Rapid identification molecular marker of self-compatible variety of loquat, marker primer and identification method

Abstract

The invention belongs to the field of molecular markers and provides a rapid identification method of a self-compatible Si molecular marker, a self-compatible marker primer and a self-compatible variety of loquat. The Si molecular marker is shown in a sequence SEQ ID NO. 1, and the size is 830bp; an AS-PCR (allele-specific polymerase chain reaction) sequence of the marker primer is that a forward primer is shown in SEQ ID NO. 2, and a reverse primer is shown in SEQ ID NO. 3. According to the identification method, by using Si as the molecular marker, DNAs of different loquat varieties are amplified by using the AS-PCR of the marker primer; the DNAs are screened and the DNAS where 830bp fragment can be amplified have the Si molecular marker, which shows that the loquat variety is the self-compatible variety. The method can quickly identify the self-compatible variety of loquat without arranging a pollinate tree or manual pollination, so that the cost can be greatly lowered; the method is hardly affected by external factors, is great in precision, breaks the limitation of conventional method, and has great significance on the yield and quality of loquat.

Description

technical field [0001] The invention belongs to the field of molecular markers, and relates to a loquat variety (line) self-compatibility S-RNase genotype S i Molecular markers, self-affinity marker primers and a rapid identification method for self-compatible varieties of loquat. Background technique [0002] Loquat (Eriobotrya Lind1.) belongs to the gametophytic self-incompatibility (GSI), genetically belongs to the multiple alleles (S-allele or S locus) of a single locus (S-locus, S locus). -gene, S gene) regulation, when the S allele of the pollen is the same as one of the S alleles in the style, the growth of the pollen tube in the style will be inhibited, so that the fertilization cannot be successfully completed and self-infertility will be shown. Affinity phenomenon seriously affects fruit yield. Therefore, in the cultivation, it is necessary to plant reasonable pollinated varieties or carry out artificial assisted pollination during the flowering period in order t...

AI Technical Summary

Problems solved by technology

[0006] However, the current understanding of the S genotype of the identified varieties is different, and the detection equipment and technical conditions are high, so that the progress of the identification of self-compatible varieties of loquat varieties has been very slow. Therefore, the identification of self-compatible varieties of loquat is a Research areas that are extremely weak and urgently need to be strengthened

Images