51 results about "Coronatine" patented technology

The invention discloses a drought-resistant agent and application thereof. The plant drought-resistant agent comprises the active ingredients such as coronatine (COR) and S-abscisic acid (S-ABA). Compared with the present substance for improving the drought resistance of crops, the drought-resistant agent has the following characteristics: unexpected synergy can be obtained through COR, S-ABA and other plant growth adjusting substances which are added on the basis of the COR and S-ABA; an expected addition effect on the growth of crops and the adjustment of the activities can be exceeded, and on one hand, the crop application range can be enlarged, and on the other hand, the application concentration of coronatine, S-ABA and other plant growth adjusting agents can be reduced; and moreover, under the condition that the concentration of a single compound is lower than the effective concentration, a good adjusting degree on the growth of plants can be obtained through the plant growth adjusting agent.

The invention discloses a method for preparing coronatine and a special bacterium thereof. The bacterium provided by the invention is a pseudomonas syringae pv.glycinea with a preservation number of CGMCC No.2533. The bacterium MW123 (with the preservation number of CGMCC No.2533) is an excellent bacterium of a coronatine with high output. Test result shows that the method for using the bacterium MW123 (with the preservation number of CGMCC No.2533) to prepare the coronatine has the advantage of high and stable output; the output can achieve 180mg / L to 200mg / L offermentation broth. Compared with the original bateriusm, the output is improved by 3 to 4 times. Therefore, the method of the invention for preparing the coronatine solves the difficulty of low output of the coronatine of the existing fermentation production and is applicable for industrial popularization and application.

The invention relates to a rooting agent formula for taxus chinensis branch cutting rooting. The rooting agent is prepared from, by mass, 200 mg-300 mg of indolebutyric acid, 200 mg-300 mg of naphthylacetic acid, 20 mg-30 mg of jasmonic acid methyl ester, 0.1 mg-0.5 mg of coronatine, 10 mg-20 mg of phenylalanine, 10 mg-20 mg of salicylic acid, 50 mg-100 mg of vitamin B2, 5 mg-10 mg of vitamin B6, 50 mg-100 mg of vitamin B12, 1 mg-5 mg of vitamin C, 1 mg-5 mg of vitamin H and 2 g-5 g of saccharose. According to the rooting agent formula for taxus chinensis branch cutting rooting, the content of chlorophyll in cutting branches and activity of enzymes in the branches can be improved, and growth of root systems and the survival rate of the cutting branches are effectively promoted. The invention further provides a preparation method of the rooting agent formula for taxus chinensis branch cutting rooting.

The present invention relates to gene engineering bacterial strain, and is especially one kind of soybean disease causing variety (P.syringae pv.Glycinea) of pseudomonas syringae for producing crown bacterin and its construction process. The gene engineering pseudomonas strain is prepared through mutagenizing initial pseudomonas strain with nitrosoguanidine for genetic mutation, so as to screen out pseudomonas strain with high crown bacterin yield. Experiment proves that the gene engineering pseudomonas strain of the present invention may be used in industrial production of crown bacterin through fermentation at 18deg.c to reach the yield of 84.7-112 mg / L.

The invention relates to a formula of a rooting agent, in particular to a rooting agent applicable to cutting seedling raising of green branches of camellia nitidissima. The rooting agent applicable to cutting seedling raising of the green branches of the camellia nitidissima is characterized in that the rooting agent is prepared from raw materials in parts by weight as follows: 3-10 parts of naphthalene acetamide, 10-15 parts of 3-indolebutyric acid, 10-15 parts of naphthylacetic acid, 3-10 parts of a pinellia ternate extracting solution, 3-10 parts of a chrysanthemum extracting solution, 5-8 parts of glycine, 5-8 parts of glutamic acid, 3-8 parts of methyl jasmonate, 3-10 parts of coronatine, 3-12 parts of vitamin B2, 3-12 parts of vitamin B6, 3-12 parts of vitamin B12, 3-7 parts of vitamin C and 2-7 parts of vitamin H. The rooting effect of the camellia nitidissima is effectively improved, the pest and disease damage resistance is improved, and root nutrition of the camellia nitidissima is effectively supplemented.

