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66 results about "Sorangium cellulosum" patented technology
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Sorangium cellulosum is a soil-dwelling Gram-negative bacterium of the group myxobacteria. It is motile and shows gliding motility. Under stressful conditions this motility, as in other myxobacteria, the cells congregate to form fruiting bodies and differentiate into myxospores. These congregating cells make isolation of pure culture and colony counts on agar medium difficult as the bacterium spread and colonies merge. It has an unusually-large genome of 13,033,779 base pairs, making it the largest bacterial genome sequenced to date by roughly 4 Mb.
Fermentation production method based on epothilone B metabolic pathways
InactiveCN103243134ARaise the level of fermentationReduce fermentation costsMicroorganism based processesFermentationBiotechnologyAlcohol
The invention discloses a fermentation production method based on epothilone B metabolic pathways and belongs to the technical field of fermentation engineering. The method comprises the following steps of: 1) inoculating Sorangium cellulosum into an M26 culture medium, carrying out shake cultivation to obtain a seed solution; 2) inoculating the seed solution into fermentation liquor containing resin, carrying out shake culturing for 72-120 hours at 25-35 DEG C, adding a precursor and small molecular substances into the fermentation liquor containing resin; and 3) filtering the fermentation liquor after the culture is finished, collecting the resin, washing the resin and oscillating and extracting with methyl alcohol to obtain extract liquor containing the epothilone B. According to the invention, the precursor and small molecular substances related to the biosynthetic pathways obtained by screening are added into the fermentation liquor, and the constructive metabolism pathways of the epothilone B are disturbed, so that the excellent bacterial strain gives full play to the ability of anabolism and the fermentation level of the epothilone B is improved greatly, thus the fermentation cost is reduced and the commercial process of the anti-cancer drug is promoted.
Owner:SHAANXI UNIV OF SCI & TECH
Stabilization of suspended solid particles and/or gas bubbles in aqueous fluids
This invention relates to compositions that have utility, amongst others, in the stabilization of suspension particles or gas bubbles in fluid water-based compositions and / or in conferring shear thinning behavior to such fluid water-based compositions. The inventors have developed parenchymal cellulose based materials, which comprise cell wall derived networks of cellulose based fibers and nanofibrils, can advantageously be used for stabilization of suspended solid particles in fluid water-based compositions. Specific aspects of the invention concern the parenchymal cellulose based materials, their production and their use in fluid water-based compositions, as well as the resulting fluid water-based compositions per se.
Owner:KONINK COOPERATIE COSUN U A
Xylose isomerase, and encoding gene and use thereof
The invention relates to a xylose isomerase and the coded protein and the application thereof, pertaining to the technical field of enzyme genetic engineering. A coded xylose isomerase DNA sequence is obtained from mutation at partial positions of a xylose isomerase gene xylA(Genebank EU643621) in Sorangium cellulosum 157-2. The invention also relates to enzyme coded by the DNA sequence and the application of the coded enzyme in the fields of chemical industry, food and medicine. In addition, compared with the traditional xylose isomerase, the activity of xylose isomerase of the invention in saccharomyces cerevisiae is increased by 2.8 times.
Owner:HENAN TIANGUAN GRP +1
Methods of obtaining epothilone D using crystallization and /or by the culture of cells in the presence of methyl oleate
InactiveUS6998256B2Useful in treatmentProduce epothilonesOrganic chemistryBacteriaMyxococcus xanthusMethyl oleate
Methods are provided for purification of epothilone D and epothilone D analogs from epothilone producing cells such as, for example, Sorangium cellulosum and Myxococcus xanthus (recombinant). Epothilone can be purified from fermentation broth, for example by using resins, chromatography, and crystallization. Epothilone D can be crystallized from a binary solvent system in which water is the forcing solvent.
