Method for acquiring peanut mutant by ion beam mutagenesis

Abstract

The invention relates to a method for acquiring a peanut mutant by ion beam mutagenesis. The method includes: using ion beams of different doses to irradiate different species of peanut embryos; subjecting the irradiated peanut embryos to water culture germination experiment, and subjecting M1 generation to field sowing; after harvesting of the M1 generation, treating and sowing M2 generation according to different species and different doses to obtain M3 generation peanut seeds; selecting the same number of field overground part plants from different species of mutagenesis groups, conducting single plant harvesting, performing airing, and then determining the fat content and fatty acid content so as to obtain a mutant. The method provided by the invention has the advantages that by means of ion beam mutagenesis, ion beam irradiation mutagenic treatment is carried out on different species of peanut embryos. A large number of experimental data researches show that ion beams enable peanuts to produce various mutants, but the effects are different among different species; mutant peanuts show traits different from normal plants at different growth and development stages, thus being conducive to selection and identification of peanut breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for obtaining peanut mutants through ion beam mutagenesis. Background technique [0002] As a physical mutagenesis method, ion beam mutagenesis has the advantages of small physiological damage, wide mutation spectrum, high mutation frequency, repeatability and directionality compared with traditional physical mutagenesis. way. [0003] The lack of excellent peanut germplasm resources has caused great constraints on the peanut breeding work. Physical and chemical mutagenesis can produce mutants, which has become an effective method to construct mutants; traditional physical mutagenesis can also produce mutagenesis, but there are low survival rate of M1 generation, narrow mutation spectrum of M2 generation, no directionality and breeding efficiency Inferior shortcomings, ion beam mutagenesis makes up for these shortcomings. [0004] In the 1920s, Muller discovered that X-ra...

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Problems solved by technology

However, an effective method for obtaining peanut mutants has not been disclosed in the prior art

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