 6.0 with 1M HCl aqueous solution; the above-mentioned solid fermentation base material is mixed into the nutritional supplement, and the two are mixed evenly according to the weight ratio of 1:1.2, Make a solid fermentation medium, put it into a polyethylene plastic bag, tie the mouth of the bag tightly, cook at 100°C for 8 minutes, and cool.
[0010] When inoculating, first prepare Rhodotorula viscosus seed solution: first-level slant culture adopts potato medium, and after cultivating at 32°C for 24 hours, inoculate second-level marine yeast liquid medium, which consists of 2% glucose, 1% peptone, Yeast extra...
Embodiment 2
[0015] Crush soybean meal and peanut meal, and mix them evenly according to the weight ratio of 19:1 to make 1000g of soybean meal solid fermentation base material; in addition, 5g of yeast extract, KH 2 PO 4 0.1g, NaCl 1.5g, (NH 4 ) 2 SO 4 1.5g, 30g of sucrose and water are mixed to make 1000g nutritional supplement, and the pH value is adjusted to 6.0 with 1M HCl aqueous solution; the above-mentioned solid fermentation base material is mixed into the nutritional supplement, and the two are mixed evenly according to the weight ratio of 1:1.1, Prepare soybean meal solid fermentation medium, cook at 100°C for 8 minutes, and cool.
[0016] When inoculating, inoculate the seed solutions of Rhodotorula rubrum and Pediococcus lactis cultured to the logarithmic phase in the above-mentioned soybean meal solid fermentation medium at a ratio of 10% and 4%, respectively, and spread them on a tray with a thickness of 4cm after stirring evenly. Inside, cover tightly with a double-la...
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