Owner:KOSAN BIOSCI
A alkali-fast sorangium cellulosum and uses of the same in producing epothilone
The invention discloses an alkali proof Sorangium cellulosum strain named Sorangium cellulosum So0157-2, which is preserved in China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC No. M208078 on May 27, 2008. The invention also discloses the application of the alkali proof Sorangium cellulosum to the preparation of Epothilones. The Epothilones generated by the fermentation of the alkali proof Sorangium cellulosum has high yield stable fermenting property of strains and short fermentation period, is not easy to cause bacteria contaminants and does not generate Epothilones C, D, E, F and other homologues, thus greatly reducing the difficulty in subsequent separation and purification and being an Epothilones producing strain with high value in research and development.
Owner:SHANDONG UNIV
Kelp microcrystalline cellulose and preparation method thereof as well as application of prepared kelp microcrystalline cellulose
Owner:QINGDAO HENGSHENG BIOLOGICAL PHARMA TECH DEV
Fermentation process for raising ebomycin A yield
The present invention discloses a method for raising secondary metallic product output of myxobacteria, i,e, a method for raising output of metabolic product epothilone A of sorangium cellulosum of ephothilone in myxobacteria, and the said method incldues: 1). naturalization method of lump-grown myxobacterial liquid uniform growth; (2). selecting potato starch and hydrolyzed lactoprotein, etc. suitable for production of epothilone A; and 3). addition method of mixed resin for integrally-eliminating product metabolic accumulation so as to make the output of ephothiloue A product be up to 62.7 mg / L level.
Owner:SHANDONG UNIV
Method for highly-effective producing epothilone using myxobacteria sorangium cellulosum
InactiveCN1769468ASuitable for growthGrowth does not affectMicroorganism based processesFermentationMyxobacteriaEpothilone
The invention provides a process for producing Epothilones by utilizing Sorangium cellulosum, which comprises improving fermentation culture medium of Sorangium cellulosum, and charging cyclodextrins or cyclodextrin derivatives into the fermentation culture medium. The process can realize higher output of Epothilones production.
Owner:SOUTH CHINA UNIV OF TECH
Stabilization of suspended solid particles and/or gas bubbles in aqueous fluids
Owner:KONINK COOPERATIE COSUN U A
Fermentation method for producing epothilone B by sorangium cellulosum and fermentation medium
InactiveCN101851591AImprove performanceGrow fastBacteriaMicroorganism based processesInorganic saltsEpothilone B
The invention discloses a sorangium cellulosum fermentation medium, which comprises a carbon source, a nitrogen source, inorganic salts, a macroporous absorption resin, and water. The fermentation medium also comprises amino acid. When the fermentation medium is used for preparing the epothilone B by fermentation culture of sorangium cellulosum, the performance of strains is stable, the growth is rapid, and the yield of the product epothilone B is obviously improved. The invention also provides a fermentation method for producing the epothilone B by the fermentation culture of the sorangium cellulosum by using the sorangium cellulosum fermentation medium.
Owner:SHANGHAI INST OF PHARMA IND
Method for separation and extraction of epothilone B from sorangium cellulosum fermentation liquid
ActiveCN103667387APromote growthIncrease productionOrganic chemistryMicroorganism based processesDesorptionEthyl acetate
The invention provides a method for separation and extraction of epothilone B from a sorangium cellulosum fermentation liquid, and belongs to the microbial fermentation pharmacy field. The method comprises the steps: after shake-flask cultivation of sorangium cellulosum strains, amplifying step by step, fermenting sorangium cellulosum, followed by carrying out macroporous resin adsorption, ethyl acetate desorption and methanol redissolution, and thus obtaining a crude product; carrying out column chromatography and HPLC analysis of the crude product to obtain a preliminary component of epothilone B, then adding a molecularly imprinted polymer for specific adsorption of epothilone B, and finally carrying out methanol elution and crystallization to obtain epothilone B. Sorangium cellulosum is produced by step-by-step fermentation, and at the same time, the molecularly imprinted polymer with the specific targeted adsorption capacity is adopted in the separation and extraction process, not only has a strong adsorption capacity on epothilone B, but also can easily extract epothilone B, so as to reduce the extraction cost of separation and extraction of epothilone drugs, and have more wide industrialized application prospects and economic benefits.
