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Method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate

A technology of methyl jasmonate and Haematococcus pluvialis, applied in the biological field, can solve the problems of complex technical process, high equipment requirements, high cost, etc., and achieve the effect of improving accumulation efficiency and low cost

Inactive Publication Date: 2011-02-16
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned relevant patented technologies are advanced, they all involve professional facilities such as photobioreactors, which require relatively high equipment, complicated technical processes, difficult operations, and high costs, which undoubtedly increase the production cost and the difficulty of technology promotion.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Step 1. Preparation of algae liquid: Haematococcus pluvialis was selected from 712 algae strains, using MCM medium, cultured at a temperature of 19°C, a light intensity of 1500lx, and a light-to-dark ratio of 12h / 12h, and cultivated under fluorescent lamps until pluvialis red The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0016] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: the methyl jasmonate produced by SIGMA was used. Add the methyl jasmonate stock solution with a mass percentage concentration of 95% to the above-mentioned cultured algae liquid until the methyl jasmonate concentration is 4.95 mg / L; then add the above-mentioned algal liquid with methyl jasmonate and the blank The algae liquid of the control group was cultured under the combined stress conditions of 25°C, light intensity 4500 lx, continuous sunlight and nutri...

Embodiment 2

[0018] Step 1. Preparation of algae liquid: Haematococcus pluvialis is selected from 712 algae strains, using MCM medium, cultivated under the conditions of temperature 20°C, light intensity 1800lx, and light-to-dark ratio 12h / 12h, and cultivated under fluorescent lamp until Pluvialls red The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0019] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: the methyl jasmonate produced by SIGMA was used. Add the methyl jasmonate stock solution with a mass percentage concentration of 95% to the above-mentioned cultured algae liquid until the concentration of methyl jasmonate is 5.0mg / L; then add the above-mentioned algae liquid with methyl jasmonate and the blank The algae liquid of the control group was cultured under the combined stress conditions of 26°C, light intensity of 4800lx, continuous sunlight ...

Embodiment 3

[0021] Step 1. Preparation of algae liquid: Haematococcus pluvialis is selected from 712 algae strains, using MCM medium, cultured at a temperature of 21°C, a light intensity of 1600lx, and a light-to-dark ratio of 12h / 12h, and cultivated under a fluorescent lamp until pluvialis The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0022] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: the methyl jasmonate produced by SIGMA was used. Add the methyl jasmonate stock solution with a mass percentage concentration of 95% to the above-mentioned cultured algae liquid until the concentration of methyl jasmonate is 5.05mg / L; then add the above-mentioned algae liquid with methyl jasmonate and the blank The algae liquid of the control group was cultured under the combined stress conditions of 28°C, light intensity of 5000 lx, continuous sunlight and nutr...

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Abstract

The invention provides a method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate, which comprises the following steps: (1) culturing methyl jasmonate cells to exponential phase so as to obtain cultured microalgal liquid for induction; and (2) accumulation of astaxanthin: adding methyl jasmonate concentrate the mass concentration of which is 95 percent into cultured microalgal liquid until the final concentration of the methyl jasmonate is 4.95 to 5.05 mg / L; then culturing the microalgal liquid added with methyl jasmonate until the haematococcus pluvialis cells are completely red under an optical microscope at a temperature of 25 to 28 DEG C and an optical intensity of 4500 to 5000lx and under the conditions of continuous illumination of fluorescent lamps and nutritive salt starved compound stresses so as to finish the accumulation of astaxanthin, wherein at the moment of finishing the accumulation of astaxanthin, the maximum output of the astaxanthin is reached. The invention is simple, easy to operate and low in cost, can greatly shorten the time that the haematococcus pluvialis cells are completely red, and significantly increase the output of the astaxanthin, thereby greatly improving the efficiency of producing astaxanthin by haematococcus pluvialis.

Description

technical field [0001] The invention provides a method for using methyl jasmonate to induce Haematococcus pluvialls to produce astaxanthin, and belongs to the field of biotechnology. Background technique [0002] Haematococcus pluvialis is recognized as the organism with the highest natural astaxanthin content in nature at present, and its accumulation can be as high as 4.0% of the dry cell weight, which is 1000-4000 times that of salmon meat and far higher Astaxanthin (0.15%-0.4%) produced by fermentation of Phaffia rhodoxyma. Astaxanthin molecules not only have long conjugated double bonds like other carotenoids, but also have unsaturated ketone and hydroxyl groups at the end of the conjugated double bonds, and the hydroxyl and ketone groups form α-hydroxy ketones. This structure endows astaxanthin with more prominent physiological functions than general carotenoids, which are mainly reflected in the following two aspects: First, astaxanthin, like other carotenoids, has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/89
Inventor 高政权孟春晓叶乃好高瑞照罗韬王依涛
Owner SHANDONG UNIV OF TECH
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