The invention relates to a simple pretreatment method of coronatine biological detection method. The technical scheme of the invention is the application of the coronatine in restraining seeds bourgeoning. Among the invention, the normal pretreatment for seeds is that: spreading a layer of filter paper capable of keeping moisture at bottom of a clean cultivating vessel and placing the seeds to be pretreated on the filter paper evenly and dispersedly with 10 grains to 100 grains for each cultivating vessel; getting the upper supernatant liquid after removing the impurity which affecting the detection through decentralization in the coronatine sample solution detected and injecting 10 mL to 40 mL upper supernatant liquid into the cultivating vessel containing the seeds; then after one to seven days dark cultivation with the temperature of 20 DEG C.to 35 DEG C., measuring the length of the seeds roots after bourgeoning. Compared with the high efficiency liquid chromatography and the ELISA, the method has the advantages of convenient operation, economy and easy for promotion and publicity as well as high detection sensitivity. Besides, the invention provides the new purpose of the coronatine.

The present invention discloses a method for extracting coronatine, in which coronatine is generated through eluting and separating supernatant of microorganism fermentation liquids containing coronatine with adsorption column that takes macroporous adsorption resin as adsorbing agent. The method of present invention has the features of high extraction yield for coronatine, simple operation, low production cost and low environment pollution.

The present application relates to a nano particle compositions and methods for targeted delivery of a bioactive agent to a plant. In one embodiment, the nano particle composition includes a coronatine-coated nano particle formulated to deliver one or more bioactive agent through plant stomata. A variety of bioactive agents may be included in the nano particles, including one or more bactericides,fungicides, insecticides, acaricides, miticides, nemanticides, molluscicides, herbicides, plant nutrients, fertilizers, plant growth regulators, or combinations thereof.

The invention relates to a glycyrrhiza glabra callus cell culture method being capable of improving content of licoflavone. The method is characterized by including the steps of: (1) choosing full glycyrrhiza glabra seeds, and culturing the seeds on a MS basic culture medium containing 30 g / L of saccharose and 7 g / L of agar to obtain sterile seedlings, and inoculating the sterile seedlings on a callus induction culture medium for cultivation, and moving the sterile seedlings on a subculture medium for subculture; (2) selecting slight-yellow callus after the subculture in the step (1), and transferring the callus on a liquid culture medium to perform suspended shaking cultivation; (3) performing inductive cultivation during the suspended shaking cultivation process of the callus, and increasing culturing temperature from 25 DEG C to 35-45 DEG C from the sixth to twelfth day of the subculture period, and continuously culturing the callus for 1-3 days, reducing the culturing temperature to 25 DEG C, wherein coronatine is added during the cultivation at 35-45 DEG C and p-hydroxyphenylpyruvic acid is added when the temperature is reduced to 25 DEG C, and after the suspended shaking cultivation is finished, the glycyrrhiza glabra callus cell having high content of licoflavone can be obtained. The method achieves high content of the licoflavone.

The invention provides a method for discovering a drug action mechanism by transcriptomics for co-expressing cyclic circadian rhythm. The method comprises the steps: successfully identifying a molecular mechanism of a drug action by analyzing deep sequencing circadian time sequence transcriptome data in combination with bioinformatics, coexpression analysis, machine learning and data visualizationtechnologies; and establishing an mRNA expression network by utilizing mRNA expression data, dividing modules, carrying out functional enrichment analysis on GO and KEGG of each module by utilizing hypergeometric distribution, extracting the module overcoming the cyclic rhythm of the module as a main explanation for an endogenous molecular mechanism of biological phenotype change related to corresponding drug treatment, and comprehensively explaining and assessing the molecular mechanism of a drug for a physiological function of a corresponding biological representation by utilizing data visualization in combination with phenotypic change and functional analysis after drug treatment. The invention further provides an application of the method to the research of an action mechanism of coronatine inhibiting growth and promoting defense and the discovery of action mechanisms and toxic / side effect mechanisms of pesticides, medicines, veterinary drugs and drugs for aquatic products.

Stable genetically engineered bacterial strains that overproduce coronatine are provided. The stable strains can be successfully cultivated to overproduce coronatine at temperatures that are suitable for large scale, commercial preparations of coronatine. The overproducing strains are also non-pathogenic. An exemplary strain is Pseudomonas syringae APV1, which successfully overproduces coronatine at 26° C. Methods of optimizing culture conditions for coronatine production from the novel stable overproducing strains are provided, as are methods for using the overproducing strains to induce abscission and increase taxane production.

The invention discloses a Pseudomonas syringae pv.mori bacterial strain for producing coronatine and a method for producing coronatine by the fermentation thereof. The Pseudomonas syringae pv.mori bacterial strain M4-13 is preserved on 1st, Feb 2010 with preservation number of CGMCC No.3621. The Pseudomonas syringae pv.mori bacterial strain M4-13 generates coronatine activity at a high temperature of 32 DEG C.

