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Cell culture medium for preparing human skin flbroblast sheet and using method of cell culture medium

A technology of fibroblasts and cell membranes, applied in the direction of cell culture active agents, biochemical equipment and methods, epidermal cells/skin cells, etc., which can solve problems such as easy loss of injection

Inactive Publication Date: 2017-05-10
陕西九州生物医药科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention utilizes the extracellular matrix components rich in collagen and elastin secreted by fibroblasts during the culture process as natural scaffold components to prepare fibroblast membranes, which ensures the activity of fibroblasts and solves the problem of Solved the problem of easy loss of fibroblast injection

Method used

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  • Cell culture medium for preparing human skin flbroblast sheet and using method of cell culture medium
  • Cell culture medium for preparing human skin flbroblast sheet and using method of cell culture medium
  • Cell culture medium for preparing human skin flbroblast sheet and using method of cell culture medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Culture medium preparation

[0066] 1. Basal medium

[0067] Basal medium: H-DMEM (High Glucose Dulbecco's Modified Eagle Medium), or DF12;

[0068] Form: dry powder (pure water, filtered), liquid;

[0069] Supplied Status: Sterile

[0070] Configuration method: Add a part of sterile ultrapure water to the basic medium powder (such as DMEM), dissolve it first, and then make constant volume according to the volume specified in the medium manual. After fully dissolving, filter and seal with 0.22 micron filter membrane. Then add 5%-15% serum to the basal culture medium as required.

[0071] If it is liquid DMEM, directly add 5%-15% serum to the container and mix well.

[0072] pH adjustment: Use pH test paper (or pH potentiometer) to test the pH value of the medium. If it does not meet the requirements, it can be adjusted with 10% HCl or 10% NaOH until it meets the formulation requirements, generally between pH 7.0-7.5.

[0073] The formulation of H-DMEM is shown ...

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Abstract

The invention discloses a cell culture medium for preparing a human skin flbroblast sheet. The cell culture medium comprises the following components: (1) a basic culture medium, namely, H-DMEM (high glucose dulbecco's modified eagle medium) or DMEM-F12; (2) an additive component, namely, vitamin C 50-100 [mu]g / ml, and epidermal growth factors (EGF) 3-20 ng / ml, and (3) serum 5-15%. In the fibroblast basic culture medium, other components are added, so that the cultured fibroblasts form a sheet. The invention further discloses a using method of the cell culture medium.

Description

technical field [0001] The invention relates to a cell culture medium, in particular to a cell culture medium for preparing a human skin fibroblast membrane sheet and a method for using it, belonging to the field of biotechnology. Background technique [0002] Fibroblasts are the main cells that make up the dermis of the skin. They are the main cells that produce collagen, elastin and other ECM (extracellular matrix) in the dermis. The role of collagen and elastin in skin tissue is to form skin scaffolds. Maintain skin elasticity. Absorb moisture, make skin delicate, smooth and elastic. [0003] Therefore, the normal proliferation and differentiation of fibroblasts maintain the normal structure and physiological functions of the skin. With age, the thickness of the dermis of the skin becomes thinner, and the number of fibroblasts in the dermis decreases, which leads to a series of skin problems: wrinkles, loss of elasticity, relaxation, dryness and lack of luster. The roo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N2500/38C12N2501/11
Inventor 聂苏秦
Owner 陕西九州生物医药科技集团有限公司
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