Owner:SHAANXI UNIV OF SCI & TECH
Process for the preparation of free-flowing, pulverized atorvastatin adsorbates
InactiveUS20050106243A1Simpler and cheapImprove stabilityPowder deliveryBiocidePolyolAdditive ingredient
The invention relates to a method for the preparation of atorvastatin adsorbates and solvates thereof, wherein one starts from a solution comprising the pharmaceutical active pharmaceutical ingredient substantially dissolved therein, one suspenses an adsorber material therein selected from the group of the celluloses, cellulose derivatives, polyols, sugars, sugar derivatives, maltodextrins, cyclodextrins, starches, polydextroses or mixtures thereof, and one removes the solvent by drying. Also, the invention relates to atorvastatin adsorbates obtainable according to this method as well as pharmaceutical formulations comprising them.
Owner:HELM AG
Mango dreg biology feed and preparation method and application thereof
InactiveCN109699831AIncrease the fragranceImprove biological treatmentFood processingAnimal feeding stuffBiotechnologyMicroorganism
The invention belongs to the technical field of food processing, and particularly relates to a mango dreg biology feed and a preparation method and application thereof. The feed is prepared from the following raw materials in percentage by weight of 60%-75% of mango dregs, 8%-15% of apple dregs, 3%-8% of probiotics and 12%-25% of auxiliary materials. The preparation method comprises the followingsteps of weighing the raw materials, then pretreating mango dregs, mixing the pretreated mango dregs with auxiliary materials to obtain fermentation raw materials, and performing activating treatmenton microorganism bacteria; and then adding cellulase, performing anaerobic fermentation, and then performing aerobic fermentation so as to obtain the mango dreg biology feed. The mango dreg biology feed is applied to fattening and raising of live pigs. The mango dreg biology feed has the advantages that effective components of the mango dregs can be sufficiently decomposed, the utilization rate isincreased, and probiotics and the auxiliary materials can assist in promoting fermentation and can improve the quality of the feed. The feed can effectively increase the weight of the live pigs whenbeing used for raising the live pigs during fattening period.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
Preparation method of beef black bean sauce
InactiveCN106616370AImprove emulsion stabilityExtended shelf performanceFood scienceContinuous fermentationAcid value
The invention belongs to the technical field of food processing, and particularly relates to a preparation method of beef black bean sauce. The preparation method comprises the following steps: cleaning soybeans, spraying saturated water vapor to the surfaces of the soybeans at the temperature of 60 to 70 DEG C for treating, uniformly mixing the soybeans with aspergillus niger for fermentation, after fermentation is ended, mixing a mixture with sorangium cellulosum for fermentation again, cleaning beef, carrying out pelletizing, mixing the beef with monascus for fermentation, after the fermentation is ended, inoculating morel strains for continuous fermentation, carrying out infrared drying step by step on the fermented beef, carrying out mixed quick freezing on the fermented beef and the soybeans according to a mass ratio of 1 to 2, then heating and frying, and finally adding beta-lactoglobulin and hydrochloric stachydrine. The finished beef black bean sauce is bronzing; beef is soft and easy to chew, and the black beans are flexible and chewy; the acid value (KOH) is less than 4.3 mg / g; the beef black bean sauce can be stored for 10 months at normal temperature.
Owner:ANHUI WEIXIAN FOOD
Genes for the biosynthesis of epothilones
Owner:NOVARTIS AG
Method for improving sorangium cellulosum's epothilone B yield by genetic engineering
InactiveCN103834707AIncrease productionMicroorganism based processesFermentationBiotechnologyEpothilone B
The invention discloses a method for improving sorangium cellulosum's epothilone B yield by genetic engineering. The method includes: introducing a Vitreoscilla hemoglobin gene vgb into an expression vector PBEP43 to obtain a recombinant plasmid PBEP43-vgb, and then subjecting the recombinant plasmid PBEP43-vgb to electrotransformation into sorangium cellulosum So ce M4, thus obtaining a sorangium cellulosum So ce M4 recombinant strain, and realizing improving the sorangium cellulosum So ce M4's epothilone B yield. According to the invention, the electrotransformation method is employed to transform the gene of sorangium cellulosum So ce M4, thereby greatly improving the yield of epothilone B. The method provided by the invention has broad application prospects, and research in the aspect is not reported to date.
