The invention relates to the technical field of medicine, and discloses a cloxacillin sodium liposome preparation for injection and a preparation method thereof. The cloxacillin sodium liposome preparation is a powder injection, and is a sterile nano freeze-drying preparation which is prepared by coating salmon calcitonin with a pharmaceutically-acceptable biological carrier. The cloxacillin sodium liposome preparation comprises the following raw materials in parts by weight: 1 part of salmon calcitonin, 4-15 parts of pharmaceutically-acceptable biological carrier, 0.5-2 parts of stabilizer and 2-10 parts of freeze-drying protective agent. The product disclosed by the invention has high stability and a low side effect, can not be cracked as a result of dehydration, fusion, generation of ice crystals and the like in the freeze-drying process, and can keep a good entrapment rate after hydration and refusion, thereby facilitating the transportation and storage of the product.