Hucho trout biological slice making technology

A production technology, the technology of jellyfish, which is applied in educational utensils, instruments, teaching models, etc., can solve the problems of incomplete production, unclear reagents, harmful and other problems, achieve fresh dyeing, reduce environmental pollution, acid and alkali Cell distinct effect

Inactive Publication Date: 2011-09-14
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a biological slicing technology for cymbals, the purpose of which is to solve the problems of incomplete slicing, unclear visibility after sealing and harmful reagents in the existing conventional biological slicing technology

Method used

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  • Hucho trout biological slice making technology
  • Hucho trout biological slice making technology
  • Hucho trout biological slice making technology

Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0011] Specific embodiment one: the biological preparation technology of cymbidium japonicus in this embodiment is carried out according to the following steps: one, the cyprus cymbidium sample adopts Bouin's solution to fix 2h, then rinses 10min with running water, then places in A solution, B solution and pine successively 30 min each in terpineol; 2. Take out the terpineol samples and place them in a mixture of equal volumes of terpineol and paraffin for 30 to 40 min. After taking them out, place them in paraffin for 2 hours for embedding. Slice after cooling at room temperature; 3. Stain the slices with HE (hematoxylin-eosin staining method), and then seal the slices with Euparal (English translation into Chinese: Euparal), that is, complete the biological preparation of the salmon;

[0012] Wherein in step one, the sample of the crocodile is the various tissues of the crocodile; in the step one, the first solution is composed of 6 parts of absolute ethanol, 3 parts of chlo...

specific Embodiment approach 2

[0015] Specific embodiment two: the difference between this embodiment and specific embodiment one is that Bouin's solution in step one is made up of the formaldehyde of 25ml, the picric acid saturated solution of 75ml and the glacial acetic acid of 5ml. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0016] Specific embodiment three: the difference between this embodiment and specific embodiment one is that step one is placed in terpineol for 30 minutes, and the terpineol must be replaced once in the middle. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a hucho trout biological slice making technology, and relates to a slice making technology. The technology of the invention solves the problems existing in the conventional slice making technology. The problems are that the slice is incomplete, the slice is not clear after slice sealing, and the agent is harmful. The making method includes the steps of: (1) fixing a hucho trout sample by employing the Bouin's solution, washing the sample by running water, and placing the sample into a first liquid, a second liquid, and terpineol in turn; (2) taking out the sample from the terpineol and placing into a mixed liquid which has equal volumes of terpineol and paraffin, and then placing the sample into the paraffin for embedding, slicing the sample after cooling; (3) carrying out the HE dyeing to the slice, and obtaining a finished product after sealing the slice by Euparal. The hucho trout glass sample obtained through the hucho trout biological slice making technology has the characteristics of fresh dyeing color, clear acid and alkaline cells, complete slice composition, clear image, and well-defined outline. The making technology of the invention saves many steps compared with the conventional making method, and reduces environmental pollution.

Description

technical field [0001] The invention relates to a tablet making technology. Background technique [0002] At present, the conventional biofilming technology adopted is to use benzene alcohol as the transparent agent and the organic solvent of paraffin. Some biological materials subjected to the action of benzene alcohol will shrink, deform, harden and become brittle, and cannot be made into a complete film, resulting in The experiment cannot be continued, and benzene substances have toxic effects on human hematopoietic organs, nervous system, and digestive system. For example, repeated exposure to benzene for a long time can even paralyze the central nervous system and cause environmental pollution; The slide technology uses neutral gum as the mounting agent, but there is a problem that the slide cannot be clearly seen after the slide is mounted. The existing conventional biological production technology is poorly targeted and lacks a production technology for a single spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36
Inventor 关海红尹家胜
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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