Owner:GUANGDONG INST OF MICROORGANISM
Method for increasing fermentation yield of epothilone by using competitive microorganism and application thereof
InactiveCN102399848AImprove fermentation yieldIncrease the amount of bacteriaMicroorganism based processesFermentationMicroorganismPenicillium bilaiae
The invention discloses a method for increasing the fermentation yield of epothilone by using the metabolin of competitive microorganism namely rhizopus arrhizus or penicillium decumbens of sorangium cellulosum to induce. The method provided by the invention comprises the following steps of: cultivating the competitive microorganism, and extracting the metabolin of the competitive microorganism; adding the metabolin into a sorangium cellulosum fermentation medium, activating epothilone to produce related regulator genes inside the strain by using the stimulation of the external additive, and further stimulating the synthesis of epothilone. Detection results show that the fermentation yield of epothilone can be increased by 46.3-58.7% as compared with original conditions after the method is used, and the method has important theoretical significance and economic value.
Owner:QILU UNIV OF TECH
Method for producing fibrous cellulose, and fibrous cellulose
ActiveUS20190127915A1Efficiently obtainedHigh viscosityPaper/cardboardChemical/chemomechanical pulpPhosphoric acidCellulose fiber
It is an object of the present invention to provide a method for producing ultrafine fibrous cellulose, which is capable of efficiently obtaining ultrafine fibrous cellulose having phosphoric acid groups with a high yield. The present invention relates to a method for producing fibrous cellulose having a fiber width of 1000 nm or less, comprising: a (A) of introducing phosphoric acid groups into cellulose fibers to form crosslinked structures via the phosphoric acid groups, thereby obtaining crosslinked phosphorylated cellulose fibers, a (B) of breaking some or all of the crosslinked structures to obtain crosslink-broken phosphorylated cellulose fibers, and a (C) of performing a mechanical treatment on the crosslink-broken phosphorylated cellulose fibers to obtain fibrous cellulose having a fiber width of 1000 nm or less, wherein, in the (A), crosslinked structures in an amount of 0.05 mmol / g or more and 2.0 mmol / g or less are formed, and the (B) is a step of performing the hydrolysis of the crosslinked structures in an aqueous solvent with pH 3 or more.
Owner:OJI HLDG CORP
Process for the preparation of adsorbates of valsartan and/or its solvates or hydrates
Owner:GLAENZER KLAUS
A method based on porous ceramic adsorption and immobilization of S. cellulosus fermentation
InactiveCN103045675BHelps relieve feedback inhibitionReduce manufacturing costMicroorganism based processesFermentationFiberMicroorganism
The invention discloses a method for absorbing and fixing sorangium cellulosum for fermentation based on porous ceramics and belongs to the field of microbial fermentation pharmacy. The method comprises the steps that the prepared porous ceramics are processed by fermentation liquid to be dried for stand-by application; after sorangium cellulosum is cultivated through an M26 solid and a liquid medium respectively, bacterium suspension is formed; and after dilution, the bacterium suspension is inoculated in the fermentation liquid, the processed porous ceramics are added into the fermentation liquid, and shake cultivation is performed. The method for absorbing and fixing an epothilone production bacterial strain based on kieselguhr-based porous ceramics is provided by the invention, when epothilone B is in the fermentation production, sorangium cellulosum, which is the production bacterial strain of the epothilone B, is fixed, so that a solid attachment surface can be provided for a slime bacteria cell growing in a liquid environment, the method has the advantages that the fermentation production yield can be increased, the production cost of epothilone which is an anti-tumor medicine is lowered, and the operation steps are simple and easy to implement, therefore, the method can be amplified to the industrial production and establishes an experimental basis for the industrial production of epothilone.
