Application of coronary toxin in restraining seed germination and the simple preprocessing method of coronary toxin biological detecting method thereof

Abstract

The invention relates to a simple pretreatment method of coronatine biological detection method. The technical scheme of the invention is the application of the coronatine in restraining seeds bourgeoning. Among the invention, the normal pretreatment for seeds is that: spreading a layer of filter paper capable of keeping moisture at bottom of a clean cultivating vessel and placing the seeds to be pretreated on the filter paper evenly and dispersedly with 10 grains to 100 grains for each cultivating vessel; getting the upper supernatant liquid after removing the impurity which affecting the detection through decentralization in the coronatine sample solution detected and injecting 10 mL to 40 mL upper supernatant liquid into the cultivating vessel containing the seeds; then after one to seven days dark cultivation with the temperature of 20 DEG C.to 35 DEG C., measuring the length of the seeds roots after bourgeoning. Compared with the high efficiency liquid chromatography and the ELISA, the method has the advantages of convenient operation, economy and easy for promotion and publicity as well as high detection sensitivity. Besides, the invention provides the new purpose of the coronatine.

Description

technical field [0001] The invention relates to the application of corona toxin in inhibiting seed germination and a simple pretreatment method of a corona toxin biological detection method. Background technique [0002] The sown areas of rice and wheat in my country each account for more than 20 million hectares, with a total output of more than 100 million tons. They have a fairly long storage period from harvest to sowing the following year, usually 3-8 months or longer. How to avoid the decline of seed viability during the storage period is the focus of attention of various sub-business units. However, the air humidity in the rice production area is relatively high, and the moisture in the seed storage is too high, or it can be dampened or rained after storage, which can cause it to germinate. In addition, if rice, wheat and corn seeds encounter hot and humid weather before harvesting, they often germinate on the ear—ear germination. Ear germination is a problem often ...

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography has high requirements for pretreatment, and the cost of use and maintenance is high; the sensitivity of ELISA technology reaches the nanogram level, but the antibody used for its measurement is not commercialized, and the cost of use is very high

Therefore the above-mentioned corona toxin detection method is not practical in industrial production