107results about How to "The test results are consistent" patented technology

The invention belongs to the field of the evaluation of reservoir rock physical properties, and particularly relates to a testing device for the gas permeability of compacted sandstone. The testing device comprises a high pressure gas cylinder, a buffer container, a core holding unit, a back-pressure valve, a confining pressure pump and a return pressure pump, wherein the high pressure gas cylinder is provided with a reducing valve, the buffer container is connected with the reducing valve through a first pipeline, and the buffer container is connected with a vacuum pump through a second pipeline; the inlet of the core holding unit is connected with the buffer container through an inlet pipeline, the outlet of the core holding unit is connected with the inlet of the return pressure pump through an outlet pipeline, and the return pressure valve is communicated with atmosphere; a pressurization connector of the return pressure valve is connected with the return pressure pump through a third pipeline, and a confining pressure connector of the core holding device is connected with the confining pressure pump through a fourth pipeline. The testing device for the gas permeability of the compacted sandstone provided by the invention is simple to operate, a theory is likely to be deduced, the klinkenberg permeability and a slip factor can be obtained at the same time by a testing pressure depression curve only; furthermore, compared with a steady-state method, the testing device is accurate in result and takes short time.

The invention discloses a high-reliability pressure-adjusting automatic testing apparatus for a fingerprint module group. The automatic testing apparatus comprises a pedestal, a left support plate, a right support plate, a horizontal bridge plate, at least one horizontal objective table and a switching board installed on the horizontal objective table. The horizontal bridge plate is fixed between the respective upper ends of the left support plate and the right support plate. At least one lifting plate is arranged between the horizontal bridge plate and the horizontal bridge plate; and an inverted-T-shaped through hole is formed in the upper end of the lifting plate and is formed by a penetrating connecting hole and a rectangular through hole. A hanging plate is installed on the front end surface of the lifting plate; a plastic cavity box is installed at the lower end surface of the hanging plate; and a left cylinder, a right cylinder, and a testing pressing head seta are arranged in the cavity of the plastic cavity box respectively; and a testing pressing head for detecting an electrical performance of the fingerprint module group is fixed at the testing pressing head. According to the invention, the force applied on a product force can be adjusted and controlled; and products for testing are diversified. After testing by multiple times, the testing apparatus with the pressure applying tool can have a stable testing result; and testing results are consistent.

The invention relates to a livestock meat quality portable lossless detector based on portable communication equipment, which comprises a microcontroller unit capable of interacting data with the portable communication equipment through a wired or wireless mode, a light source unit capable of providing stable illumination for a data information acquisition unit, a microcontroller unit respectively connected with the data information acquisition unit and a positioning sensing unit, wherein the microcontroller unit is capable of performing real-time reception of the positioning information transmitted by the positioning sensing unit. When the received positioning information is consistent with the preset positioning information, the user presses a starting button to control the data information on the surface of the meat to be detected which is acquired by the data information acquisition unit, and then the data information is sent to the microcontroller unit, then is transmitted to the portable communication equipment by the microcontroller unit, and finally, the data information is sent to an assigned communication server by the portable communication equipment for analysis and treatment. The portable lossless detector has the advantages of simple structure, low cost, light weight, easy carrying, fast detection speed, no destroy on the sample, high universality, and convenient popularization and application.

The invention discloses a ballastless track board disengaging nondestructive detection method based on elastic waves. By means of confirming a function relationship between shock excitation elastic wave frequency and disengaging surface elastic wave reflectance, a function relationship between the shock excitation elastic wave frequency and packing layer elastic wave reflectance is confirmed, the shock excitation elastic wave frequency which can make the ratio of the disengaging surface elastic wave reflectance to the packing layer elastic wave reflectance obtain the maximum value is found, shock excitation elastic wave with the frequency is directly stimulated, so that a disengaging surface reflected signal is in a state easiest to identify, and the disengaging judgment accuracy of a ballastless track board is greatly improved. On the basis, a judging standard whether the disengaging exits is provided at the same time. The invention further discloses a ballastless track board disengaging rate detection method based on the elastic waves.

