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Bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of bacillus methylothophicus strain

A methylotrophic, soft rot technology, applied in the field of microorganisms, can solve the problems of increasing the residual amount of toxic chemicals, polluting the ecological environment, pathogenic bacteria resistance, etc., to solve the problems of pesticide residues and environmental pollution, huge application potential, Easy-to-use effects

Inactive Publication Date: 2016-09-14
北京市辐射中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Every year in my country, the pear ring disease damages the production of pear fruit by about 25%, and the fruit rot rate is as high as 80% in severe cases, causing huge economic losses.
At present, chemical agents are often used in production to prevent and control postharvest diseases of pears, which not only seriously pollutes the ecological environment, but also increases the residual amount of toxic chemicals in agricultural products, which brings potential harm to human health and also easily induces the emergence of drug resistance of pathogenic bacteria

Method used

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  • Bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of bacillus methylothophicus strain
  • Bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of bacillus methylothophicus strain
  • Bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of bacillus methylothophicus strain

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Example 1, Isolation and Identification of Methylotrophic Bacillus Strain R1B

[0050] 1. Isolation and purification of strain R1B

[0051] 1. Rinse Dendrobium Huoshanense collected from Lu'an, Anhui with tap water, rinse with distilled water, dry and cut the roots, stems and leaves into small pieces for surface disinfection. The disinfection method is as follows: treat with 70% alcohol for 40-60 seconds, rinse with sterile water, then treat with 2.5% sodium hypochlorite solution for 2-5 minutes, and rinse with sterile water for 3-4 times.

[0052] 2. Under sterile conditions, take 100 μL of the sterile water produced by the last rinse and spread it on the beef extract medium, incubate at 30°C for 24-72 hours, observe whether there are colonies, and verify whether this disinfection method kills the bacteria. microorganisms on the surface of the test material.

[0053] 3. Under sterile conditions, cut the roots, stems and leaves of Dendrobium Huoshanense into 0.2cm 2 ...

Embodiment 2

[0070] Embodiment 2, the application of bacterial strain R1B in antibacterial or antibacterial

[0071] 1. Antagonistic activity of strain R1B against pear ringworm and soft rot bacteria

[0072] The pathogenic strains Botryosphaeria dothidea and Rhizopus stolonifer were respectively activated on the PDA medium, and the fungus cakes were punched out with a 6mm punch to obtain the cakes of Botryosphaeria dothidea and Rhizopus stolonifer respectively. Using the confrontation culture method, respectively inoculate the pear ringworm bacteria cake and soft rot bacteria cake on one side of a PDA plate with a diameter of 9 cm, and inoculate the strain R1B on the opposite side, with a distance of 3-4 cm between the two inoculation points, and inoculate separately The pathogenic strains on the PDA flat plate are the ringworm of pear and the soft rot fungus as a control. Set up 3 replicates, culture at 25°C, measure the diameter of the treated colony and the width of the bacteriostatic...

Embodiment 3

[0084] Embodiment 3, the application of bacterial strain R1B in the prevention and treatment of pear fruit ring spot

[0085] 1. Control effect of strain R1B on pear fruit ring spot

[0086] 1. Pear pretreatment

[0087] Select fruits that are neat in appearance, free of diseases and insect pests, and free of trauma, first rinse them with tap water, then spray them with 75% alcohol for disinfection, and dry the surface with sterile filter paper after 2 minutes for later use.

[0088] 2. R1B bacterial suspension treatment

[0089] Use a sterile puncher (diameter 6mm) to symmetrically punch two wounds with the same depth as possible on the surface of the fruit, and inoculate 50 μL of 5×10 7 CFU / ml of R1B bacterial suspension (R1B treatment group, R1B) and the same amount of sterile water (control group, ck), each treatment of 8 fruits, repeated 3 times, and placed at room temperature for one day.

[0090] 3. Inoculation of pathogenic bacteria

[0091] Inoculate R1B treatment...

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Abstract

The invention discloses a bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of the bacillus methylothophicus strain. The endophytic bacillus methylothophicus strain is separated from dendrobium huoshanense roots which are subjected to surface sterilization, and the strain has the broad-spectrum resistance on multiple plant pathogens and has the good prevention and control effect on pear fruit rot caused by the ring rot and the soft rot. The endophytic bacterium serving as a biocontrol bacterium has the advantages of the broad-spectrum bacterial resistance, the efficient safety and the like and is safe to pear fruits, low in large-scale production raw material, convenient to operate, stable in prevention and control effect and capable of effectively solving the problems of residual pesticides and environmental pollution which are brought by pesticide prevention and control over pear fruit diseases. Accordingly, the huge application potential on biological disease prevention and control after pear harvesting is achieved, and a good basic bacterial strain is provided for researching and developing microbial pesticides.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a methylotrophic bacillus for preventing and treating pear ring spot and soft rot and its application. Background technique [0002] Pear is one of the fruit trees with a long history of cultivation, large area and high yield in my country. According to statistics, pear is the third largest fruit in my country. At present, the total area of ​​the country is more than 1 million hectares, and the annual output is more than 8 million tons, accounting for 62% and 52% of the world's pear cultivation area and output respectively. During the growth and storage period of pear fruit, it will be threatened by diseases all the time. Among them, Botryosphaeria dothidea is one of the most serious fruit fungal diseases, and it is the main cause of pear fruit rot. Because the ringworm is a latent infection in the young fruit stage, it will develop during the ripening and storage of th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A23B7/155C12R1/07
CPCA01N63/00A23B7/155C12N1/205C12R2001/07
Inventor 武利勤尚宏忠顾海科王青刘桂君周思静王平
Owner 北京市辐射中心
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