Owner:SHAANXI UNIV OF SCI & TECH
Genes encoding the synthetic pathway for the production of disorazole
The present invention relates to nucleic acid sequences and proteins derivable therefrom that have been identified in Sorangium cellulosum, which proteins are catalytically active or participate in the biosynthetic pathway of disorazoles. The invention provides novel sequences which are necessary components of the disorazole biosynthetic pathway in addition to genes dszA-D.
Owner:GESELLSCHAFT FUR BIOTECHNOLOGISCHE FORSCHUNG MBH GBF
Transformation method of two sorangium cellulosum strains
InactiveCN103820376AIncrease abundanceIncrease productionBacteriaVector-based foreign material introductionPlasmid VectorShuttle plasmid
The invention discloses a transformation method of two sorangium cellulosum strains. In consideration of the condition that the available plasmid vectors in the conventional sorangium cellulosum transformation method are relatively less, exogenous genes (vgb) are respectively led into the two sorangium cellulosum strains by anti-double-stranded shuttle plasmid vectors and anti-double-stranded wide host vectors. A foundation is laid for the establishment of a genetic operation system of the sorangium cellulosum and the promotion of the gene engineering modification of the sorangium cellulosum, so that the abundance and the yield of active secondary metabolites in the sorangium cellulosum are improved.
Owner:GUANGDONG INST OF MICROORGANISM
Conversion method for two fiber sorangium cellulosum strains
InactiveCN105062940AIncrease abundanceIncrease productionBacteriaVector-based foreign material introductionFiberPlasmid Vector
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Tissue with nanofibrillar cellulose surface layer
Owner:GPCP IP HLDG LLC
Method for treating uranium enrichment biomass through combination of phanerodontia chrysosporium and modified activated carbon and application thereof
ActiveCN108486393AEffective leachingAchieve reductionFungiBacteriaThiobacillus ferrooxidansActivated carbon
The invention discloses a method for treating uranium enrichment biomass through combination of phanerodontia chrysosporium and modified activated carbon and application thereof. The purpose is to solve the problem that currently, corresponding uranium enrichment biomass pre-treatment methods are scarce so that the requirements of uranium enrichment biomass treatment cannot be met. According to the method, thiobacillus ferrooxidans are used for degrading the uranium enrichment biomass lolium perenne, the phanerodontia chrysosporium is used for secondary degradation of residues, and finally, the activated carbon is used for conducting adsorption treatment on uranium generated by degradation to achieve enrichment treatment of the uranium in the uranium enrichment biomass. Furthermore, according to the method, through orthogonal experiment optimization, the material dosages in the technology are greatly reduced, and the cost and the energy consumption in the technology are reduced, whichis more beneficial to application and development of the industry of the method; the phenomenon that the method has a better degradation effect on lignin, hemicellulose and cellulose in the uranium enrichment biomass, higher adsorption efficiency on the uranium, and important significance to reduction and harmlessness treatment conducted on the uranium enrichment biomass is verified.
Owner:四川戈润恩生态科技有限公司
Novel TALEN carrier suitable for sorangium cellulosum and construction method of novel TALEN carrier
InactiveCN104673820AFermentationVector-based foreign material introductionEscherichia coliWestern blot
The invention discloses a novel TALEN carrier suitable for sorangium cellulosum and a construction method of the novel TALEN carrier. The construction method comprises the following steps: firstly, replacing a promoter suitable for eucaryon with a promoter P43 suitable for escherichia coli and bacillus subtitles; and transferring TALEN elements of a left arm and a right arm of the successfully constructed target gene and the target gen into the sorangium cellulosum, and validating whether the recombinant protein is expressed with Western blot, so as to validate that the constructed carrier can perform the function of knocking out the gene in the sorangium cellulosum. The application range of a TALEN technique is further expanded; and the development of a biotechnology is promoted.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Sorangium cellulosum, preparation method and application for Phoxalone fermented by the same
A sorangia cellulosum and a method to prepare Phoxalone from the fermentation of the Sorangia cellulosum and the applications thereof pertain to the technical field of bio-engineering and natural medicine. The invention relates to a strain of high active anti-cancer substance - Sorangium cellulosum MWXAB-125 (So ce MWXAB-125), so as to produce Phoxalone and derivatives and analogs of Phoxalone from the fermentation of Sorangium cellulosum; the invention also relates to the preparation methods thereof, the identifications of substance structures thereof and the extrinsic anti-tumor activities as cell inhibitors.