The invention discloses botryosphaeria dothidea LAMP (Loop-mediated Isothermal Amplification) detection primers and application thereof. A primer composition for detecting botryosphaeria dothidea based on an LAMP technology consists of 6 specific primers, namely F3, B3, FIP, BIP, LF and LB. The invention further provides a botryosphaeria dothidea LAMP detection method. The botryosphaeria dothideaLAMP detection method comprises the following steps: 1), extracting DNA of a sample to be detected; 2), performing loop-mediated isothermal amplification on the DNA of the sample to be detected by using the primer composition provided by the invention; 3), performing result detection, wherein when a chromogenic result is green fluorescence or a trapezoidal electrophoretic band appears, a positiveresult is determined, and when a chromogenic result is orange or orange yellow or no trapezoidal electrophoretic band appears, a negative result is determined. A novel molecular detection method and the novel primer composition are provided for the botryosphaeria dothidea, the botryosphaeria dothidea can be detected quickly, conveniently and efficiently at high specificity and high sensitivity, and a reliable technological and theoretical basis is provided for early diagnosis, detection and rapid import and export inspection of the botryosphaeria dothidea.

The invention discloses a method and device for real-time adjustment of lens installation flatness. The camera real-time adjusting method comprises the steps of acquiring images which are shot by a camera and correspond to at least two groups of test charts in different positions, pre-processing the images to obtain each group of test chart through separation, calculating and displaying the real-time resolution value, relative to each group of test chart, of the light-sensitive surface of the camera according to each group of test chart in real time, and adjusting the installation angle of the optical axis of a lens of the camera relative to the light-sensitive surface in real time according to the real-time resolution value, relative to each group of test chart, of the light-sensitive surface of the camera. By means of the method, the real-time resolution value of the camera can be obtained through automatic real-time testing, a measurement result consists with a visual evaluation result obtained through visual inspection, flexibility and adjustability are realized, robustness is high, and the installation angle of the optical axis of the lens of the camera can be adjusted according to the real-time resolution value of the camera.

The invention discloses a method for manufacturing an immunosensor for detecting chlorpyrifos, which belongs to the technical field of biologic sensor manufacturing. The method comprises the following steps: dropwise coating the surface of a naked glassy carbon electrode, which is subjected to cleaning, activation and performance testing, with a nickel aluminum hydrotalcite-graphene compound liquid, subsequently modifying by hollow nanogold, further fixing a chlorpyrifos monoclonal antibody on the modified electrode, and finally, sealing by bovine serum albumin. The immunosensor manufactured by the method has the characteristics of high sensitivity, low cost, high detection speed, good stability and high sample recycling rate. The detection limit of the immunosensor manufactured by the method can be 0.052ng / L, the linear range of the immunosensor is 5ng / mL-2 micrograms / mL, the recycling rate of the immunosensor can be 82.0-118.0%, and therefore the immunosensor is applicable to rapid detection on the residual amount of chlorpyrifos in fruits and vegetables.

The invention discloses a hybrid interlayer crack propagation analysis method for a composite laminated plate, and relates to the field of composite material laminated plate interlayer failure, the analysis method comprises the following steps: 1, establishing a laminated plate model containing general layered cracks; 2, a single DCB test piece crack propagation analysis model is established; 3, establishing a single ENF test piece crack propagation analysis model; and 4, establishing a crack propagation analysis model of the mixed crack MMB test piece; the method disclosed by the invention issuitable for analyzing the crack propagation process of the composite material laminated plate test piece in a single and mixed layered fracture mode; rigid body rotation around a simply supported constraint point at the right end of a test piece and a softening behavior of a crack tip are simultaneously considered in a mixed fracture mode, and the method can accurately and reliably predict a general mixed layered crack propagation behavior, is suitable for engineering practice, and provides a reliable cross inspection tool for numerical simulation at the same time.

The invention relates to a solid phase filter membrane detection method of sperm acrosin activity. The method comprises steps that: sperms are absorbed on the surface of the solid phase filter membrane; liquid and impurities other than sperms are removed from the surface of the solid phase filter membrane by using a centrifugation method or a dumping method; an acrosin activity detection reactionis started, wherein the sperms absorbed on the surface of the solid phase filter membrane contact a substrate solution, such that the acrosin activity detection reaction is started; the reaction is carried out for 30 to 120 minutes under a temperature of 4 to 40 DEG C; the substrate is separated from the sperms on the solid phase, or a quality control stop buffer is added in; reaction products are processed through colorimetry; and acrosin activity is obtained by calculating the absorbance values obtained through colorimetry. The invention also relates to a quantitative detection kit for sperm acrosin activity. According to the invention, a special-purposed reaction apparatus is adopted, the operation is simple, the result is accurate, and the detection means is flexible. The method and the kit are suitable for batch detections, and are easy to popularize.

