Pseudomonas syringae pv.mori bacterial strain for producing coronatine and method for producing coronatine by fermentation thereof

A technology of pseudomonas and coronatine, applied in the field of microorganisms, can solve the problems of high cost of cooling equipment and so on

Inactive Publication Date: 2011-01-19
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the production of COR by large-scale fermentation will inevitably generate a large amount of heat, and the cost of cooling equipment is expensive, and the use of chemical synthesis cannot meet the needs of large-scale applications. Basis for large-scale production of coronatins

Method used

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  • Pseudomonas syringae pv.mori bacterial strain for producing coronatine and method for producing coronatine by fermentation thereof
  • Pseudomonas syringae pv.mori bacterial strain for producing coronatine and method for producing coronatine by fermentation thereof
  • Pseudomonas syringae pv.mori bacterial strain for producing coronatine and method for producing coronatine by fermentation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This example illustrates the screening process of Pseudomonas syringae pv.mori M4-13 strain.

[0016] Collect mulberry branches and leaves infected with mulberry blight, mash them into a conical flask with 50mL of sterile water, add about 20 glass beads, shake vigorously for 20 minutes to completely mash the tissues, and let stand. Add 1mL of soil sample suspension into each 50mL sterile water Erlenmeyer flask, put it into a constant temperature shaking incubator, and incubate at 37°C for 2 days.

[0017] Take the suspension and spread it on the primary screening MG plate medium to carry out the primary screening of the enzyme-producing strains. The components of each liter of primary screening medium are: peptone 5.0g, mannitol 5.0g, sodium glutamate 1.15g, biotin 0.0001g, dipotassium hydrogen phosphate 0.25g, sodium chloride 0.1g, magnesium sulfate heptahydrate 0.1 g, agar 11g. The temperature is 32°C, and the cultivation time is 1 to 6 days. Select 20 strains that...

Embodiment 2

[0021] This example illustrates the coronatin activity of Pseudomonas syringae pv.mori M4-13 strain based on standard LB culture at 18°C.

[0022] Per liter medium composition: peptone 10g, yeast extract 5g, sodium chloride 10g, Pseudomonas syringae pv.mori (Pseudomonas syringae pv. After aerobic culture at 18°C ​​for 7 days, the fermentation broth was tested by HPLC, and the concentration was 0.54mg / L.

Embodiment 3

[0024] The method of this example is the same as that of Example 2, and the optimized HSC medium is used instead for culture, and its coratin-producing activity is detected.

[0025]The composition of each liter of medium: 4.1 g of dipotassium hydrogen phosphate, 3.6 g of potassium dihydrogen phosphate, 1 g of ammonium chloride, 0.2 g of magnesium sulfate heptahydrate, 2 μM of ferric chloride, and 20 g of glucose, Pseudomonas syringaepv .mori) M4-13 was inoculated in the culture medium with an inoculum size of 1%, and cultured aerobically at a temperature of 18° C. for 7 days, and the fermentation broth was tested by HPLC, and the concentration was 2 mg / L.

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Abstract

The invention discloses a Pseudomonas syringae pv.mori bacterial strain for producing coronatine and a method for producing coronatine by the fermentation thereof. The Pseudomonas syringae pv.mori bacterial strain M4-13 is preserved on 1st, Feb 2010 with preservation number of CGMCC No.3621. The Pseudomonas syringae pv.mori bacterial strain M4-13 generates coronatine activity at a high temperature of 32 DEG C.

Description

1. Technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a coronatin-producing Pseudomonas mori syringae strain M4-13 and a method for fermenting and producing coronatin. 2. Background technology [0002] Prior art: coronatine (Coronatine, COR, C 18 h 25 NO 4 ) was first isolated from the culture medium of Pseudomonas syingaepv.atropurpurea by Ichihara et al. in 1977. Coronatine is composed of two special parts, namely: bicyclic carboxylic acid, called Coronafacic acid (CFA for short) and a cyclopropyl amino acid, called Coronamic acid (CMA for short). [0003] As a new multifunctional compound plant hormone, coronatin has a wide range of functions and application prospects. It is not only related to growth and development, but also has a significant relationship with the plant's own defense system. It can regulate growth, inhibit aging, promote cell differentiation, increase chlorophyll content and plant stress re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/02C12R1/38
Inventor 吴福安王俊梁垚陈明胜方水琴吕荣斌
Owner JIANGSU UNIV OF SCI & TECH
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