The invention discloses a method for extracting coronatine from fermentation liquor by using a membrane separation technique, comprising micro-filtration and hyper-filtration steps: filtering a fermentation liquor containing coronatine by a micro filtering membrane with the aperture of 0.1-10 Mum so as to remove impurity particles and thalli; then filtering the liquor by a hyper-filtration membrane with the aperture of 0.001-0.1 Mum so as to remove macromolecule impurities of proteins, nucleic acids and colloid granules to obtain clear filtrate; and then carrying out common resin exchange or column chromatography, concentrating and crystallizing to obtain the coronatine. In order to improve the effect of separating purification, pre-treatment can be added before the micro-filtration, namely, the coronatine fermentation liquor is filtered though the filtration membrane with the aperture of 1-10 Mum; nano-filtration can be added after the hyper-filtration, namely, the effluence liquor isfiltered by a nano-membrane system with cut-off molecular weight of 400-800. The method in the invention has simple operation and high yield which can be over 90%; in addition, the method has no influence on the structure and activity of the coronatine; and has low energy consumption and low pollution, thus completely satisfying the requirements of industrialized production.

The present invention relates to a method of producing a taxane-type diterpene wherein a cell and / or a tissue of a plant which produces the taxane-type diterpene is cultured in the presence of at least one substance selected from the group consisting of coronatines, a bacterium which produces the coronatines, a culture solution or a culture extract of such bacterium, cyclic polysaccharides, fatty acids, and an imino or amino derivative of jasmonic acids, then the taxane-type diterpene is recovered from the resulting cultures.

The invention discloses an efficient cotton defoliating agent and a synthesis method thereof. The defoliating agent is prepared from, by weight, 20-40 parts of ethephon, 10-20 parts of magnesium chlorate, 10-25 parts of thidiazuron, 12-22 parts of coronatine, 8-15 parts of polycondensated naphthalenesulfonate, 2-6 parts of carrageenan, 10-20 parts of moist organosilicon auxiliary, 15-20 parts of sodium lignin sulfonate, 8-17 parts of hydroxyquinoline, 3-10 parts of ethylene glycol and 35-55 parts of deionized water. The preparation process is simple, the prepared defoliating agent can improve the cotton defoliating rate, is not harmful to cotton and has environment-friendly and non-toxic effect. In addition, the residues of the efficient cotton defoliating agent do not cause soil pollution.

The invention relates to application of coronatine in crop vigorous growth control, and belongs to the technical field of crop cultivation. The technical problem to be solved by the invention is to provide a new application of the coronatine, namely, the application of the coronatine in crop vigorous growth control, and the application concentration of the coronatine is greater than 1ppm. The invention provides the new application of the coronatine as a crop vigorous growth control agent, the coronatine can be diluted and then sprayed on crop leaves, and the purpose of controlling vigorous growth of crops is achieved. The agent is low in use concentration and low in cost, the coronatine belongs to a natural product and is good in environmental compatibility, and the problem of agent residues does not exist.

The invention discloses a coronatine aqueous solution and a preparation method thereof. The coronatine aqueous solution having functions of a plant-growth regulator and comprises coronatine, a surfactant, an antifreezing agent, an antifoaming agent, an antiseptic and water. The coronatine aqueous solution also comprises an organic solvent. 100 mass parts of the coronatine aqueous solution comprises 0.0001-0.1 mass parts of coronatine, 0.5-5 mass parts of the surfactant, 2-10 mass parts of the antifreezing agent, 0.1-1 mass part of the antifoaming agent, 0.1-1 mass part of the antiseptic, 0.1-5 mass parts of an organic solvent and the balance water. The coronatine aqueous solution has reasonable coronatine content, adopts a small amount of the organic solvent, does not product dust drifting, does not burn and explode easily, has high biological activity, is safe and environmentally friendly, effectively improves a plant object yield and improves quality.

Stable genetically engineered bacterial strains that overproduce coronatine are provided. The stable strains can be successfully cultivated to overproduce coronatine at temperatures that are suitable for large scale, commercial preparations of coronatine. The overproducing strains are also non-pathogenic. An exemplary strain is Pseudomonas syringae APV1, which successfully overproduces coronatine at 26° C. Methods of optimizing culture conditions for coronatine production from the novel stable overproducing strains are provided, as are methods for using the overproducing strains to induce abscission and increase taxane production.