The invented kit provides features of fast diagnosis and detection, sensitivity, easy of operation without need of professional personnel, and on-site measurement. Bacteriostasis test for extract of Chinese traditional medicine or hydrophobicity medication shows that effect of the invented kit is better than effect of traditional paper strip method. The kit contains reagents of MTT and tetrazole triphenyl chlorinated (TTC). In detecting mastitis of milk cow following steps are carried out: adding milk sample and 0.1ml reagents of MTT and TTC at room temperature for shortest acting time 4-5 minutes; adding soluble color development liquid, and observing result. Based on whether color is appeared, the result is determined.

The invention provides a rice fertility recovery gene auxiliary breeding molecular marker, which is SNP markers RSRF30121 and FRF4-10-01 respectively co-separated from rice fertility recovery genes Rf3 and Rf4. The marker RSRF30121 is used for detecting a 5606898-th site basic group of an No.1 rice chromosome; the marker FRF4-10-01 is used for detecting a 18838172-th site basic group of an No.10 rice chromosome; primer sequences of the marker RSRF30121 developed on the basis of KASP technology are shown as SEQ ID NO:1-3. Primary sequences of the marker FRF4-10-01 developed on the basis of the KASP technology are shown as SEQ ID NO:4-6. The invention also provides application of the SNP markers RSRF30121 and FRF4-10-01 to rice fertility recovery gene auxiliary breeding. By using the marker combination, whether the rice has the fertility recovery gene or not can be fast identified; important significance is realized on promoting the application of the Rf3 and Rf4 fertility recovery genes to commercial breeding.

The invention discloses a method for automatically testing a single board of a protective relay device. According to the method, a hook function is added in a program on a single board plug-in unit; and when a test program data packet is sent, the hook function can acquire the corresponding data packet and store the data packet in an executable RAM region. An application program can automatically jump to the RAM region to execute the test program and test whether the functions of the single board are normal or not; and after the single board is powered down and powered up again, the application program cleans up the RAM region data packet after self-checking the RAM, and the test program is automatically deleted. The method disclosed by the invention can bring conveniences for testing the single board of the protective relay device, reduce the single board debugging steps, and improve the production efficiency.

The invention discloses primers and a method for detecting alfalfa root rot fungi by virtue of loop-mediated isothermal amplification. The primers include a group of outer primers F3 / B3 and a group of inner primers FIP / BIP, wherein base sequences of the primers are shown as SEQ ID NO.1-4. The method comprises the following steps: extracting DNA of a to-be-detected sample; with the DNA as a template, conducting LAMP isothermal amplification by virtue of the primers; and adding a fluorescent dye to an LAMP amplification product for developing color, observing the color of the LAMP amplification product, and analyzing the product so as to judge whether the alfalfa root rot fungi exist or not. With the application of the primers and the rapid LAMP detection method thereof, the alfalfa root rot fungi can be rapidly, sensitively and accurately detected in production practice; meanwhile, the primers and the rapid LAMP detection method thereof are applicable to the early diagnosis of field diseases as well as the monitoring and identification of fungi; and in addition, the primers and the method are simple and easy to operate, and reliable technology and theoretical basis are provided for the control of alfalfa root rot.

The invention discloses an immune colloidal gold test stripe and preparation method and application, and the immune colloidal gold test stripe comprises a rigid polyvinyl chloride backer board, a nitrocellulose film, a sample mat, a colloidal gold combination mat and a water absorption mat, the nitrocellulose film is pasted to the rigid polyvinyl chloride backer board, the colloidal gold combination mat is pasted to one end of the nitrocellulose film, the sample mat is pasted on the colloidal gold combination mat, the water absorption mat is located at the other end of the nitrocellulose film; the preparation method comprises the steps as follows: (1) having reaction on the trisodium citrate and chloroauric acid for preparing colloidal gold; (2) marking the canine adenovirus resisting virus II type monoclonal antibody on the colloidal gold test stripe; (3) covering the canine adenovirus resisting virus II type antibody on the nitrocellulose film; (4) orderly pasting together for obtaining colloidal gold test stripe. The application of the immune colloidal gold test stripe for detecting canine adenovirus virus II type, the test stripe is simple in operation, fast and sensitive in detection, clear in result and easy in judgment. The immune colloidal gold test stripe is convenient to carry and use for saving the sickness detection cost.

