163 results about "Epidermal stem cell" patented technology

A method of processing an acellular tissue matrix to give a particulate acellular tissue matrix includes: cutting sheets of dry acellular tissue matrix into strips; cryofracturing the dry acellular tissue matrix strips at cryogenic temperatures; separating the resulting particles by size at cryogenic temperatures; and freeze drying the fraction of particles desired size to remove any moisture that may have been absorbed to give a dry particulate acellular tissue matrix. Rehydration of the dry particulate acellular tissue matrix may take place just prior to use. The particulate acellular tissue may be applied to a recipient site, by way of injection, spraying, layering, packing, in-casing or combinations thereof. The particulate acellular tissue may further include growth and stimulating agents selected from epidermal growth factor, fibroblast growth factor, nerve growth factor, keratinocyte growth factor, platelet derived growth factor, vasoactive intestinal peptide, stem cell factor, bone morphogetic proteins, chondrocyte growth factor and combinations thereof. Other pharmaceutically active compounds may be combined with the rehydrated particulate material including: analgesic drugs; hemostatic drugs; antibiotic drugs; local anesthetics and the like to enhance the acceptance of the implanted particulate material. The particulate material product may also be combined with stem cells selected from mesenchymal stem cells, epidermal stem cells, cartilage stem cells, hematopoietic stem cells and combinations thereof.

The invention provides a method using a CRISPR-Cas system to perform GING2 gene editing on an epidermal stem cell and particularly relates to a method for building an epidermal stem cell line with theGING2 gene being knocked out. Two specific gRNAs are built and acquired, and the GINS2 gene editing efficiency of CRISPR / Cas9 in the epidermal stem cell can be increased evidently. An epidermal stemcell GINS2 knockout plasmid is good in hereditary stability and high in targeting efficiency.

The invention relates to in-vitro inducing differentiation of umbilical cord mesenchymal stem cells into skin tissue engineering seed cells. The invention discloses a new research direction for human umbilical cord mesenchymal stem cells, i.e. in-vitro inducting differentiation into skin tissue engineering seed cells. The invention also discloses an experimental method thereof, which comprises the following steps: collecting the caesarean umbilical cords in aseptic environment; culturing original-generation umbilical cord mesenchymal stem cells (UCMSCs) by an enzyme digestion or tissue-piece adherent method; after purifying and subculturing to the third generation, inducing by using different in-vitro condition induction liquids to differentiate into epidermis stem cells and fibroblasts; and inducing and differentiating epidermis stem cells and fibroblasts into sudoriferous gland cells by using coculture mode with the normal sudoriferous cells. After the induced cells are digested, liquid nitrogen is stored for later use. The invention provides seed cells for skin tissue engineering, and has the advantages of strong multiplication capacity, easy inducing differentiation, low immunogenicity, abundant sources and easy obtainment.

The invention discloses an in-vitro recombined human skin epidermis model.A layer of hemidesmosome protein is arranged on the lower surface of a basal cell layer, the upper surface of the basal cell layer is covered with 6-8 spinous cell layers containing short protruding spinous cells, the upper surface of the spinous cell layers is covered with 3-4 granular cell layers containing transparent granular cells, the upper surface of the granular cell layers is covered with a keratinocyte layer, all the cell layers are obviously separated, and the overall barrier function of a lipid composition is approximate to that of human skin.A preparation method comprises the steps of preparation of matrigel particles, screening and amplification of epidermal stem cells, preparation of the basal cell layer, preparation of the spinous cell layers, preparation of the granular cell layers, and maturation and keratinization of the in-vitro recombined epidermis model.The model is applied to evaluation of cosmetics with the human skin barrier repair effect, screening of plant extract with the human skin barrier repair effect, evaluation and detection of irritation of medical apparatus materials on the human skin and detection of irritation of chemicals on the skin.

The invention relates to tissue-engineered skin containing blood vessels and hair follicle structures based on 3D printing and a preparation method thereof. The tissue-engineered skin is composed of an epidermal layer, an acellular dermal scaffold and a dermis layer, wherein in the epidermal layer, epidermal stem cells are used seed cells, the seed cells are printed on the upper surface of the acellular dermal scaffold through a 3D printer after being compounded with a hydrogel carrier to differentiate and form a normal epidermal structure, in the dermis layer, mesenchymal stem cells, vascularendothelial cells, dermal papilla cells and adipose-derived stem cells are used as seed cells, the seed cells and a hydrogel carrier are compounded, through the 3D printer, gelatin slow-release micropheres compounded with cytokines are printed on the lower surface of the acellular dermal scaffold, and meanwhile, the hydrogel compound of the seed cells is printed in the gelatin slow-release micropheres to form a dermis structure with a three-dimensional spatial structure.

The invention belongs to the technical field of biological cells, and relates to a method for separating and culturing human epidermal stem cells. The characteristics of the method include: preparation of a plate covered with extracellular matrix; separation of epidermal cells; preparation of trophoblast cells; screening and culturing of epidermal stem cells. At present, this method has been stably cultured and passaged in vitro for more than 120 PD. It has been confirmed by in vivo and in vitro experiments that the cells have no tumorigenicity, have strong proliferation ability, and can form mature full-thickness differentiated epidermis. The clinical application plays an immeasurable role, and at the same time breeds huge social and economic benefits.

The invention discloses a facial mask containing a garlic extract product. The facial mask comprises, by mass, 1 to 5% of propylene glycol, 3 to 7% of 1,3-butylene glycol, 0.1 to 0.2% of a thickening agent, 0.05 to 0.15% of formic ester, 50 to 70% of water, 0.02 to 0.06% of small molecular hyaluronic acid, 3 to 7% of propylene glycol, 0.1 to 0.3% of dipotassium glycyrrhizinate, 0.1 to 0.2% of triethanolamine, 1 to 5% of water, 0.5 to 1.5% of garlicin, 3 to 4% of a garlic extracted solution, 3 to 7% of a witch hazel extracted product, 1 to 5% of yeast extracted product, 0.5 to 1.5% of an epidermal stem cell activation factor, 1 to 3% of Sophora flavescens, 5 to 10% of water, and 2 to 3% of leupeptin. The facial mask is capable of inhibiting bacteria and diminishing inflammation, possesses inhibition and killing effect on a plurality of micrococcus, bacillus, fungus, and viruses, is capable of eliminating acne caused by skin inflammation and bacteria, supplying nutrients and trace elements, and improving immunity.

The invention discloses a method for inducing and acclimating epidermal stem cells into nerve cells, comprising the following steps of: mixing an eplife culture medium and an induction culture medium according to different proportions, and ensuring that the epidermal stem cells have enough time fit for the continuous reduction of the eplife culture medium and the gradual increase of the induction culture medium, which is beneficial to reducing the differentiation to the epidermis and increasing the transformational probability to targeted cells. The method is simple and very effective to directively induce and acclimate the epidermal stem cells into the nerve cells, overcomes the prejudice from technical personnel in the filed, subverts a judgment that the epidermal stem cells are unipotent stem cells for the first time, and proves that the epidermal stem cells have the multi-directional differentiation potential.

An anserum culture medium for cuticular layer stem cells of ganzhong milch goat can be prepared according to formula of F12+1-1.2% BSA+20-25% GSMCM+20-25ng / EGF+5ug / ml insulin+15-20ng / ml IGF=1+0.4-0.6 microng / ml hydrocortisone+penicillin and streptomycin 100u / ml. The culture medium preparing process includes adding insulin growth factor based on only adding cuticular layer factor in traditional method, applying method of adding fibroblast condition culture medium in stead of method in using fibroblast for cocultivating.