215 results about "Melanocyte production" patented technology

The invention discloses a compound traditional Chinese medicine extract and application thereof to a whitening, moisturizing and anti-aging skin care product. The compound traditional Chinese medicine extract is prepared from the following raw material medicines in parts by mass: 10-50 parts of selaginella, 10-50 parts of peach blossom, 10-50 parts of bletilla and 10-50 parts of pseudo-ginseng. The compound traditional Chinese medicine extract which is prepared from the raw materials has an effect of suppressing tyrosinase and proliferation of melanocyte, has a superior whitening effect, has an effect of proliferating and promoting fibroblast and has an excellent effect of promoting generation of ceramide. The whitening, moisturizing and anti-aging skin care product which is obtained by applying the compound traditional Chinese medicine extract to preparing the whitening, moisturizing and anti-aging skin care product has a durable natural whitening effect and can be used for improvingmetabolism and moisturizing effects of skin.

The invention relates to a method for cosmetically treating dark spots on the skin, wherein said method is intended for eliminating at least one of said dark spots located in a region of the hand, the face, the limbs or the chest area of a person suffering from such skin hyperpigmentation, characterised in that said method includes a step of applying a spray of cryogenic fluid to said region, which brings the skin temperature to a temperature of between −4° C. and −15° C. for a consecutive application period of from 2 to 10 seconds, in order to selectively act on the melanocytes, and to a device for carrying out said method.

Methods and formulations are provided to reduce pigmentation in skin, using an array of compounds selected from benzimidazoles, phenylthioureas, phenyltiols, phenylamines, bi- and multicyclic phenols, thiopheneamines, and benzothiamides. The compounds preferably inhibit pigment systhesis in melanocytes through the tyrosinase pathway. The methods can be used for lightening skin, and for treating uneven skin complexions which result from hyperpigmentation-related medical conditions such as melasma, age spots, freckles, ochronosis, and lentigo. The compounds can be used medically or cosmetically.

The invention relates to an experimental method for analyzing the toxicity and the effect of a skin whitening agent through a primary culture human being skin melanocyte. The method comprises the following steps: 1, culturing and identifying the primary human being skin melanocyte; 2, carrying out the analysis of the toxicity of skin whitening substances through the primary human being skin melanocyte; and 3, carrying out the analysis of the effect of the skin whitening substances through the primary human being skin melanocyte, namely carrying out the analysis on two aspects of the melanin content and the tyrosinase activity. The method has the following advantages that: the human being melanocyte cultured in vitro has a high division and multiplication capacity, a high standardization degree, little difference between batches, and the same function of producing melanin granules with the melanocyte in vivo; the result obtained through the healthy human being melanocyte is more reliable than the result obtained through animal or human-derived melanoma cells; and the toxic action and the whitening effect of the substances to be tested are evaluated from the two aspects of characterization and quantification. The method can replace the living body animal and human being skin, and can be directly applied to toxicity and effect experiments of the whitening substances in products such as chemicals, cosmetics, medicaments, and the like.

The present invention relates to a functional pigmented skin equivalent. The invention relates to an in vivo skin equivalent which is characterized by comprising at least one epidermis equivalent and at least one dermis equivalent. The in vivo skin equivalent further comprises melanocytes constitutively producing melanin and fibroblasts. The invention also relates to a method for preparing the skin equivalent.

Embodiments of methods and apparatus use electron paramagnetic resonance spectroscopy to provide a signal from melanin to image a melanoma. Embodiments of methods and apparatus use electron paramagnetic resonance spectroscopy to provide a signal from melanin to detect metastatic melanoma in a sentinel lymph node. Embodiments of methods and apparatus use electron paramagnetic resonance spectroscopy to provide a signal from melanin to measure light penetration in melanocytes in skin.