The invention discloses a cotton defoliation compound and a preparation method thereof. The defoliation compound contains the following components in parts by weight: 15-30 parts of ethephon, 10-20 parts of naphthylacetic acid, 8-15 parts of simethicone, 5-10 parts of coronatine, 8-20 parts of abscisic acid, 2-6 parts of glycol, 10-20 parts of sodium lignin sulfonate, 8-15 parts of poly-condensed naphthalene sulfonate, 5-10 parts of nonyl phenol polyoxyethylene ether, 5-10 parts of zinc sulfate, 1-4 parts of nanometer silver ion powder and 20-30 parts of deionized water. According to the invention, the technology is simple; the prepared defoliation agent can increase the defoliation rate of the cotton, is harmless to cotton, is capable of effectively reducing impurity content and is capable of increasing the cotton fiber quality; besides, the residue thereof cannot pollute the soil.

The invention discloses a method for extracting coronatine from fermentation liquor by using a membrane separation technique, comprising micro-filtration and hyper-filtration steps: filtering a fermentation liquor containing coronatine by a micro filtering membrane with the aperture of 0.1-10 Mum so as to remove impurity particles and thalli; then filtering the liquor by a hyper-filtration membrane with the aperture of 0.001-0.1 Mum so as to remove macromolecule impurities of proteins, nucleic acids and colloid granules to obtain clear filtrate; and then carrying out common resin exchange or column chromatography, concentrating and crystallizing to obtain the coronatine. In order to improve the effect of separating purification, pre-treatment can be added before the micro-filtration, namely, the coronatine fermentation liquor is filtered though the filtration membrane with the aperture of 1-10 Mum; nano-filtration can be added after the hyper-filtration, namely, the effluence liquor is filtered by a nano-membrane system with cut-off molecular weight of 400-800. The method in the invention has simple operation and high yield which can be over 90%; in addition, the method has no influence on the structure and activity of the coronatine; and has low energy consumption and low pollution, thus completely satisfying the requirements of industrialized production.

The invention provides a regulation and control method for culturing saussurea involucrata cells in a scale manner for high-producing flavone. According to the technical scheme, first, an initial cell strain is quickly cultured in a liquid suspension way, passage is carried out once for every 7 to 10 days, and the increments of the fresh weights of cells are 3 to 5 times; subsequently, a two-stage culture method is adopted, the cells are enabled to quickly proliferate at a growth stage, the maximum cell concentration is obtained within 7 to 10 days, a precursor phenylalanine and an inducer coronatine are added into the cells in the condition of a certain high-density inoculum concentration of 150g / l to 400g / l (a fresh weight) at a production stage, the cells are harvested after being cultured for 3 to 5 days, and the flavone content is increased to 13 percent or above. The novel inducer coronatine is adopted for the process; the use level is few; the effect is obvious; no toxic or side effects exist; the flavone content of the saussurea involucrata cells can be quickly improved; the regulation and control method is applicable to a large-scale bioreactor of 20L or larger; the cells are already successfully cultured on scales of 20L, 100L, 200L and 1,000L by utilizing a bioreactor from the company of the inventor, furthermore, the cellular state is good, the yield is stable and the flavone content is 11 percent or above.

The invention discloses a cotton defoliating agent. The cotton defoliating agent is prepared by mixing the following components in parts by weight: 40-50 parts of magnesium chlorate, 6-8 parts of paclobutrazol, 20-40 parts of ethephon, 3-5 parts of thidiazuron, 2-4 parts of coronatine and 10-20 parts of a pesticide synergist. The product disclosed by the invention has the characteristics of small sizes, large active surface, strong permeability, no dust in a pesticide preparation process, low cost, high pesticide effect, cold prevention and frost resistance and the like.

The invention relates to the technical field of application of plant growth regulators, and particularly discloses a new branch tip vigorous growth control agent for green prickleyash and a new branch tip vigorous growth control method of the new branch tip vigorous growth control agent. The vigorous growth control agent for the new shoots contains prohexadione calcium and a trace amount of S-abscisic acid and / or coronatine as effective components, and contains an agriculturally acceptable auxiliary agent, the mass of the prohexadione calcium accounts for 5%-15% of the mass of the vigorous growth control agent for the new shoots, and the weight ratio of the prohexadione calcium to the S-abscisic acid to the coronatine is 1000: (1-5): (0.1-0.5). The vigorous growth control agent for the new branch tips is dissolved and diluted to 500-1500 times, the vigorous growth control agent is used when the new branch tips of the zanthoxylum bungeanum are 30-50 cm, then the vigorous growth control agent is used once every 15-25 cm of the new branch tips, the vigorous growth control agent is used 2-4 times in total, and the vigorous growth control agent has remarkable effects on vigorous growth control of the new branch tips of the zanthoxylum schinifolium, promotion of flowering and fruit setting of the zanthoxylum schinifolium and improvement of the unit yield of