The invention discloses a preparation method of an aptamer sensor for pesticide detection, and belongs to the technical field of safety detection of agricultural products. The preparation method provided by the invention comprises the following steps: drop-coating carbon black-chitosan compound liquid on a surface of a bare glassy carbon electrode after cleaning, activation and performance testing; then, modifying with a graphene oxide-iron oxide compound; immobilizing a pesticide aptamer on the modified electrode; and finally, closing with bovine serum albumin. The aptamer sensor prepared in the invention has characteristics of high sensitivity, low cost, high detection speed, good stability and high sample recovery rate, has a detection limit of 0.033 ng / mL, a linear range of 0.1-105 ng / mL, a recovery rate of 96.0-106.0%, and is suitable for rapid detection of pesticide residues in fruits and vegetables.

The invention discloses a novel glucose sensor free from interference of xylose. The sensor, based on regulation of formulation, is free from influence of a xylose interference factor on the basis ofhaving the own advantages of dehydrogenase. The sensor comprises a substrate layer, an electrode layer located on the substrate layer, an insulating layer located on the electrode layer, a reagent layer covering the insulating layer, a hydrophilic septal composite layer located between the insulating layer and the reagent layer, and a shielding adhesive tape layer located on the hydrophilic septalcomposite layer, wherein the number of electrodes is reduced for the electrode layer through sharing of a packed cell volume counter electrode and a blood glucose test counter electrode, electrode structure is optimized, and the shielding adhesive tape layer is provided with a rectangular notch which is convenient for blood sample and quality control liquid test. The glucose sensor provided by the invention is optimized in formulation and rational in structure design, has less interference factors and is highly precise in test.

The invention discloses a detection method for pore sizes and pore size distribution of a diaphragm. The method comprises the following steps: a visible spectrophotometer is used for scanning the diaphragm in a range set from 300m to 900nm, and the visible spectrophotometer is capable of automatically scanning from the small wavelength to the maximum wavelength and generating a curve; seen from the curve, the light transmittance is about 47-49 at the 900nm of the wavelength to indicate that the average pore size of the diaphragm is 27mm and the pore sizes are distributed quite evenly; if the light transmittance is 41-42, the average pore size of the diaphragm is greater than 30mm and the pore sizes are distributed not evenly. The method is simple to operate, but the results are quite precise; no sample needs to be prepared and the detection time is short; no other chemical material and reagent are needed, and therefore, no potential chemical injury can be caused to the operator.

The invention belongs to the aircraft test flight technology, and particularly relates to a noise qualification approval equivalent test flight method of a large transport-category aircraft. The noise qualification approval equivalent test flight method of the large transport-category aircraft includes the steps of symmetrically setting three noise measurement points in the direction perpendicular to the center extension line of an airport ground track, carrying out an airworthiness noise flight test with a flight path plunge method, after the aircraft takes off, entering flight path plunge points to set the engine power, the flight height and the flight speed, carrying out the flight test according to a reference flight path, measuring noise when the aircraft reaches the preset height above the noise measurement points, going around to pull up the aircraft after noise measurement is completed, and repeatedly carrying out a next flight test, wherein in the whole test process, the aircraft keeps flying all the time. According to the noise qualification approval equivalent test flight method, the noise measurement points are greatly reduced, required noise test devices and required test persons are greatly reduced, cost is reduced, the test flight efficiency is improved, the test flight cycle is greatly shortened, the service life of an engine is prolonged, a test site is flexibly selected, and flight tasks of aircrafts in other types in the site can not be influenced.

