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Stem cell exosome capable of repairing skin as well as preparation method and application thereof

A technology for repairing skin and exosomes, applied in the field of biological cells, can solve problems such as skin repair, achieve the effects of regulating inflammatory response, promoting regeneration and migration of skin-related cells, and reducing the production of melanin

Inactive Publication Date: 2018-11-20
XIAMEN ASSAY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing sunscreen substances only have a certain effect on inhibiting melanin production, but it is difficult to play a good repairing effect on sunburned skin

Method used

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  • Stem cell exosome capable of repairing skin as well as preparation method and application thereof
  • Stem cell exosome capable of repairing skin as well as preparation method and application thereof
  • Stem cell exosome capable of repairing skin as well as preparation method and application thereof

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preparation example Construction

[0039] 3. Preparation of stem cell exosomes that can repair skin

[0040] Will 2×10 9 The exosomes of particles were dissolved in 400 μl of PBS buffer containing 50 mM trehalose (Terahorse) (4°C) to form a mixture M1;

[0041] 1 mg of Decapeptide-12 (Lumixyl TM , Envy Medical, Inc. Westlake Village, USA) and the mixed solution M1 were added to a 0.4cm (volume 750μl) electroporation dish, placed in an electroporation apparatus, and electroporation transduction was performed under the conditions of a voltage of 400V and a capacitance of 250μF;

[0042]After transduction, wash twice with PBS buffer (4°C, centrifuge at 100,000 g for 2 h), resuspend the pellet with 200 μl of PBS buffer solution containing 30 mM trehalose, and obtain stem cell exosomes that can repair the skin;

[0043] The obtained skin-repairing stem cell exosomes were frozen at -80°C for future use.

[0044] Since Decapeptide-12 contains 1 tryptophan residue and 3 tyrosine (Tyr) residues, the stem cells prepar...

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Abstract

The invention provides a stem cell exosome capable of repairing skin as well as a preparation method and application thereof. The preparation method comprises the following step: transducing decapeptide-12 into mesenchymal stem cell exosome, thereby obtaining the stem cell exosome capable of repairing skin. According to the stem cell exosome capable of repairing skin prepared by the method disclosed by the invention, the invention first discovers that, by binding the mesenchymal stem cell exosome and decapeptide-12 and by utilizing loading capacity and low immunogenicity of exosome cells, thedecapeptide-12 can reach melanocyte so as to further inhibit tyrosinase activity, the inflammatory response can be regulated, and skin related cell regeneration is promoted. The stem cell exosome capable of repairing skin prepared by the invention can achieve effects of eliminating erythema and edema when applied to sun-burned skin, can reduce melanogenesis and further reduce pigmentation and formation of black spots after sunburn, can be applied to the fields of cosmetics, medicines for treating skin diseases and the like, and has obvious effects and important application value.

Description

technical field [0001] The invention relates to the technical field of biological cells, in particular to a skin-repairing stem cell exosome and its preparation method and application. Background technique [0002] Sunlight is composed of light waves of different wavelengths, among which, medium-wave ultraviolet (UVB) can reach the basal layer of the epidermis. Strong exposure to sunlight will cause epidermal keratinocyte necrosis and release inflammatory mediators such as histamine, serotonin, and kinin , which in turn leads to dermal vasodilation and tissue edema, followed by melanocytes accelerating the synthesis of pigments, deepening skin pigmentation. Skin sunburn after sun exposure mainly manifests as erythema, edema or blisters on the local skin, and pigmentation spots remain after healing, accompanied by burning and pain. [0003] Studies have shown that UVB can not only directly damage cells, but also the damaged microRNA released by damaged cells will be released...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61K9/19A61P17/00A61P29/00A61K8/99A61K8/64A61Q19/00A61K35/28
CPCA61K8/64A61K8/99A61K9/19A61K35/28A61K38/08A61K2800/782A61Q19/004A61P17/00A61P29/00A61K2300/00
Inventor 孙坪田云鹏李柱
Owner XIAMEN ASSAY BIOTECH CO LTD
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