The invention relates to a preparation method of a bactericidal hand wash detergent for clothes of pregnant women. Skin of expectant mothers in pregnancy can get sensitive, improperly selected washing products enable the pregnant women to suffer from an allergy and can become potential carcinogenic factors, health of the expectant mothers is injured, and growth of a fetus is also indirectly affected. The bactericidal hand wash detergent is prepared from a wild pagoda tree root extract, tea saponin, a radix puerariae extract, a pine bark extract, blumea oil, a ginseng extract, AES (sodium alcohol ether sulphate), probiotic flora, paeonol, chitosan, citric acid, amino acid, coconut oil fatty acid, a magnolin extracting solution, ursolic acid, chitin, apple essential oil, acetic acid, spice and water through mixing. In order to decompose common stains (sweat stains, blood stains and milk stains) in the pregnant and puerperal period, the bactericidal hand wash detergent has cleaning, softening and bactericidal functions, is higher in detergency and enables clothes to be cleaner and whiter.