The invention provides a marine bio-moisturizing and anti-aging cosmetic composition. The composition uses jellyfish extract and fucus extract as active ingredients and comprises 5 to 15% by weight ofjellyfish extract and 5 to 15% by weight of fucus extract. Through combination of jellyfish extract and fucus extract as active ingredients and other extract and a cosmetic matrix, the production offree radicals is reduced through supplementing antioxidant substances. Collagen or enzymes are supplied so that the DPPH free radicals are effectively eliminated, the tyrosinase activity is greatly inhibited, the biosynthesis of melanin in melanocytes is reduced and anti-aging effects are obtained. The marine bio-moisturizing and anti-aging cosmetic composition is natural and environmentally friendly and can well protect the skin.

The invention discloses a traditional Chinese medicine composition with whitening and anti-aging effects and a preparation method and application of an extract thereof. The traditional Chinese medicine composition disclosed by the invention is prepared from the following ingredients in percentage by weight: 1-30% of radix asparagi, 1-30% of evening primrose, 3-50% of chaenomeles lagenaria, 3-50% of pomegranate peel, 1-30% of giant knotweed rhizome, 3-40% of herba selaginellae, 2-30% of cynomorium songaricum and 2-40% of oldenlandia diffusa. The extract of the traditional Chinese medicine composition disclosed by the invention plays roles in inhibiting melanocytes, inhibiting tyrosinase and resisting free radicals and ultraviolet rays, can be used for effectively inhibiting the generation of skin melanin, and has excellent whitening and anti-aging effects. The composition does not contain chemically-synthesized substances, is free from toxic or side effects, is low in skin irritation and stable in nature, and is safe and reliable.

The invention discloses a skin whitening composition. The skin whitening composition contains tranexamic acid, ascorbic acid ethyl ether, vitamin B3, N-(omega-undecylene acyl) phenylalanine, chamomile extract, dunaliella tertiolecta extract (IBR-CLC 0402), methylene bis-benzotriazolyl tetramethyl butyl phenol, coenzyme Q10, purslane extract, alpha-red myrrh alcohol, beta glucan, clove oil, azone, 1,3-butanediol solvent as well as 1,2-octylene glycol and ethylhexylglycerin antiseptics. The active ingredients in the skin whitening composition can inhibit activity of tyrosinase, reduce formed melanin, inhibit migration of melanin globules, block information transmission between keratinocytes and melanocytes, promote falling off of cuticle, absorb ultraviolet rays, eliminate free radicals and resist inflammation. According to the skin whitening composition, each component is significant in synergic whitening effect, and the skin whitening composition is safe and stable and can be applied to skin whitening cosmetics.

The invention relates to band pigment labor epidermis substitute manufacturing method and its application. Its feature is that the used cell contains epidermis cell and melanocyte. Its manufacturing method are dissolving chitosan by 1% acetic acid; dissolving gelatine by distilled water; mixing them; heating them on plane dish, drying to form film material; taking it out; cleaning and dipping by a lot of distilled water; heating; setting in culture solution; setting the upper cell-film material compound in the culture solution. Compared with the current technique, the invention can repair epithelial tissue of the wound par, at the same time repair the melanocyte.

The invention discloses a melanocyte culture method. The method comprises the following step: preculturing for 2-4 hours before melanocyte enrichment culture, wherein the preculture medium is prepared by mixing a DMEM (dulbecco's modified eagle medium) culture medium and a Ham's F-12 culture medium in a volume ratio of (2-4):1, and is composed of 1.6-2.0*10<-4>M adenine, 80-100 unit/mL penicillin, 80-100 mu g/mL streptomycin, 0.1-0.5 mu g/mL hydrocortisone, 2-6 mu g/mL insulin, 5-12 ng/mL epidermal growth factor, 0.8-1.5*10<-10>M choleratoxin and 4-7% of fetal calf serum. The preculture in the method promotes wall adherence of melanocytes, and enhances the proportion and cell activity of the melanocytes. After the subculture, the melanocyte content is enhanced from 1% to 90% at the maximum, and the melanocytes have stable shape, functions and hereditary characters.

