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Human mycoplasma pneumoniae culture medium and diagnosis kit thereof

A technology of mycoplasma pneumoniae and culture medium, applied in the direction of microorganism-based methods, microorganism measurement/inspection, microorganisms, etc., can solve the problems of high cost, easy pollution, and tediousness, and achieve special physiological and biochemical effects, obvious effects, and good selectivity Effect

Active Publication Date: 2015-06-03
众爱生河北生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The traditional methods of isolation, culture and identification of difficult-to-culture bacteria are complex, cumbersome, easy to pollute, and expensive

Method used

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  • Human mycoplasma pneumoniae culture medium and diagnosis kit thereof
  • Human mycoplasma pneumoniae culture medium and diagnosis kit thereof
  • Human mycoplasma pneumoniae culture medium and diagnosis kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Human Mycoplasma pneumoniae liquid culture medium preparation:

[0042]

[0043] Formulation and packaging:

[0044]1. According to the quantity of medium to be prepared, weigh the reagents and mix thoroughly (such as solid medium plus agar 10-15g).

[0045] Wherein, the preparation method of poultry egg yolk extract is as follows: (1) take fresh selenium-enriched eggs and soak them in 70v / v% ethanol solution for 30 minutes; In a sterile flask; (3) Add 90v / v% ethanol to the sterile flask containing egg yolk in a weight ratio of 1:2, stir and mix well, seal the bottle and place it at 4°C to 8°C, Store for 20-24h; (4) Use 1N NaoH to adjust the pH of the solution after step (3) to 7.0-8.0, and centrifuge at 6000RPM to take the supernatant, and filter the supernatant aseptically with a 0.45μm filter membrane, and the filtrate It is poultry egg yolk extract, and the filtrate is aseptically divided into separate packages for later use.

[0046] 2. Then add phenol red in...

Embodiment 2

[0054] Human Mycoplasma pneumoniae liquid culture medium preparation:

[0055]

[0056] Preparation method is identical with embodiment 1.

Embodiment 3

[0058] Human Mycoplasma Pneumoniae Solid Medium—Preparation of Slide Type Culture Box:

[0059]

[0060]

[0061] Add to:

[0062]

[0063] Bacteriostats: 1 million units of ampicillin, 50 mg of natamycin.

[0064] After mixing evenly, perform aseptic operation, take 4-5ml into the culture box, cover it after cooling.

[0065] Other preparation methods are the same as in Example 1.

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Abstract

The invention relates to a human mycoplasma pneumoniae culture medium and a diagnosis kit thereof. The human mycoplasma pneumoniae culture medium comprises a TSB culture medium and an additive, wherein the additive comprises bovine serum, DMEM (dulbecco's modified eagle medium), avian yolk extract, sodium pyruvate and glutamine. The DMEM accounts for 5-15 vol% of the human mycoplasma pneumoniae culture medium. On the basis of the total volume of the human mycoplasma pneumoniae culture medium, the avian yolk extract content is 10-100 ml / L, the sodium pyruvate content is 0.2-1.0 g / L, and the glutamine content is 0.3-1.0 g / L. Compared with the prior art, the culture medium provided by the invention performs practical innovation design on the basis of abundant practices, conforms to the demands, ensures the specificity and reliability of the clinical Mp infection diagnosis, is convenient to use, and has the advantages of high speed, high safety, environment friendliness and resource saving.

Description

technical field [0001] The invention relates to a mycoplasma culture medium and a diagnostic kit thereof, in particular to a human mycoplasma pneumoniae culture medium and a diagnostic kit thereof. Background technique [0002] Mycoplasma, which has been discovered for nearly a hundred years, is a kind of prokaryotic microorganism without cell wall, which is between viruses and bacteria. At present, more than 150 species have been isolated. The main ones related to human diseases are Mycoplasma pneumoniae (Mp), Ureaplasma urealyticum (Uu for short) and Mycoplasma hominis (Mh for short). Mp has long been proved to be an important pathogenic bacteria of atypical pneumonia, which is difficult to diagnose, easily causes concurrent infection, and has a high mortality rate. After the SARS epidemic in 2003, when community respiratory infections became popular, it attracted more attention from the medical community. [0003] Mp can enter the blood and proliferate outside the resp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/35
CPCC12N1/20C12Q1/045
Inventor 文凤岐武济民孙亚洲肖家祁
Owner 众爱生河北生物科技有限公司
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