164 results about "Epidermal stem cell" patented technology

A method of processing an acellular tissue matrix to give a particulate acellular tissue matrix includes: cutting sheets of dry acellular tissue matrix into strips; cryofracturing the dry acellular tissue matrix strips at cryogenic temperatures; separating the resulting particles by size at cryogenic temperatures; and freeze drying the fraction of particles desired size to remove any moisture that may have been absorbed to give a dry particulate acellular tissue matrix. Rehydration of the dry particulate acellular tissue matrix may take place just prior to use. The particulate acellular tissue may be applied to a recipient site, by way of injection, spraying, layering, packing, in-casing or combinations thereof. The particulate acellular tissue may further include growth and stimulating agents selected from epidermal growth factor, fibroblast growth factor, nerve growth factor, keratinocyte growth factor, platelet derived growth factor, vasoactive intestinal peptide, stem cell factor, bone morphogetic proteins, chondrocyte growth factor and combinations thereof. Other pharmaceutically active compounds may be combined with the rehydrated particulate material including: analgesic drugs; hemostatic drugs; antibiotic drugs; local anesthetics and the like to enhance the acceptance of the implanted particulate material. The particulate material product may also be combined with stem cells selected from mesenchymal stem cells, epidermal stem cells, cartilage stem cells, hematopoietic stem cells and combinations thereof.

The invention belongs to the technical field of biological cells, and relates to a method for separating and culturing human epidermal stem cells. The characteristics of the method include: preparation of a plate covered with extracellular matrix; separation of epidermal cells; preparation of trophoblast cells; screening and culturing of epidermal stem cells. At present, this method has been stably cultured and passaged in vitro for more than 120 PD. It has been confirmed by in vivo and in vitro experiments that the cells have no tumorigenicity, have strong proliferation ability, and can form mature full-thickness differentiated epidermis. The clinical application plays an immeasurable role, and at the same time breeds huge social and economic benefits.