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Method for rapid tissue culture propagation of Dendrobium huoshanense seedlings

A technology of Huoshan Dendrobium and Huoshan Stone, which is applied in the field of rapid tissue culture and propagation of Huoshan Dendrobium seedlings, can solve the problems of cumbersome operation, high cost, and many steps, and achieve the effects of simple operation, high germination rate, and low cost

Inactive Publication Date: 2013-11-20
哈福科技(武汉)集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] The object of the present invention is, to carry out research and improvement for the problems of many steps, cumbersome operation and high cost faced in the above-mentioned Dendrobium huoshanense tissue culture propagation, and propose a rapid tissue culture propagation method of Dendrobium huoshanense seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The seeds and culture medium of Dendrobium huoshanense are used to propagate and cultivate the seedlings, and the propagation and cultivation of the seedlings includes two steps of sowing germination and cultivating strong seedlings.

[0034] Sowing and germination steps: take mature Dendrobium huoshanense capsules, add 0.1% HgCl 2 After soaking in the solution for 10 min, rinse with sterile water 3 times. The seeds in the mature Dendrobium Huoshanense capsules soaked in the solution and washed clean are taken out, the pericarp of the mature Dendrobium Huoshanense capsules is cut open with a scalpel, and the seeds of Dendrobium Huoshanense are taken out therefrom, and the seeds of the Dendrobium Huoshanense taken out are evenly sown on the On the pre-prepared medium, the medium used is the germination medium. The composition of the germination medium: MS medium is used as the base medium, and the plant growth regulator - indole acetic acid IAA, and zeatin ZT (6-trans-...

Embodiment 2

[0040] The seeds and culture medium of Dendrobium huoshanense are used to propagate and cultivate the seedlings, and the propagation and cultivation of the seedlings includes two steps of sowing germination and cultivating strong seedlings.

[0041] Sowing and germination steps: take mature Dendrobium huoshanense capsules, add 0.1% HgCl 2 After soaking in the solution for 10 min, rinse with sterile water 3 times. The seeds in the mature Dendrobium huoshanense capsules soaked in the solution and washed clean are taken out, the pericarp of the capsules is cut open with a scalpel, the seeds are taken out, and the seeds are evenly sown on the pre-prepared medium. The base is the germination medium. The composition of the germination medium: MS medium is used as the base medium, and the plant growth regulators-indole acetic acid IAA and zeatin ZT (6-trans-4-hydroxy-3-methyl-but-2-ene aminopurine), combined with potato extract and agar to form a germination medium. In the germin...

Embodiment 3

[0046] The seeds and culture medium of Dendrobium huoshanense are used to propagate and cultivate the seedlings, and the propagation and cultivation of the seedlings includes two steps of sowing germination and cultivating strong seedlings.

[0047] Sowing and germination steps: take mature Dendrobium huoshanense capsules, add 0.1% HgCl 2 After soaking in the solution for 10 min, rinse with sterile water 3 times. The seeds in the mature Dendrobium Huoshanense capsules soaked in the solution and washed clean are taken out, the capsule skin is cut open with a scalpel, the seeds are taken out, and the seeds are evenly sown on the pre-prepared medium. The medium used is Germination medium. The composition of the germination medium: MS medium is used as the base medium, and the plant growth regulators-indole acetic acid IAA and zeatin ZT (6-trans-4-hydroxy-3-methyl-but-2-ene aminopurine), combined with potato extract and agar to form a germination medium. In the germination med...

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Abstract

The invention relates to a method for rapid tissue culture propagation of Dendrobium huoshanense seedlings. The seeds and medium of Dendrobium huoshanense are employed to breed seedlings. Breeding of the seedlings consists of the steps of sowing germination and strong seedling culture. The seeds of Dendrobium huoshanense are adopted as the source of seedling breeding, and are acquired from mature Dendrobium huoshanense capsules. In the sowing germination step, the medium is employed to germinate the seeds of Dendrobium huoshanense, the acquired seeds of Dendrobium huoshanense are uniformly sowed in the medium well prepared in advance. The Dendrobium huoshanense's seeds entering the medium absorb nutrients supplied by the medium to make the seeds of Dendrobium huoshanense germinate rapidly. By means of the sowing germination step, the seeds of Dendrobium huoshanense grow into Dendrobium huoshanense seedlings with stems and leaves. The Dendrobium huoshanense seedlings with stems and leaves then grow into plantlets through the strong seedling culture step, thereby forming the seedlings able to be planted out of bottles. The method provided in the invention has the advantages of reasonable design, method optimization, simple operation, and low cost.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food, and in particular relates to a rapid tissue culture propagation method for Dendrobium huoshanense seedlings. Background technique [0002] Dendrobium, orchidaceae Dendrobium ( Dendrobium ) is a perennial herb, which is recorded in many classical medical books such as "Shen Nong's Materia Medica", "Dao Zang", "Compendium of Materia Medica", "Prescriptions for Emergencies", and is a traditional Chinese medicine in my country. [0003] Modern research shows that the active components of Dendrobium mainly include chemical components such as alkaloids, amino acids and polysaccharides. Pharmacological studies have shown that Dendrobium has significant curative effects in improving human immunity, anti-aging, anti-tumor, improving symptoms of diabetes, and treating atrophic gastritis. Very obvious. But dendrobine can inhibit respiration. [0004] As far as the applicant knows, Dendr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何祥久徐联武
Owner 哈福科技(武汉)集团有限公司
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