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Method for highly-effective producing epothilone using myxobacteria sorangium cellulosum

A technology of Cystobacter cellulis and epothilone, applied in the field of microorganisms, can solve the problems of hair loss and allergic reactions, difficulty in extracting Epothiliones, strong cytotoxicity of multidrug-resistant cells, etc., and achieve the effect of weakening product inhibition and increasing yield.

Inactive Publication Date: 2006-05-10
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

[0009] 3. Epothilones are not substrates of P2 glycoprotein and have strong cytotoxicity to multidrug resistant cells
[0010] 4. The administration of paclitaxel will be accompanied by some clinical side effects (such as neutropenia, peripheral neuropathy, alopecia and allergic reactions)
Therefore, in actual large-scale applications, the method of adding resin cannot be used.
However, if no resin is added, it is difficult to extract a higher concentration of Epothiliones in the fermentation medium
[0013] So far, the method of obtaining Epothiliones from myxobacteria and applying it to the field of industrial technology production without the above-mentioned disadvantages is still in the seeking stage

Method used

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  • Method for highly-effective producing epothilone using myxobacteria sorangium cellulosum

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Embodiment

[0050] 1. Activation of strains: Take out S. cellulosus DSM11999 from the -80°C refrigerator, first place the strains in ice water to slowly heat up and dissolve, take 1ml and transfer to 10ml of G52 medium (put in a 50ml shake flask) Cultivate for 3 days, 180 rpm, 30°C, shake flask displacement 25mm. Then take 5 ml of this G52 culture and transfer it to 45 ml of G52 medium (put in a 200 ml shake flask) and cultivate for 3 days, 180 rpm, 30° C., and the shake flask is displaced by 25 mm. Then take 50 ml of G52 culture and transfer it to 450 ml of G52 medium (in a 2L shake flask) for 3 days, 180 rpm, 30° C., and the displacement of the shake flask is 50 mm. Medium G52 is: dry yeast powder 2g / L, MgSO 4 1g / L, CaCl 2 1g / L, skimmed milk powder 2g / L, potato starch 8g / L, anhydrous glucose 2g / L, EDTA-Fe(III)-Na 1ml / L, adjust the pH to 7.4, and sterilize at 120°C for 20 minutes;

[0051] 2. Maintenance culture: Take 50 ml of the above-mentioned culture and add it to 450 ml of G52 m...

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Abstract

The invention provides a process for producing Epothilones by utilizing Sorangium cellulosum, which comprises improving fermentation culture medium of Sorangium cellulosum, and charging cyclodextrins or cyclodextrin derivatives into the fermentation culture medium. The process can realize higher output of Epothilones production.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a high-efficiency production method of epothilone. Background technique [0002] Myxobacteria (Myxobacteria) are the highest Gram-negative prokaryotic group, with complex multicellular behavior, occupying an important position in the study of cell differentiation, development and biological evolution. Myxobacteria can produce extremely rich secondary metabolites, and the diversity of biological activity of the products can be compared with the famous Streptomyces group, which is an excellent strain resource in the development and research of new microbial drugs. [0003] Sorangium cellulosum belongs to the cellulolytic group of myxobacteria, and the macrocyclic ester compound epothilone (epothilone) produced by it has the activity of promoting microtubule polymerization. carcinogen. Epothilone can combine with the microtubule skeleton in eukaryotic cells, resulting in blocked chro...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/01
Inventor 罗立新陆一鸣汪薇潘力
Owner SOUTH CHINA UNIV OF TECH
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