Novel edwardsiella attenuated vaccine strain as well as preparation method and application thereof

Abstract

The invention relates to a novel edwardsiella attenuated strain, wherein the expression of a target gene or a protein coded by the target gene is regulated and controlled. The edwardsiella attenuated strain can be used as a preventive drug for edwardsiella diseases, and has extremely obvious effect on inhibiting edwardsiella infection.

Description

technical field [0001] The invention belongs to the field of immunology, and more specifically, the invention relates to a novel attenuated vaccine strain of Edwardsiella, its preparation method and application. Background technique [0002] Edwardsiella can cause Edwardsiella, which is an important fish pathogen and can cause disease to more than 20 kinds of freshwater and seawater fish such as catfish, turbot and eel, causing gastrointestinal inflammation and hemorrhagic sepsis, resulting in A large number of fish die-offs seriously threaten the aquaculture industry. [0003] With the continuous expansion of aquaculture farming scale, large-scale outbreaks of aquaculture diseases often occur. Fish infectious diseases account for more than 20% of production losses every year, and large-scale disease outbreaks have become a major obstacle for the aquaculture industry. The main causes of fish infectious diseases include bacteria, parasites and viruses. [0004] Edwardsiell...

AI Technical Summary

Problems solved by technology

[0005] At present, chemically synthesized drugs or antibiotics are generally used in marine aquaculture production to control the large-scale outbreak of the disease, but there are many disadvantages in long-term drug use: 1) Sometimes the cause of fish diseases is complex, and there is blindness in a large number of drugs. At present, there is no targeted drug to treat; 2) The preventive effect of chemical drugs is very weak, and the sick fish eat poorly, and cannot ingest effective drug doses; 3) The drugs ingested by fish often remain in them, endangering human health, and Long-term drug use will bring environmental pollution and lead to the emergence of a large number of microbial resistance

Moreover, under the maintenance of long-term drugs, a large number of individuals with weak disease resistance that should have been eliminated survived, not only affecting the quality of fish meat, but also reducing the disease resistance of the entire population, seriously affecting the development of the mariculture industry

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