46 results about "Edwardsiella sp." patented technology

The present invention provides a turbot-derived Edwardsiella piscicida and its application; the Edwardsiella piscida derived from turbot is Edwardsiella piscida H4-S18 strain, and its gene sequence is as shown in SEQ ID No. .1. Shown in SEQ ID No.2 and SEQ ID No.3, the Edwardsiella piscicidae H4‑S18 strain is preserved with the preservation number CCTCC NO: M 2020450. The present invention also relates to the application of the Edwardsiella piscicida derived from turbot in the preparation of vaccines. The piscicidal Edwardsiella provided by the present invention is screened from the diseased turbot, and it is the first screening of the turbot-derived Edwardsiella piscicida carrying the drug-resistant gene in China, and the first application of the piscicidal Edwardsiella carrying the drug-resistant gene. Vaccines prepared from turbot-derived Edwardsiella piscicida can effectively improve the immune protection rate of turbot against drug-resistant Edwardsiella piscicida.

The invention discloses a yellow catfish β-defensin gene and its β-defensin antibacterial peptide and its application; the invention successfully expresses the yellow catfish β-defensin recombinant protein PET‑32a‑Pf_BD using in vitro recombinant expression technology, and inhibits Bacterial activity identification results show that it has a certain antibacterial effect on Staphylococcus aureus in Gram-positive bacteria, Escherichia coli in Gram-negative bacteria, Aeromonas hydrophila, Edwardsiella and Flavobacterium columnar. effect. This laid the foundation for the application of defensin recombinant protein as a broad-spectrum antibacterial drug in aquaculture.

The invention relates to a marker-free gene deletion attenuated mutant strain of a Edwardsiella tarda wild strain. The marker-free gene deletion attenuated mutant strain is an attenuated live vaccine of an Edwardsiella tarda virulent strain, which deletes the chorismic acid synthase gene aroC of the Edwardsiella tarda virulent strain, three types of secretion system response element genes of eseB, escA, eseC and eseD and an endogenous plasmid, preferably, the Edwardsiella tarda virulent strain is a Edwardsiella tarda virulent strain EIB202 with the preservation number of CCTCC No:M208068; the endogenous plasmid is a plasmid of pEIB202; and the marker-free gene deletion attenuated mutant strain of the Edwardsiella tarda virulent strain is an attenuated strain WED with the preservation number of CCTCC No:M2010278. The invention also provides relevant preparations and application of the marker-free gene deletion attenuated mutant strain. The attenuated mutant strain or relevant preparations eliminate the potential environment and safety risk of products existing in the traditional attenuated live vaccines generally and is a safe, effective and economic vaccine aiming at Edwardsiella tarda diseases of cultured fishes.

The invention relates to the field of molecular immunology, in particular to an Edwardsiella tarda surface display type vaccine and preparation and application thereof. The surface display type vaccine is shown as a base sequence in a sequence table SEQ ID No.1. The preparation method comprises the following steps of: performing polymerase chain reaction (PCR) by using a primer F1 / R1 and by taking Edwardsiella tarda TX1 as a template; and connecting a PCR product with a plasmid pBT3, and transforming Escherichia coli by using connection liquid to obtain a coded vaccine strain of Edwardsiella tarda vaccine protein for expressing the base sequence in the sequence table SEQ ID No.1. The vaccine strain of the Edwardsiella tarda vaccine protein coded by the base sequence in the sequence table SEQ ID No.1 has the effect of inhibiting Edwardsiella tarda. The vaccine is easy to prepare, is not required to be subjected to any processes such as extraction, purification and inactivation, and does not need any adjuvant when used.

The invention relates to the technical field of molecular biology, in particular to a method for quickly identifying sRNA of Edwardsiella piscicida. The method provided by the present invention comprises: constructing the hfq gene deletion mutant strain of Edwardsiella piscicida; detecting the expression levels of sRNA in the wild strain and the hfq mutant strain respectively by using fluorescent quantitative PCR, and judging the difference between the sRNA and Hfq according to the difference in the expression levels of the two. dependencies between proteins. The method provided by the present invention can quickly and accurately determine the dependence relationship between sRNA and Hfq protein, the operation steps are simple, no technically difficult experimental operations such as RNA co-immunoprecipitation are required, and high-throughput detection and identification can be realized, greatly reducing the The detection cost is easy to be widely used.

The invention relates to the field of molecular biology, in particular to the application of a serine protease (PoCFI-Tryp) of a flounder complement regulatory protein I factor. The application of serine protease (PoCFI‑Tryp) in the complement regulatory protein I factor of flounder in the preparation of bacteriostatic agents. The serine protease of the present invention can be combined with various bacteria, and can significantly inhibit the growth of Edwardsiella lentus, Vibrio eel, Vibrio harvey and Streptococcus iniae. The obtained protein has application potential in anti-bacterial infection.

The invention relates to the field of molecular vaccinology, in particular to an Edwardsiella tarda outer membrane protein vaccine and its preparation method and application. The outer membrane protein vaccine contains the Edwardsiella tarda outer membrane protein vaccine antigen shown by the amino acid in SEQ ID No.1. The recombinant protein vaccine constructed by the invention can protect the flounder against the infection of Edwardsiella tarda, induce the production of specific antibodies, and the protein can significantly promote the immune activity of fish immune cells.

The invention discloses a freeze-vacuum drying protection agent for Edwardsiella tarda and a freeze-drying method thereof, belonging to the technical field of freeze-vacuum drying preservation of marine bacteria. The ingredients are: skimmed milk powder, lactose, trehalose, vitamin C, NaCl and sterile water. Under aseptic conditions, mix the live bacterial suspension of Edwardsiella tarda and the above-mentioned freeze-drying protectant solution in a volume ratio of 1:4 to 1:1, divide them into separate packages, and quickly freeze them at -80°C After 5 to 6 hours, freeze-dry for 18 hours at a vacuum degree of 0.28mbar and a freeze-drying chamber temperature of -54°C for 18 hours, then reduce the vacuum degree to 0.034mbar and continue to freeze-dry for 2 hours at a temperature of -54°C. After the freeze-drying is completed, fill the freeze-drying cabin with 99.99% dry carbon dioxide gas to standard atmospheric pressure and seal the cover. The invention establishes a technical method for long-term preservation of Edwardsiella tarda by freeze-drying and vacuum drying, and provides technical support for in-depth related research on Edwardsiella tarda.