Method for identifying salt tolerance of cotton

An identification method and a technology of salt tolerance, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long identification time, large floor area, and low efflux capacity, so as to reduce the pre-cultivation time, The effect of reducing errors and avoiding errors

Inactive Publication Date: 2012-07-18
SHANDONG COTTON RES CENT
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Problems solved by technology

In 2011, Cuin et al. used non-damaging micro-measurement technology to study and found that Na in the roots of salt-tolerant wheat cultivars under salt stress + The efflux capacity is higher than that of salt-sensitive wheat cultivars, but K + Efflux capacity is lower than that of salt-sensitive wheat varieties
At present, the identification of cotton salt tolerance generally adopts methods such as salt ponds, potted plants, and seawater irrigation to measure cotton biomass and Na after salt treatment. + 、K + Physiological indicators such as content, this method not only occupies a large area, takes a long time to identify, but also usually requires destructive sampling, and the effect is sometimes unstable

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  • Method for identifying salt tolerance of cotton
  • Method for identifying salt tolerance of cotton

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Embodiment 1

[0019] Get 3 sterilized petri dishes with a radius of 10 cm, put them into two filter papers of the same size respectively, and add 10 ml of sterilized double distilled water; put Si Mian No. 3 (SM3) and two strains of overexpressed mountain spinach CMO gene 20 develvet seeds of salt-tolerant cotton strains (CMO2 and CMO4) were soaked, peeled off the seed coats, sterilized with 0.1% mercuric chloride solution for 15 minutes, and then washed 6 times with sterilized double distilled water. In a dish, put it in a greenhouse (22-32°C) to germinate and emerge. After the cotyledons of the cotton seedlings were fully unfolded, 10 robust cotton seedlings of the same size were selected for each variety and placed in a seedling pot filled with Hoagland nutrient solution (22-32°C, humidity 60%-75%). Change every day. After the cotton grew one true leaf, three cotton seedlings with intact roots and the same size were selected from each line and treated with Hoagland nutrient solution con...

Embodiment 2

[0024] Take 2 sterilized petri dishes with a radius of 10 cm, put them into two filter papers of the same size, and add 10 ml of sterilized double distilled water; put the plump cotton (Lumianyan No. 28) and seaside mallow seeds 20 capsules each, peeled off the seed coat, soaked in 0.1% mercuric chloride solution for 15 minutes, then washed 6 times with sterilized double-distilled water, planted in the above-mentioned petri dishes respectively, and placed in a greenhouse (22-32°C) to germinate and emerge. After cotton (Lumianyan No. 28) and mallow cotyledons are fully expanded, select 10 strong seedlings of the same size and put them into a seedling pot filled with Hoagland nutrient solution (22~32°C, humidity 60%~75%) ), the nutrient solution was changed every two days. After one true leaf grew, three seedlings with complete root system and the same size were selected and treated with Hoagland nutrient solution containing 150 mM NaCl for 24 hours.

[0025] Table 2 Na in root...

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Abstract

The invention discloses a method for identifying salt tolerance of cotton, comprising the following steps: selecting a robust cotton seedling with one true leave, treating the cotton seedling with a nutrient solution containing 150mM of NaCl for 24h, selecting a lateral root in a good growth state, cutting off the point of the lateral root, pre-treating the point of the lateral root in a test buffer solution for 30min, fixing the point of the lateral root in a culture dish filled with a fresh test solution, testing the point of the lateral root by using a non-invasive micro-test system, analyzing data by using Mageflux software to obtain an efflux velocity of Na<+> and an efflux velocity of K<+>, and calculating to obtain an efflux velocity ratio of Na<+> / K<+>. The higher the efflux velocity of the Na<+>, the lower the efflux velocity of the K<+>, or the higher the efflux velocity ratio of the Na<+> to the K<+>, the higher the salt tolerance of the cotton. The method for identifying the salt tolerance of the cotton has the characteristics of non-invasive detection, accurate data and rapid identification, can be used for identifying the salt tolerance of cotton and can be also used for salt tolerance of other plants.

Description

technical field [0001] The invention relates to a method for identifying the salt tolerance of cotton, specifically measuring the Na in cotton roots under salt stress by using a non-damaging micro-measurement technique. + and K + Flow rate, a method for identifying salt tolerance in cotton. Background technique [0002] In 1974, the neuroscientist Jaffe of the American Marine Biology Laboratory first proposed the original concept of non-invasive micro-measurement technology. In 1990, the non-invasive micro-measurement technology was successfully applied to the determination of Ca in cells. 2+ At present, this technology has been widely used in the fields of life science, environmental science and material science. Ca 2+ 、H + 、K + 、Na + , Cl - The information of plasma flow velocity and three-dimensional motion direction has the characteristics of living body, dynamic, real-time, internal and external measurement, long-term, multi-dimensional scanning and measurement. ...

Claims

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Application Information

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IPC IPC(8): A01G7/00
Inventor 董合忠孔祥强罗振代建龙李维江辛承松唐薇张冬梅
Owner SHANDONG COTTON RES CENT
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