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Schizochytrium WZU4771, and application in preparing powder of DHA and grease

A technology of schizochytrium and WZU4771, applied in the field of microorganisms, can solve the problems of industrialization limitations and low DHA productivity, and achieve the effect of simple long-chain polyunsaturated fatty acid spectrum

Active Publication Date: 2007-02-21
XIAMEN HUISON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the DHA productivity achieved by Thraustochytrium is not very high at present, and its industrialization is greatly restricted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take the rotten leaves from the tidal flat reeds of Qidu Island, Oujiangkou, Wenzhou City, Zhejiang Province, and cut them into small discs with a diameter of about 1.5 cm or fragments with a length of 1.5 cm. Wash with sterile sea water, inoculate it on the said preliminary screening medium plate, add about 5 ml of sterile sea water and a small amount of sterile pine pollen on the plate, and cultivate for 4-5 days at 25°C. Pick pine pollen, observe the culture that adheres to the pine pollen, transfer the pine pollen of the adherent culture to a new plate for cultivation, until a pure culture is obtained.

[0034] The pure culture was inoculated into a 250 ml Erlenmeyer flask containing 50 ml of the re-screening medium, and cultured on a shaker at 150 rpm for 3 days at a culture temperature of 25°C. The culture was centrifuged at 3500 rpm for 10 minutes, washed with water and then centrifuged, dried at 105°C for 3 hours, and the dry weight of the cells was weighed.

[0035...

Embodiment 2

[0040] Several different carbon sources were selected, with a concentration of 10%, 0.8% of peptone as a nitrogen source, a seawater concentration of 50%, and a pH of 6. The inoculum was 10%, the culture temperature was 25°C, and the shaker rotation speed was 150 rpm, and the DHA fermentation of Schizochytrium WZU4771 was performed. Cultivate for 5 days, the result is as

[0041] As shown in Table 1, glucose is the best carbon source.

[0042] Carbon source

Embodiment 3

[0044] Several different nitrogen sources were selected, with a concentration of 0.8%, 10% glucose as a carbon source, seawater concentration at 50%, and a pH of 6. The inoculum was 10%, the culture temperature was 25°C, and the shaker rotation speed was 150 rpm, and the DHA fermentation of Schizochytrium WZU4771 was performed. After 5 days of cultivation, the results are shown in Table 2. Peptone is the best nitrogen source.

[0045] Nitrogen source

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PUM

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Abstract

This invention discloses Schizochytrium WZU477 and its application in preparing DHA powder and DHA grease. The Schizochytrium WZU477 is obtained by gathering rotten leaves and using pine pollen as the bait, and is preserved at China General Microbiological Culture Collection Center (CGMCC No. 1730). The Schizochytrium WZU477 can produce fermented liquid that has 42.5 g / L cell dry weight and 14.1 g / L DHA acid after cultured for 5 d under optimal conditions. The productivity of DHA is 2.76 g / L .d, twice that of previously reported DHA production.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a new Schizochytrium and its application. Background technique [0002] Docosahexaenoic acid (cis-4,7,10,13,16,19-docosahexaenoic acid, DHA for short), commonly known as brain gold, is an important structural substance of the brain and retina. It can also regulate the function of the central nervous system and prevent And treat cardiovascular diseases, eliminate inflammation, and inhibit cancer. For a long time, the commercial sources of DHA have been fish oil and microalgae. The yield of fish oil and DHA content are both unstable, and the extract contains fishy smell. The cultivation of microalgae requires light and carbon dioxide, and the production process is complicated and costly. As the population increases, the demand for DHA increases accordingly, and it is necessary to find a new source of DHA with large quantity and high quality. Currently considered as pot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12P7/64
Inventor 赵肖为周茂洪
Owner XIAMEN HUISON BIOTECH
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