36 results about "Protist" patented technology

Methods and means for efficiently downregulating the expression of a target gene of interest in cell from an organism that is an animal, fungus and protist. The invention provides chimeric nucleic acid molecules for downregulating target genes. The invention also provides modified cells and organisms comprising the chimeric nucleic acid molecules and compositions comprising the chimeric molecules.

The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.

The invention discloses a method for determining microbial species in a sample. The method comprises the following steps: sequencing at least one part of nucleic acids in the to-be-detected sample, so as to obtain sequencing data; performing alignment of the sequencing data with a first reference sequence, wherein the first reference sequence is a known sequence which is derived from the to-be-detected sample and belongs to an individual organism category, and acquiring a read which meets at least one of (1)-(3), wherein (1) the read is not subjected to alignment with the first reference sequence, (2) the similarity, with the first reference sequence, of the read is less than a preset proportion, and (3) the read is subjected to alignment with the first reference sequence and alignment quality is lower than a threshold; and performing alignment of the obtained read with a microbial reference sequence, and determining the microbial species in the to-be-detected sample in accordance with an acquired alignment result, wherein the microbial reference sequence includes at least two of bacterium, fungus, virus and protist reference sequences. The invention also discloses a device for determining the microbial species in the sample and a method for detecting pathogenic microorganisms.

The invention provides a filter composite layer and a filter blocking net, and application method thereof. The composite filter layer comprises a filter layer and a reinforcement layer. The filter layer is a pure polyester or polypropylene short fiber spunlaced non-woven fabric. The reinforcement layer is a terylene industrial filament interlacing netted woven fabric or warp knitted fabric. The reinforcement layer is arranged in the filter layer. The reinforcement layer and the filter layer are combined together as one spunlaced layer. The filter composite layer and filter blocking net, and application method thereof provided by the invention can effectively clean duckweed, algae and other protist on surface of drinking water reservoir and urban river and lake, so as to avoid water pollution caused by aquatic protist and to ensure water quality safety.

The invention discloses a prawn and grass symbiotic culture method. The prawn and grass symbiotic culture method comprises the following steps that S1, a 6 x 3 x 3 natural pond is built, water is dissolved with quicklime, the quicklime is uniformly sprayed to disinfect, the whole pond is disinfected with niclosamide 340 after 10 days, disinfecting is conducted for 5 days, a water inlet valve is arranged, aquatic plants with stem length of more than 10cm is selected, the aquatic plants is planted on a floating mat, continuously culturing is conducted for 3-4 days, and prawn seedlings are placed; and S2, dissolved oxygen content, ammonia content and nitrogen content of water are monitored, and the number of prawns and the aquatic plants and shelling condition, exercise amount and food intake of the prawns are observed by using an underwater camera, a reinforcement learning algorithm is adopted, and according to the dissolved oxygen content, the ammonia content and the nitrogen content of the water and the number of the prawns and the aquatic plants, the putting amount and the illumination degree of drugs or fertilizer and water grass culture products for inhibiting protists and oxygenates are added. According to the prawn and grass symbiotic culture method, a deep learning model and a reinforcement learning model are used for automatic dynamic adjustment, macroscopic regulation and control and real-time adjustment are achieved, manual control is not needed, and aquaculture achievement of the prawns and the aquatic plants can be greatly improved.

The present invention provides a method for introducing a functional peptide encoded by a plant or protist nucleic acid sequence into a mitochondrion of a mammalian cell, and a pharmaceutical composition comprising the nucleic acid sequence. The present invention also provides a method for correcting a phenotypic deficiency in a mammal resulting from a mutation in a mitochondrial peptide. Additionally, the present invention is directed to a method for treating a mitochondrial disorder in a subject in need of treatment therefor. The present invention further provides expression vectors for use in introducing a functional peptide encoded by a plant or protist (including algal) nucleic acid sequence into a mitochondrion of a mammal, as well as mammalian cells transformed by the expression vectors. Also provided are clonal cell strains comprising the transformed mammalian cells. Finally, the present invention is directed to a method for introducing a functional peptide into a mitochondrion.

Methods of detecting and treating rapidly growing exogenous cells, such as Protista, or parasites, that preferentially accumulate a photoactivatable porphyrin in which 5-aminolevulinic acid or precursor thereof is administered to the patient, or contacted to the exogenous cells, in an amount sufficient to induce synthesis fluorescence and/or photosensitizing concentrations of a protoporphyrin IX in the exogenous cells, followed by exposure of the exogenous cells to light of photoactivating wavelengths.

The object of the present invention is to separate and provide a β-glucosidase gene having the effect of efficiently promoting saccharification in hydrolysis of cellulose-containing biomass from a hardly culturable symbiotic protist community of Coptotermes formosanus, and the present invention specifically relates to β-glucosidase derived from a protist of the genus Pseudotrichonympha consisting of the amino acid sequence represented by SEQ ID NO: 1.