Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo

A technology derived from hair follicle stem cells and embryos, applied in the field of stem cells and genetic engineering, can solve the problems of harsh culture conditions and slow proliferation of epidermal stem cells

Inactive Publication Date: 2006-09-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology allows us to quickly grow large numbers of hairy cell types from our own body called Eden's Hair Folly MSC(Evans). These small units are similar enough that they have different characteristics such as growth speed or ability to repair damaged tissue. They could potentially help treat medical conditions like burn injuries by providing them back into their original form when needed.

Problems solved by technology

This patents discusses how biological processes involve stimulation between certain types of nerves called neurons, specifically epithelial neural crest cells. While there may exist several ways to improve these processes including improving their effectiveness, reducing damage caused due to external factors like sunlight exposure, heat injury, radiation therapy, chemotherapies, and inflammatory responses. Current techniques include surgery, grafting, plasma donor injection, eletrospun fibre bundles, platelet activation, and histochirculated cell migration.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo
  • Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo
  • Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of total RNA from human epidermal cells:

[0026] Epidermal cells were obtained from adult human skin through primary culture (Yang Ling, Liu Houqi, etc. Localization, isolation and culture of epidermal stem cells from adult human skin. Journal of Second Military Medical University, 2004, 25(8): 815-817.). The total RNA was obtained by extracting the total RNA by the conventional guanidine isothiocyanate method with the total RNA extraction kit of Shanghai Huashun Bioengineering Co., Ltd.

[0027] (2) Synthesis of primers and construction of pCDNA3.0-EGF eukaryotic expression vector:

[0028] The hEGF gene was transferred to the eukaryotic vector pCDNA3.0. Obtain the cDNA fragment containing the EGF gene according to the literature and recombine it into the eukaryotic expression vector pCDNA3.0 (refer to Chen Tingsu et al., Synthesis and identification of human epidermal growth factor (hEGF) gene and construction of plant expression plasmid, Journal of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention provides a method for accelerating the proliferation of human mesenchymal stem cells, which comprises the steps of: transfecting recombinant expression carrier containing EGF to human mesenchymal stem cells, and stimulating with a certain concentration of calcium ions during the transfection process to divide into typical stem cells. Under nude mouse subcutaneous microenvironment induction, the stem cells are further divided into skin tissue stem cells, thus directly taking part in the proliferation of nude mouse hair. This invention can obtain rapidly proliferated human mesenchymal stem cells, thus having a potential application in clinical skin wound treatment.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com