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Identification and application of cotton gene BZR1

A cotton and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem that the mechanism of action affecting the development of functional cotton fibers is not clear.

Inactive Publication Date: 2014-08-13
HUAZHONG NORMAL UNIV
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Problems solved by technology

Although some genes related to hormone signaling have been isolated and identified in cotton, the function of these genes in the cotton hormone signal transmission pathway and the mechanism of action on cotton fiber development are still unclear, and some key regulatory proteins have yet to be cloned Identify and elucidate their function

Method used

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  • Identification and application of cotton gene BZR1
  • Identification and application of cotton gene BZR1
  • Identification and application of cotton gene BZR1

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Embodiment Construction

[0024] The present invention is further illustrated by the following drawings and implementation, but does not limit the scope of the present invention.

[0025] Cloning, identification and functional analysis of the full-length sequence of a new gene GhBZR1 in the cotton BZR family:

[0026] 1. Isolation and identification of GhBZR1 gene (cDNA) sequence

[0027] Using yeast two-hybrid technology, more than 200 target proteins of Gh14-3-3 protein were screened from cotton fibers, including GhBZR1. Through DNA and protein sequence analysis, using cotton fiber cell transcripts (total RNA), the full-length cDNA sequence of GhBZR1 was isolated and obtained, as shown in SEQ.ID.No.1. The amino acid sequence of the protein encoded by the gene sequence is shown in SEQ. ID.No.2 is shown.

[0028] 2. Quantitative RT-PCR analysis of GhBZR1 gene expression

[0029] (1) Total RNA extraction from cotton tissue (according to Li XB, Cai L, Cheng NH, Liu JW, 2002. Molecular characterization of the cot...

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Abstract

The invention relates to cloning and functional identification of a gene GhBZR1 for preferential expression of cotton fibers. The gene GhBZR1 is 1410bp in overall length and is coded with a protein containing amino acid 313. If a large amount of mRNA (messenger Ribonucleic Acid) of the gene GhBZR1 is accumulated in cotton fiber cells during a stretching development stage, the gene GhBZR1 is considered as a fiber preferential expression gene. The protein GhBZR1 is positioned in a cytoplasm and a cell nucleus, and is accumulatively increased in the cell nucleus after being induced by BL (Butyrolactone). The protein GhBZR1 can interact with a protein GhBIN2 and can be phosphorylated by the protein GhBIN2, thereby promoting the interaction carried out between the protein GhBZR1 and a protein 14-3-3. Thus, if the over-expression of the GhBZR1 is carried out in a mutant bri1 of arabidopsis BR, the scrubby phenotype of the arabidopsis BR can be restored. If the expression of the gene GhBZR1 in the cotton is inhibited by utilizing an RNAi (Ribonucleic Acid interfere) technique, the development of ovules and fibers of a transgenic cotton plant is apparently affected. Thus, the result speculates that the cotton fiber development and the fiber quality character are regulated by the gene GhBZR1 possibly in a manner of influencing a BR signal.

Description

Technical field [0001] The invention relates to the cloning and functional identification of a gene GhBZR1 that is predominantly expressed by cotton fiber. Background technique [0002] Cotton is the most important natural fiber crop in the world. my country is the world's largest cotton producer and cotton consumer. Cotton production occupies a very important position in my country's national economy. Cotton fiber is the main product of cotton production, and its output and quality directly determine the output value and benefits of cotton production. Therefore, improving the output and quality of cotton fiber has always been the main goal of cotton breeding. However, due to the negative correlation between the yield and quality of cotton fiber, it seriously hinders the simultaneous improvement of cotton fiber yield and quality. Traditional breeding methods are difficult to break the negative correlation between yield and quality, and new ways must be found to achieve simultane...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415
Inventor 李学宝周颖李晓洁李沫张则婷
Owner HUAZHONG NORMAL UNIV
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