Owner:JIANGNAN UNIV
Fermentation additive capable of changing generation rate of epothilone compound and improving yield of epothilone A
ActiveCN105200093AChange generation ratioGood water solubilityMicroorganism based processesFermentationGeneration rateEpothilone B
The invention discloses a fermentation additive capable of changing the generation rate of an epothilone compound and improving the yield of epothilone A. The additive is 2,3,5,6-tetrahydroxy-2-hydrosorbic acid-4-lactone; and the final adding concentration in an epothilone liquid fermentation culture medium is 100+ / -15 mu mol / L. An experiment proves that the additive disclosed by the invention is capable of keeping the reducibility of metal ions and metabolic intermediates and adjusting the secondary metabolism flow direction of sorangium cellulosum, so that the gross of the epothilone A is averagely improved by 62%-105% in comparison with that when the additive is not added; the ratio of the epothilone A to the epothilone B in a fermentation liquid is significantly changed; and (1.83+ / -0.16):1 (the epothilone A to the epothilone B) before addition is changed into (5.67+ / -0.71):1 (the epothilone A to the epothilone B). According to the fermentation additive, regulation and control on the secondary metabolism flow direction and the product type of the epothilone compound in the fermentation process are achieved; the fermentation cost of the epothilone A is reduced; and a way is also provided for production of the epothilone A.
Owner:SHANDONG UNIV
Method for the Genetic Modulation of the Biosynthesis of Hemicelluloses, Cellulose and Uronic Acids in Plant Cells Using Gene Expression Cassettes
InactiveUS20080277080A1Change outputImproves wood qualityNon-fibrous pulp additionHydrolasesPlant cellUronic acid
The present invention refers to gene expression cassettes encoding enzymes involved in the metabolic pathway for the biosynthesis of hemicelluloses, cellulose and / or uronic acids, and to a method for genetic transformation of plant cells through the introduction of one or more gene expression cassettes from the present invention, and the overexpression and repression of these genes in plants.More particularly, the present invention refers to a method for introducing expression cassettes in plants. Additionally, the present invention refers to genetically modified plants.The present invention also refers to the attainment of the genetically modified plant, its derived plants and seeds, as well as the wood, paper and cellulose from this plant.
Owner:SUZANO PAPEL E CELULOSE +2
Method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms
ActiveCN111269042AOvercome Difficult Growth ProblemsSuccessful colonizationBio-organic fraction processingExcrement fertilisersBiotechnologyMycelium
The invention discloses a method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms. The method includes the steps of: inoculating pleurotus ostreatus mycelium into straw debris for pre-culture, and then spreading the pre-cultured straw debris on the primary fermentation product of the livestock and poultry manure to form a fermentation substrate; fermenting the fermentation substrate for the second time; or paving the straw fragments on the primary fermentation product of the livestock and poultry manure, and inoculating oyster mushroom mycelia on the straw fragments to obtain a fermentation substrate; and fermenting the fermentation substrate for the second time. The method has the beneficial effects: (1) straw cell walls are damaged and hemicellulose-cellulose is dissolved out after corn straw chippings are subjected to oyster mushroom pretreatment, so that a basis is provided for functional bacteria to convert hemicellulose-cellulose into fulvic acid after the functional bacteria are started; (2) the oyster mushrooms are pre-cultured, and the problem that the oyster mushrooms are difficult to grow in a high-nitrogen substrateis solved through an interlayer covering and adding means, so that the oyster mushrooms are smoothly colonized in a pile body;
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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