The invention discloses a regulation method of production of taxane by long-period culture of taxus chinensis cells. The regulation method comprises the following steps: after carrying out suspension culture on taxus chinensis cell liquid for 50 generations, adopting a silver nitrate water solution added with a wide weak regulation agent at a first phase; sub-culturing by adopting a weak regulation manner with the concentration of 1mM to 40mM; at a second phase, producing paclitaxel and the taxane through a moderate intensity regulation manner by adopting a strong regulation agent, wherein at the second phase, the strong regulation agent is a coronatine water solution, the utilization concentration is 10nM to 2000nM, the number of days for adding coronatine is 1 day to 8 days; or the strong regulation agent is a jasmonic acid type ethanol solution, the utilization concentration is 10nM to 200nM, the number of days for adding jasmonic acid is 1 day to 8 days. At a subculture phase, the weak regulation agent also is added; at a production phase, a moderate intensity regulation agent is added; in a long-period culture process, the culture of plant cells reaches harmonious join of the two phases; finally, high-yield and stable-yield paclitaxel and the taxane midbody 10DBA and BAT III are obtained.

The invention discloses a plant growth regulator composition containing brassinolide, chlorine iron and coronatine and application thereof. The plant growth regulator composition is prepared from the following components in parts by weight: 0.001 to 1 part of brassinolide, 0.001 to 0.1 part of chlorine iron and 0.01 to 10 parts of coronatine. According to the invention, the activation performance of various enzymes can be improved, the metabolism of plants is accelerated, and the growth of crops is promoted; crops are induced to improve the resistance to cold injury and over-low-temperature cold injury period safely; the composition has significant effects in the aspects of synergism of nutrient balance in plants, increase of yield and improvement of quality.

The invention relates to a genetically engineered bacterium (Pseudomonas syringae soybean pathogenic variety CGMCC 1276), its construction method and its use. The engineering bacterium is obtained by inserting the Tn5 transposon S17-1 into the genome of the starting bacterium to inactivate its temperature control gene. The strain can be used for industrial fermentation to produce coronatine at the fermentation temperature of 26°C-28°C. The yield is similar to that of the starting bacteria (fermentation temperature is 18°C), and can reach 35mg / l-40mg / l.

The invention discloses a cotton defoliating agent. The cotton defoliating agent is prepared by mixing, by weight, 6-10 parts of paclobutrazol, 6-8 parts of ethephon, 1-3 parts of silky organic silicone surfactant, 15-25 parts of chlorate, 6-8 parts of coronatine, 6-10 parts of xanthan gum and 6-8 parts of thidiazuron. The cotton defoliating agent is harmless to plants, and boll opening is normal.

The invention relates to the technical field of wheat cultivation, in particular to a compound agent for improving cold resistance of wheat in spring, an application method and application of the compound agent. Within 24 h of low-temperature stress pretreatment, a compound mixed solution (containing Tween-20 with the final concentration of 3 vol%) of 20 mg.L<-1> of 16-BA, 100 mg.L<-1> of chitosan oligosaccharide, 0.1 mg.L<-1> of 124-epibrassinolide and 0.01 micromol.L<-1> of coronatine is externally sprayed. The photosynthetic performance of flag leaves can be remarkably improved, normal development of florets is guaranteed, the influence of low temperature on the growth process of wheat is relieved, wheat yield loss caused by low-temperature stress is relieved, and the effect of relieving low-temperature stress of wheat in the booting stage is remarkable.

The invention discloses a synthesis method for a cotton defoliant. The defoliant is prepared from, by weight, 20-40 parts of ethephon, 20-50 parts of magnesium chlorate, 15-25 parts of paclobutrazol, 10-15 parts of coronatine, 10-20 parts of pyraflufen-ethyl, 2-6 parts of carrageenan, 15-20 parts of sodium lignosulphonate, 5-12 parts of sodium carboxymethylcellulose, 2-6 parts of nano-silver ion powder and 25-45 parts of deionized water. The preparation process is simple, the obtained defoliant can increase the cotton defoliation rate, is harmless to cotton and has the advantages of being environmentally friendly and free of toxicity, and residues of the defoliant will not contaminate soil.