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A kind of Huoshan Dendrobium seedling propagation method

A technology for Dendrobium huoshanense and seedlings is applied in the field of efficient propagation of Dendrobium huoshanense seedlings, which can solve the problems of small number of plants, narrow habitat, and propagation of Dendrobium huoshanense, and achieve the effects of high survival rate, low cost and simple operation.

Active Publication Date: 2011-12-21
安徽康顺名贵中草药产业开发有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its narrow habitat, difficult reproduction, and very few plants, the artificial cultivation of Dendrobium Huoshanense has broad prospects, but the lack of high-quality seedlings is one of the bottlenecks in the cultivation of Dendrobium Huoshanense
Although there have been patents and literature reports on the production of Dendrobium Huoshanense seedlings in China, there has been no use of the method of flowering and pollination results in test tubes to propagate Dendrobium Huoshanense

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Acquisition of explants: Select a robust mother plant for artificial pollination during flowering, and the fruit will mature 120 days after pollination, and the fruit will be used as an explant for sowing;

[0050] 2. Protocorm induction: When sowing, the explants are soaked in alcohol with a volume fraction of 75% for 30 seconds, sterilized in a mercury solution with a mass fraction of 0.2% for 30 minutes, rinsed with sterile water for 5 times, and then cut the fruit. The powdered embryos are inoculated on the seed germination medium KnP1, and the seeds germinate 10 days later to form protocorms, and 70 days later to form seedlings;

[0051] 3. Artificial pollination: Transfer 100 seedlings with a height of 2.5 to 3.5 cm to the test-tube flowering medium KnP2. After 58 days, 21 plants will bloom, and the flowering rate can reach 21%. Select the plants that grow robustly in the test tube for artificial pollination in the test tube. ;

[0052] 4. Aseptic sowing: 70 d...

Embodiment 2

[0057] 1. Preparation of explants: Select a robust mother plant for artificial pollination during flowering, and the fruit is ripe 130 days after pollination, and the fruit is used as an explant for sowing;

[0058]2. Protocorm induction: When sowing, soak the explants in alcohol with a volume fraction of 75% for 30 seconds, sterilize them in a mercuric chloride solution with a mass fraction of 0.2% for 30 minutes, rinse with sterile water for 5 times, and then cut the fruit. Use an inoculation needle to inoculate the yellow-white powdery embryos onto the seed germination medium KnP1, after 10 days the seeds germinate and form protocorms, in order to obtain more seedlings during the production process, mix protocorm-forming buds and protocorm-like tissue, subcultured on the subculture proliferation medium KnP1, multiplied by 4 times after 40 days, and the formed protocorm continued to proliferate to obtain rooted seedlings, and the number of seedlings obtained for each fruit wa...

Embodiment 3

[0065] 1. Preparation of explants: Select a robust mother plant for artificial pollination during flowering, and the fruit is ripe 140 days after pollination, and the fruit is used as an explant for sowing;

[0066] 2. Protocorm induction: When sowing, soak the explants in alcohol with a volume fraction of 75% for 30 seconds, sterilize them in a mercuric chloride solution with a mass fraction of 0.2% for 30 minutes, rinse with sterile water for 5 times, and then cut the fruit. Use an inoculation needle to inoculate the yellow-white powdery embryos onto the seed germination medium KnP1, after 10 days the seeds germinate and form protocorms, in order to obtain more seedlings during the production process, mix protocorm-forming buds and protocorm-like Tissues, subcultured on the subculture medium KnP1, multiplied by 4 times after 40 days, the formed protocorm continued to proliferate, and rooted seedlings were obtained, and the number of each fruit was more than 40,000;

[0067] ...

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Abstract

The invention relates to a method for propagating dendrobidium huoshanness seedlings, which comprises the following steps: preparing explants, performing sterile seed sowing, inducing protocorms, performing artificial pollination, culturing strong seedlings, transplanting test tube seedlings and the like. In the method, strong flowering dendrobidium huoshanness plants are used as mother plants and pollinated artificially to grow fruits, seeds are collected from mature fruits and cultured in a seed germination culture medium to grow protocorms and consequently seedlings, the seedlings are cultured in a flowering culture medium in test tubes, the flowering seedlings are pollinated artificially under a sterile condition, seeds are collected from mature fruits and sowed in a sterile manner, the seeds are grown into small plants, namely seedlings that can be transplanted from the test tubes, and transplanting the seedlings from the test tubes. When the method is used, the operation is simple, the cost is low, the maturing period of the fruit of dendrobidium huoshanness is shortened, the efficiency is high, diseases and insects are prevented, dendrobidium huoshanness seeds can be produced all the year round, the transplantation survival rate of the grown seedlings may reach over 96 percent, and a new approach is provided for propagating dendrobidium huoshanness seedlings.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to the achievement of Dendrobium Huoshanense (Dendrobium huoshanense) by test tube flowering and artificial pollination results. Dendrobium huoshanense C. Z. Tang et S. J. Cheng ) method for efficient propagation of seedlings. Background technique [0002] Huoshan Dendrobium ( Dendrobium huoshanense C. Z. Tang et S. J. Cheng ) is a rare species on the verge of extinction in my country. It is known as the first of the "Chinese Immortal Herbs". It is a unique and precious Chinese medicinal material with geographical indications of origin in Huoshan County. , heat-clearing and nourishing yin, anti-inflammatory and pain-relieving, voiceless and eye-improving functions and anti-cancer, anti-aging, anti-radiation and immunity-enhancing effects, especially Dendrobium polysaccharides have extremely high health care and medical effects. However, due to its narrow habitat, difficult repr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 汤海荣
Owner 安徽康顺名贵中草药产业开发有限公司
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