The invention relates to an ex vivo method for obtaining a population of human melanocytes derived from human pluripotent stem cells comprising the step consisting of co-culturing human pluripotent stem cells with cells that support ectodermal differentiation in the presence of an agent that stimulates epidermal induction and an agent that stimulates terminal differentiation of keratinocytes. The invention also relates to human melanocytes obtainable by said method and to uses thereof in cell therapy and in screening assays.

The invention relates to application of prinsepiautilis Royle tender bud extractive to a whitening product and the whitening product containing prinsepiautilis Royle tender bud extractive and/or prinsepiol. Polyphenols in the prinsepiautilis Royle tender bud extractive are a kind of new whitening active compositions, have obvious effects of inhibiting tyrosinase activity and reducing melanin generated by B16 melanocyte, and also the whitening active compositions come from plant, and therefore the prinsepiautilis Royle tender bud extractive is good in safety, and has extremely high feasibility of developing external-use medicines or beautifying cosmetics for whitening, spot removing and the like.

The present invention relates to a pharmaceutical composition for cell therapy of pigmentation disorders, which comprises as an active ingredient (a) melanocytes and keratinocytes, or (b) melanocytes and fibroblasts. The cell therapy composition of this invention allows for the first time to treat pigmentation disorders through cell therapy regimen. The present composition comprises as active ingredients a mixed cell preparation of melanocytes and keratinocytes or a mixed cell preparation of melanocytes and fibroblasts such that it induces very effectively pigmentation to treat diseases associated with hypopig mentation such as vitiligo.

The invention provides an immunohistochemical hematoxylin-toluidine blue double-redyeing method for melanocyte. The method comprises the following steps: (1), carrying out conventional xylene dewaxing on a slice and carrying out gradient alcohol dehydration; (2), blocking and inactivating endogenous peroxide enzyme; (3), repairing an antigen; (4), carrying out serum sealing; (5), dropwise adding primary antibodies; (6) dropwise adding secondary antibodies; (7), carrying out DAB developing; (8), carrying out hematoxylin redyeing and hydrochloric acid alcohol differentiation; (9), redyeing by a toluidine blue working solution and carrying out glacial acetic acid differentiation; (10), dehydrating to be transparent and sealing the slice. According to the invention, positive granules are still brownish yellow after redyeing, and original melanin granules in the tissue become deep green, so that distinction is obvious, the melanin granules are easily distinguished from brown granules, the developing of hematoxylin is not influenced, cell nucleuses of background cells are clearly visible, the color difference of immunohistochemical positive brownish yellow granules, deep green melanin granules and blue cell nucleus is obvious and intuitive, and distinguishing is convenient. Toluidine blue dyeing liquid is prepared simply and conveniently.

The invention discloses a plant whitening and freckle-removal mask and a preparation method thereof. The plant whitening and freckle-removal mask is prepared from the following components in parts byweight: 10 to 12 parts of rose extract, 5 to 7 parts of silkworm larva extract, 15 to 20 parts of mulberry leaf extract, and 15 to 20 parts of lavender extract. The plant whitening and freckle-removalmask has the advantages that the metabolism of skin surface cells is activated, the content of SOD (superoxide dismutase) is increased, the skin is fine, the skin pores are shrunk, the moisturizing is kept, the water is sustained, the synthesis of collagen protein is promoted, the elasticity of the skin is enhanced, the wrinkles are removed, and the effects of beautifying face and skin, as well as moisturizing and preventing wrinkles are realized; the generation of melanocyte can be inhibited, the activity of tyrosinase is decreased, the chloasma, freckle, cyasma, senile plaque and acne scarare removed, and the effects of fading melanocyte, removing freckle and whitening are realized.

The invention provides methods for improving the appearance and/or health of skin using topical compositions that include the tripeptide MIF, which has the chemical formula prolyl-leucyl-glycinamide, and/or related peptides. Additional aspects of the invention provide cosmetic and pharmaceutical compositions for topical application that contain MIF or related peptides.