The invention discloses a regulation method of production of
taxane by long-period culture of
taxus chinensis cells. The regulation method comprises the following steps: after carrying out
suspension culture on
taxus chinensis
cell liquid for 50 generations, adopting a
silver nitrate water solution added with a wide weak regulation agent at a first phase; sub-culturing by adopting a weak regulation manner with the concentration of 1mM to 40mM; at a second phase, producing
paclitaxel and the
taxane through a moderate intensity regulation manner by adopting a strong regulation agent, wherein at the second phase, the strong regulation agent is a
coronatine water solution, the utilization concentration is 10nM to 2000nM, the number of days for adding
coronatine is 1 day to 8 days; or the strong regulation agent is a
jasmonic acid type
ethanol solution, the utilization concentration is 10nM to 200nM, the number of days for adding
jasmonic acid is 1 day to 8 days. At a subculture phase, the weak regulation agent also is added; at a production phase, a moderate intensity regulation agent is added; in a long-period culture process, the culture of
plant cells reaches harmonious join of the two phases; finally, high-yield and stable-yield
paclitaxel and the
taxane midbody 10DBA and BAT III are obtained.