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Application of glacial water in preparation of externally applied agent for skin and glacial water-containing externally applied agent for skin

An external skin preparation, glacier technology, applied to the application of glacier water in the preparation of external skin preparations and the field of external skin preparations containing it, can solve the problems of patent applications and literature for external skin preparations

Inactive Publication Date: 2016-04-20
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Based on the above-mentioned literature and patent research, there are no patent applications and literature on the preparation of glacier water for external skin preparations with anti-aging and whitening effects

Method used

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  • Application of glacial water in preparation of externally applied agent for skin and glacial water-containing externally applied agent for skin
  • Application of glacial water in preparation of externally applied agent for skin and glacial water-containing externally applied agent for skin
  • Application of glacial water in preparation of externally applied agent for skin and glacial water-containing externally applied agent for skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The culture of embodiment 1 people's primary fibroblasts

[0067] Cell isolation: Human skin tissue was obtained from human foreskin excised by the Department of Urology, Ruijin Hospital, Shanghai. The excised tissue was rinsed repeatedly in D-Hanks buffer containing penicillin (100kU / L) and streptomycin (100mg / L), and then the subcutaneous fat was removed. Cut the skin to 0.5 cm with ophthalmic scissors in a Petri dish 2 Small pieces of left and right and spread the epidermis down in a petri dish, add a little 1640 medium and 10% FBS at 37°C, 5% CO 2 After culturing for several weeks under the same conditions, the skin tissue was taken out after the fibroblasts adhered to the wall. Cells can be used for subsequent experiments after subculture for several weeks, and the medium is changed every 3-4 days.

[0068] Cell subculture: when the confluence rate of primary cultured fibroblasts reaches 90%, add 0.25% trypsin to the culture flask, when the cytoplasm shrinks, th...

Embodiment 2

[0069] The proliferative activity of embodiment 2MTT assay cell

[0070] The fibroblasts in the logarithmic growth phase in Example 1 were digested and diluted, and the cell concentration was adjusted to be 2×10 4 / ml. Inoculate in a 96-well culture plate containing 100 μL of culture solution, 100 μL per well, set up 3 replicate wells each time, culture for 4, 6, 8, 10, 12, and 24 hours respectively, add 50 μL of MTT solution to each well and continue to incubate for 4 hours , Aspirate the culture medium, add 150 μL dimethyl sulfoxide (Dimethylsulfoxide or DMSO) to each well, shake for 10 minutes, select a wavelength of 490 nm, and measure the optical density (OD) value of each well. The experiment was carried out 3 times, and the results were averaged.

[0071] Human primary skin fibroblasts (HSF) were treated with 1640 medium prepared with glacier water, and 1640 medium prepared with ultrapure water was used as a control, and detected by MTT method at 4, 6, 8, 10, 12, and ...

Embodiment 3

[0074] Embodiment 3 experimental animals and administration

[0075] Aged C57bl6 mice were provided by the Experimental Animal Center of Shanghai Jiaotong University School of Pharmacy. The animal production and use license numbers are SCXK (Shanghai) 2003-0003 and SYXK (Shanghai) 2007-0025, respectively.

[0076] 6 mice were randomly selected, and their backs were depilated. Ultrapure water and glacier water were applied to the back skin of each mouse 3 times a day, respectively, for one month.

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Abstract

The invention discloses application of glacial water in preparation of an externally applied agent for the skin and a glacial water-containing externally applied agent for the skin. It is found for the first time that glacial water can obviously promote propagation of fibroblasts, improve synthesis of fibroblast collagen I and elastin laminin of the skin and substantially inhibit propagation activity of human epidermal melanocytes (HEM-m) and formation of melanin. Glacial water is used as one or more selected from the group consisting of an anti-aging active component, a whitening active component and a moisture-retaining component of the externally applied agent for the skin; so the glacial water-containing externally applied agent for the skin is natural and safe and is capable of improving skin elasticity, resisting crease, repairing impaired skin, reducing hyperpigmentation and whitening the skin.

Description

technical field [0001] The present invention relates to the application of glacier water in the preparation of skin external preparations and the skin external preparations containing it. Background technique [0002] Glacier water is a natural ice body that exists on the surface for a long time and can move by itself. Accumulated from atmospheric solid precipitation over many years, it is an important fresh water resource on the surface, which is different from frozen water in rivers and lakes in winter. After new snow falls to the ground, the unmelted snow after a melting season is called granular snow. The snow is gradually dense, melted and then frozen, so that the crystals merge, the size and shape of the crystal grains change, and directional growth occurs. When its density reaches a critical value, air permeability and water permeability are promoted between the grains, and it becomes glacier ice. Scientists in many countries have conducted a series of studies on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/96A61Q19/00A61Q19/08A61Q19/02
CPCY02A50/30
Inventor 金家宏陈明华黄晓青曹平
Owner JALA GROUP CORPORATION
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