Rice fertility recovery gene auxiliary breeding molecular marker and application thereof

Abstract

The invention provides a rice fertility recovery gene auxiliary breeding molecular marker, which is SNP markers RSRF30121 and FRF4-10-01 respectively co-separated from rice fertility recovery genes Rf3 and Rf4. The marker RSRF30121 is used for detecting a 5606898-th site basic group of an No.1 rice chromosome; the marker FRF4-10-01 is used for detecting a 18838172-th site basic group of an No.10 rice chromosome; primer sequences of the marker RSRF30121 developed on the basis of KASP technology are shown as SEQ ID NO:1-3. Primary sequences of the marker FRF4-10-01 developed on the basis of the KASP technology are shown as SEQ ID NO:4-6. The invention also provides application of the SNP markers RSRF30121 and FRF4-10-01 to rice fertility recovery gene auxiliary breeding. By using the marker combination, whether the rice has the fertility recovery gene or not can be fast identified; important significance is realized on promoting the application of the Rf3 and Rf4 fertility recovery genes to commercial breeding.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and crop breeding, in particular to a rice fertility restoration gene assisted breeding molecular marker and its application. Background technique [0002] Molecular marker-assisted selection breeding is the product of the combination of modern biotechnology and traditional breeding technology. It changes the breeding technology from traditional extensive and empirical to modern precision, making breeding more operable and predictable. . [0003] In the three-line hybrid rice breeding, according to the difference of the restoration relationship, there are three main types of cytoplasmic male sterility, wild defeat type (WA-CMS), Baotai type (BT-CMS) and red lotus type (HL-CMS) . Most researchers believe that wild-type cytoplasmic male sterility in rice is controlled by 2 pairs of recessive genes. Bharaj et al. (1995) believed that wild aborted male sterility is controlled by two pair...

AI Technical Summary

Problems solved by technology

[0004] For the identification of Rf3 and Rf4 restorer genes, the marker types mainly used in existing reports are SSR and InDel markers, which have the disadvantages of low polymorphism rate and small differences in breeding

The use of EB or polyacrylamide in the detection process will easily pollute the environment and cause harm to the human body

However, the main identification method used in traditional breeding is based on phenotypic observation and identification such as seed setting rate, which takes a long time and is easily restricted by environmental conditions. There are errors in identification results and low selection efficiency.

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