Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells

A technology of human pluripotent stem cells and a culture method, which is applied in the field of human pluripotent stem cell culture medium and human pluripotent stem cell culture, can solve the problems of affecting the extra-expansion efficiency of human pluripotent stem cells and easily generating human body rejection, and achieves Stable quality, clear ingredients, high growth efficiency

Active Publication Date: 2014-07-30
BEIJING CELLAPY BIOTECH
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a culture medium for human pluripotent stem cells (hES/iPS) and a method for culturing human pluripotent stem cells, so as to solve the problem in the prior art that human pluripotent stem cells (hES/iPS) are exposed to rich ani

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells
  • Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells
  • Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells

Examples

Experimental program
Comparison scheme
Effect test
No Example Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a human pluripotent stem cell culture medium and a culture method of human pluripotent stem cells. The human pluripotent stem cell culture medium comprises a basic culture medium and additives, wherein the basic culture medium is a DMEM/F12 (Dulbecco Modified Eagle Medium); the additives comprise 400-600mu g/ml sodium bicarbonate, 8-20ng/ml sodium selenate, 10-30ng/ml recombinant human insulin growth factor, 80-120ng/ml recombinant human alkali fibroblast growth factor, 7-13mu g/ml recombinant human lactoferrin, 50-70mu g/ml ascorbic acid and 1-3ng/ml recombinant human transforming growth factor. The culture medium disclosed by the invention is definite in component, stable in quality and free of animal resource components, in the culture process, no animal source cells are needed to be used as a feeder layer, cultured hES cells and hips cells are less in possibility of having human rejection reaction after being transplanted, and the growth efficiency is high.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a human pluripotent stem cell (hES / iPS) culture medium and a method for culturing the human pluripotent stem cell. Background technique [0002] In 1998, Thomson (Thomson et al., 1998) of the University of Wisconsin in the United States first successfully isolated human embryonic stem cells (hES cells) using the blastocyst inner cell mass from artificial in vitro fertilization (in vitro ferlilization, IVF). And cultivate the establishment. Cells have a high capacity for self-renewal and can expand infinitely under in vitro culture conditions. At the same time, hES cells can also differentiate into various cells derived from the three germ layers under certain conditions. Therefore, hES cells are widely used in the research of regenerative medicine, drug screening and embryonic development biology, and have been a research hotspot since its establishment. [0003] In 2006 a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/074
Inventor 李扬兰峰李厚鹏
Owner BEIJING CELLAPY BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com