A new strain of Poria cocos and its high-efficiency cultivation technique
A technology of Poria cocos and strains, which is applied in the field of selection and high-efficiency cultivation of new strains of Poria cocos, can solve the problems of decreased biological efficiency, backward cultivation technology, unsatisfactory industrial benefits, etc., and achieve reduction of harm and pollution of miscellaneous bacteria, Strong anti-miscellaneous bacterial infection ability and the effect of improving the survival rate of inoculation
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Embodiment 1
[0049] Example 1 Breeding method and character analysis of a new strain of Poria cocos (CCTCCNO: M2011072)
[0050] The breeding method of Poria cocos new strain (CCTCCNO: M2011072) of the present invention comprises the steps:
[0051] 1) Cultivation of mycelium: select wild Poria cocos with uniform and firm body, thin skin and good quality, and carry out tissue culture at 24-28°C to separate sclerotia and mycelium, wherein the tissue cultured The medium consists of 1000 ml of water, 200 grams of potatoes, 20 grams of glucose, 20 grams of agar, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 1.5 grams of vitamin B1 and 5 grams of peptone;
[0052] 2) Purification and rejuvenation of mycelium: Inoculate the separated mycelium into multi-tube culture medium, then place it in a sterile box, and cultivate it at 26-28°C for 7-10 days until the mycelium is fully grown. Select the one with the best growth condition to carry out transplantation culture, w...
Embodiment 2
[0056] Example 2 Study on the Efficiency of a New Strain of Poria Cocos (CCTCCNO: M2011072)
[0057] Promoted 523,000 bags of Poria cocos new strains (CCTCCNO: M2011072), cultivated 11.5 million bags of materials, created a total output value of 120.6086 million yuan, increased profits and taxes of 79.731 million yuan, and earned foreign exchange of 192,200 US dollars from exports. The total output value reached 116 million yuan, and generated Good ecological benefits, economic benefits and social benefits are obtained, and the results are shown in Table 1.
[0058] Table 1
[0059]
[0060]
[0061] 1. Economic benefits
[0062] Promoting 523,000 packs of Poria cocos strains (CCTCCNO: M2011072) of the present invention, its sales revenue can reach 1.046 million yuan, deducting costs of 627,600 yuan, and realizing profits and taxes of 418,400 yuan; among them, 523,000 packs of strains can plant 221,900 bags (cellar) of Poria cocos , each bag (cellar) raw material is ...
Embodiment 3
[0071] Example 3 Comparative study on high-efficiency bag material cultivation and section tree cultivation of a new strain of Poria cocos (CCTCCNO: M2011072)
[0072] The new strain of Poria cocos (CCTCCNO: M2011072) is cultivated with 100 bags of bag materials, and the average loading of each bag is 5kg, a total of 500kg; Cultivation techniques require cultivation management.
[0073] The research results of the bag material cultivation and section tree cultivation of the new Poria cocos strain (CCTCCNO: M2011072) were compared and studied. The minimum mass and maximum mass of single fresh Poria cocos, the total weight and physical and chemical cost of fresh Poria cocos harvested, etc., the results are shown in Table 3.
[0074] table 3
[0075]
[0076] As can be seen from Table 3, 100 bags of new strains of Poria cocos (CCTCCNO: M2011072) were cultivated in bags, and a total of 127 fresh Poria cocos were harvested. The minimum weight of a single Poria cocos was 0.25...
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