The invention discloses a simulated grounding test system and method for a grounding material. The system comprises a simulated groove, a ground net and a power supply; the ground net is arranged in the simulated groove in a preset buried depth, the simulated groove is filled with sandy soil, and the power supply is connected to the ground net via a lead; and the simulated groove is formed by excavation in the flat ground. On the basis of an electromagnetic field theory and a principle of similitude, proportional relations among different related quantities during simulated test are derived, and large test is converted into small simulated test equivalently. The system and method can be used to carry out a series of simulated tests including grounding resistance measurement, new grounding material test, resistance reducing agent test and grounding characteristic research on the large ground net in a limited area directly, it can be ensured that a test effect is consistent with that of a real test; and a test place is small, the soil resistivity can be changed flexibly, various types of test can be completed, and the system and method can be applied to power frequency grounding simulated test, impact grounding simulated test, grounding body long-acting corrosion resistance test and the like.

The invention relates to a fluorescent probe for detecting beta-galactosidase as well as a preparation method and application of the fluorescent probe. The chemical structural formula of the fluorescent probe is shown as (I). The fluorescent probe provided by the invention is high in sensitivity, low in detection limit (0.168 mU / mL), good in selectivity, quick in response and capable of specifically detecting the activity and content of beta-galactosidase. A fluorescent method for screening a beta-galactosidase inhibitor is established for the first time on the basis of the fluorescent probe,the test result is consistent with the test result of a commercial substrate, and the method is reliable.

The invention discloses a polyadenosine diphosphate ribose polymerase-1 (PARP-1) single particle detection method based on dark field scattering imaging, and further discloses a label-free kit for identifying cancer cells. Quantitative detection of PARP-1 activity and dark field imaging of PARP-1 in cells on a single particle level are realized based on change of scattering spectral displacement of nanoparticles, so that cancer cells and normal cells are remarkably distinguished. White light is used as a light source to be combined with a dark field microscope and a single particle scatteringspectrometer, dark field imaging and scattered spectrum collection of a single plasma nanoparticle can be achieved, and compared with a traditional average measuring means, the method has higher detection sensitivity. The kit provided by the invention has the advantages of no labeling, high detection sensitivity, high spatial resolution and the like.

The invention discloses a multi-axis fatigue test method for a full-size structural member of an aircraft, and relates to the field of fatigue life tests for structural members of aircrafts. The method comprises the following steps: (1) obtaining a stress concentration part of the structural member by finite element simulation calculation; (2) sticking strain gauges on the part or around the stress concentration part; (3) recording actual measurement spectrums of each strain gauge and each strain rosette, and filtering to obtain a test strain spectrum; (4) analyzing the stress of the aircraftstructural member, simplifying the external force, and developing a test bed according to the simplified external force; (5) converting the actually measured strain spectrum into a multidirectional external force load spectrum of the test bed by using finite element optimization calculation; and (6) loading a rising and falling load spectrum, comparing whether the relative error between the strainvalue of the load spectrum and the actually measured strain spectrum on the aircraft is less than 10%, if so, carrying out a fatigue life test, and otherwise, returning to the step (4) to carry out stress analysis again. Test results show that the method can well reproduce the multidirectional stress condition of the aircraft structural member in actual use.

The invention discloses a detection method of insulator surface water content based on hyperspectral technology. The detection method comprises the following steps: shooting insulator surfaces with known different surface water contents by utilizing a hyperspectral imager so as to obtain a corresponding hyperspectral image, performing black-white correction, multiplicative scatter correction and smooth denoising on the hyperspectral image to obtain a hyperspectral line Xi of the surface water content Si, and then obtaining a water content BP neural network prediction model; shooting to obtaina hyperspectral image of a to-be-detected insulator, performing black-white correction, multiplicative scatter correction and smooth denoising to obtain the hyperspectral line X0 of the to-be-detectedinsulator, inputting the hyperspectral line X0 of the to-be-detected insulator into a prediction model to obtain the surface content of the to-be-detected insulator. Through the method disclosed by the invention, the non-contact real-time detection can be performed on the field insulator under an electrified condition, the operation is simple and the realization is simple; and the method can provide reliable and accurate test evidence for the dampproof and moisture-resistant design, manufacturing and maintenance of the insulator.

The invention discloses an African swine fever virus (ASFV) MGF-505R gene fluorescent PCR detection reagent and reagent kit, and an application of the reagent kit, and belongs to the field of virus detection. An MGF-505R gene is used as a reference, a pair of special PCR primers and a TaqMan fluorescent probe are designed and optimized, plasmids containing an MGF-505R gene sequence are used as positive control, and a standard curve is made. The analysis sensibility of the method can achieve 1.36 copies. When being used for detecting African swine fever viruses and identifying MGF-505R gene deletion vaccine strains and natural infection strains, the African swine fever virus MGF-505R gene fluorescent PCR detection reagent and reagent kit are high in susceptibility and high in specificity and suitable for fast detection.

