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Immune colloidal gold test stripe and preparation method and application

A colloidal gold test paper, colloidal gold technology, applied in the field of preparation of immune colloidal gold test strips in the detection of canine adenovirus type Ⅱ, immune colloidal gold test strips, can solve the cumbersome process of virus isolation and identification and neutralization test, molecular Biological methods have high requirements and electron microscope technology is difficult, etc., to achieve the effect of reasonable structure, low detection cost and good stability

Inactive Publication Date: 2014-12-24
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have their own shortcomings. The process of virus isolation and identification and neutralization test is cumbersome and time-consuming; electron microscope technology is difficult to apply in the basics; molecular biology methods have high requirements for technology and equipment, and these detection methods are difficult to be used in veterinary clinic application

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  • Immune colloidal gold test stripe and preparation method and application

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Effect test

Embodiment 1

[0031] The present invention will be further described below in conjunction with drawings and embodiments.

[0032] An immune colloidal gold test strip for detecting canine adenovirus type Ⅱ, which consists of a rigid polyvinyl chloride backing plate 1, a nitrocellulose membrane 2, a colloidal gold binding pad 4, a sample pad 3, a water-absorbing pad 5, and a detection line 6 , composed of quality control line 7, characterized in that: nitrocellulose membrane 2 is pasted on the rigid polyvinyl chloride backing plate 1, and colloidal gold bonding pads 4, The sample pad 3 and the absorbent pad 5 are arranged on the right side above the nitrocellulose membrane 2 . The colloidal gold binding pad 4 is made of glass fiber. The colloidal gold binding pad 4 is soaked in buffer solution for 30 minutes, dried at 37° C., and sprayed with colloidal gold-labeled anti-canine adenovirus type II monoclonal antibody. The nitrocellulose membrane 2 is the detection membrane of the test strip, t...

Embodiment 2

[0034] A preparation method of an immune colloidal gold test strip for detecting canine adenovirus type Ⅱ, the steps of which are:

[0035] (1) Prepare colloidal gold by reacting trisodium citrate with chloroauric acid: put 100 mL of water for injection into the silicified bottle, then add 1.2 mL of 1% (w / v) chloroauric acid, and heat it on an electric furnace (electric furnace Adjust the power to 1500W, 200-300°C) to complete boiling; quickly add 1.8 mL of 1.05% (w / v) trisodium citrate aqueous solution at one time, and continue heating (adjust the power of the electric furnace to 1500W, 200-300°C) until the solution becomes Turn into wine red, cool down at room temperature (20-25°C) naturally to form a colloidal gold solution. The appearance of the prepared colloidal gold is clear and transparent wine red, the spectrum is scanned at 450-600 nm by a spectrophotometer, and the maximum absorption wavelength is 520 nm, and the particle size is uniform when observed by an electro...

Embodiment 3

[0048] An application of an immune colloidal gold test strip in detecting canine adenovirus type Ⅱ, the steps are:

[0049] (1) Pretreatment of samples: Collected samples such as dog nasal mucosa swabs, feces or organ samples of dead dogs, each with a negative sample as a control. Take about 0.5 g (mL) of each sample and add about 0.5 mL of normal saline to make a suspension (mix the stool sample thoroughly and let it stand for 5 min, or centrifuge).

[0050] (2) Detection: Take 2-4 drops (70-140 μL) of the supernatant and drop them on the test paper of the present invention, and observe the results after 15 minutes.

[0051] (3) Judgment of results: When red appears on both the quality control line and the detection line, it is positive for canine adenovirus type Ⅱ, that is, the sample contains canine adenovirus type Ⅱ, and if the detection line does not appear red, it is negative for canine adenovirus type Ⅱ. That is, the sample does not contain canine adenovirus type Ⅱ. I...

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Abstract

The invention discloses an immune colloidal gold test stripe and preparation method and application, and the immune colloidal gold test stripe comprises a rigid polyvinyl chloride backer board, a nitrocellulose film, a sample mat, a colloidal gold combination mat and a water absorption mat, the nitrocellulose film is pasted to the rigid polyvinyl chloride backer board, the colloidal gold combination mat is pasted to one end of the nitrocellulose film, the sample mat is pasted on the colloidal gold combination mat, the water absorption mat is located at the other end of the nitrocellulose film; the preparation method comprises the steps as follows: (1) having reaction on the trisodium citrate and chloroauric acid for preparing colloidal gold; (2) marking the canine adenovirus resisting virus II type monoclonal antibody on the colloidal gold test stripe; (3) covering the canine adenovirus resisting virus II type antibody on the nitrocellulose film; (4) orderly pasting together for obtaining colloidal gold test stripe. The application of the immune colloidal gold test stripe for detecting canine adenovirus virus II type, the test stripe is simple in operation, fast and sensitive in detection, clear in result and easy in judgment. The immune colloidal gold test stripe is convenient to carry and use for saving the sickness detection cost.

Description

technical field [0001] The invention relates to the technical field of detection of canine epidemics, in particular to an immune colloidal gold test strip, and also relates to a preparation method of an immune colloidal gold test strip, and also relates to an immune colloidal gold test strip in the detection of Use in canine adenovirus type II. Background technique [0002] Canine adenovirus type Ⅱ (CAV-Ⅱ) can cause recessive or mild infection of the respiratory tract in dogs, and may cause infectious laryngotracheitis and pneumonia symptoms in dogs. Clinical features include persistent high fever, cough, serous to mucous rhinorrhea, tonsillitis, laryngotracheitis, and pneumonia. It often occurs in puppies under half a year old. In unweaned or newly weaned puppies can cause coughing in the whole litter or group. CAV-II can be infected through the mouth and nose. The virus replicates in ciliated bronchiole epithelial cells, nasal mucosa, surface cells of the pharynx and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N33/532
Inventor 徐高原贾慧勤董晓辉但汉并
Owner WUHAN KEQIAN BIOLOGY CO LTD
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