The invention discloses a rapid, simple, specific and sensitive detection kit suitable for most laboratories for detecting Candida albicans based on RPA (recombinase polymerase amplification). The kitcomprises a pair of primers and a probe, the nucleotide sequences of the primers are shown as SEQIDNO.1-2, and the nucleotide sequence of the probe is shown as SEQIDNO.3. The kit has two forms: a real-time fluorescence detection kit and a test strip kit. Experiments show that the kit is short in candida albicans detection time and high in sensitivity and specificity, the cost is further saved bycombining a simple nucleic acid crude extraction method, a new technical reference can be provided for field and nursing point detection, and the kit is of great significance in the aspect of pathogenic bacteria detection in areas lack of resource basic equipment.

The invention provides a monoclonal antibody for a duck tembusu virus and application. A hybridoma cell strain 1-E11 with the collection number CCTCCNO being C2014219 and a hybridoma cell strain 4-C3 with the collection number CCTCCNO being C2014220 of the monoclonal antibody resisting the duck tembusu virus are obtained by using a purified duck-tembusu-virus immunized Balb / c mouse; a colloidal-gold test strip is prepared by utilizing the monoclonal antibody secreted by the cell strains, the 1-E11 is adopted as a labeled monoclonal antibody, 4-C3 is used as a coated monoclonal antibody, and the obtained test strip has the characteristics of fast and sensitive detection and good specificity. A result is clear and the judgment is easy; the carry and the use are convenient, and the detection cost for disease is saved.

The invention provides a medicolegal rapid detection kit based on 21 SNP (Single Nucleotide Polymorphism) mitochondrion loci and an application of the medicolegal rapid detection kit in individual recognition. The kit comprises an enriched primer mixture, a single-base extension primer mixture, amplification reagents, a nucleic acid purification column, an SNaPshot reaction mixed liquid, Hi-Di methanamide, GeneScan<TM>Size Standards-120LIZ, a positive control and a negative control, wherein the amplification reagents include dNTPs and rapid Taq polymerase, the positive control is DNA 9947A, and the negative control is ddH2O. The kit provided by the invention is low in cost, easy to operate, high in sensitivity, and wide in applicability, a detection result is accurate and can be acquired within 1.5-2 hours by virtue of a single-tube reaction, and precious time is saved for the individual recognition of human biological detection materials and the uncovering of cases.

The invention provides a primer probe combination for detecting the polymorphism of an MTHFR gene through fluorescent quantitative PCR. The primer probe combination comprises two groups of primers anddouble probes as shown in a sequence table; meanwhile, the invention provides a method for detecting the polymorphism of the MTHFR gene through fluorescent quantitative PCR. According to the invention, the polymorphism of the MTHFR gene is detected by adopting a real-time fluorescent quantitative PCR Taqman-MGB probe method, and a wild type and mutant type double-probe detection method is adopted, so specificity is improved, and cost is reduced; and a Taqman-MGB probe is superior to an ordinary probe in the aspects of accuracy, repeatability, specificity, sensitivity and the like of experimental results. Thus, the MTHFR gene polymorphism method established by the invention has the advantages of accurate result, high specific sensitivity, no toxicity, no pollution, low cost, rapidness, high efficiency and the like.

The invention relates to an extension measuring device after fracture of a sample. The device comprises a body, wherein the body is provided with a rectangular groove, the groove comprises a front wall, a back wall and a side wall, the groove is provided with a platen which can slide left and right, the front wall is provided with a blind hole, a spring is installed inside the blind hole, and one end of the spring is pressed on the platen. When the device is used, the to-be-inspected sample is put into the rectangular groove with fractures aligned, the spring compacts the platen to enable the fractures of the sample to mesh, a standard spaced line which is labeled on the sample in advance is measured through a digital readout vernier caliper and is compared with the distance between the standard spaced lines of the sample before the test, and the extension length of the sample is obtained. The device has the advantages of being convenient to use by using the spring to add stress, ensuring the consistent stress of every sample and obtaining a relatively consistent testing result.